1. Suppose you're running a protein gel. You perform a protein assay on your sample, with 20 µl protein sample and 180 μl working solution. You measure a protein concentration of 40 µg/ml in the assay tube. What is the protein concentration in your sample tube (in μg/ml)? 2. Continuing the same sample, suppose you mix 65 μl of protein sample, 25 μl of sample buffer, and 10 μl of sample reducing agent in a new tube (gel-ready). What is the protein concentration in your gel-ready tube (in μg/ml)? 3. Continuing the same gel-ready sample, suppose you load 20 µl of protein on the gel. How much protein is that (in μg)? 4. Continuing with the same gel-ready sample, suppose you want to load 6.0 µg of protein on the gel. How much is that in μl?

1. Suppose you're running a protein gel. You perform a protein assay on your sample, with 20 µl protein sample and 180 μl working solution. You measure a protein concentration of 40 µg/ml in the assay tube. What is the protein concentration in your sample tube (in μg/ml)? 2. Continuing the same sample, suppose you mix 65 μl of protein sample, 25 μl of sample buffer, and 10 μl of sample reducing agent in a new tube (gel-ready). What is the protein concentration in your gel-ready tube (in μg/ml)? 3. Continuing the same gel-ready sample, suppose you load 20 µl of protein on the gel. How much protein is that (in μg)? 4. Continuing with the same gel-ready sample, suppose you want to load 6.0 µg of protein on the gel. How much is that in μl?

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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