1. Suppose you're running a protein gel. You perform a protein assay on your sample, with 20 µl protein sample and 180 μl working solution. You measure a protein concentration of 40 µg/ml in the assay tube. What is the protein concentration in your sample tube (in μg/ml)? 2. Continuing the same sample, suppose you mix 65 μl of protein sample, 25 μl of sample buffer, and 10 μl of sample reducing agent in a new tube (gel-ready). What is the protein concentration in your gel-ready tube (in μg/ml)? 3. Continuing the same gel-ready sample, suppose you load 20 µl of protein on the gel. How much protein is that (in μg)? 4. Continuing with the same gel-ready sample, suppose you want to load 6.0 µg of protein on the gel. How much is that in μl?

Question 12 (5.333 points) Listen A 1-year-old child weighing 26 lbs is prescribed ibuprofen 60 mg orally every 6 hours. The nurse has ibuprofen suspension 100 mg/5 mL on hand. The safe dose range is 5-10 mg/kg every 6-8 hours for a maximum of 40 mg/kg/day Blank 1: Calculate the single safe dose range in mg/dose: (round to nearest tenth) Blank 2: How many mL would this dose be to administer? (round to nearest tenth) Blank 3: Should the nurse administer this dose (yes/no, if no, why?) Blank # 1 Blank # 2 Blank #3 Question 13 (4.444 points) Listen A/ A 38 lb child is prescribed phenobarbital 10 mg orally every 12 hours. The nurse has phenobarbital 15 mg/5 mL in the med cart. The SDR is 1 to 6 mg /kg daily in two divided doses not to exceed 100 mg/day.

Question 14 Friedreich's ataxia is a rare inherited disease that causes nervous system damage and movement problems. It usually begins in childhood and leads to impaired muscle coordination (ataxia) that worsens over time. Friedreich's ataxia is caused by an insertion of several copies of a three nucleotide sequence in a gene called EXN. Which genetic testing method would be most appropriate for testing for Freidreich's ataxia? PCR with gel electrophoresis O PCR with conventional sequencing O PCR with SNP-chip O PCR with whole-genome sequencing

Question 2 You are checking a calculation that a foundation pharmacy student has performed. The question asks that 120 suppositories weighing 1 gram are to be made, containing 300mg Zinc Oxide. The displacement value of ZnO is 4.7 in the base that is to be used. How many grams of base would be required?  units - g Answer to 2 decimal places Amount of base required = theoretical amount – displaced amount   Where; Displaced amount = (amount of drug in g/DV of drug).

QUESTION 7 You are trying to use qPCR to compare gene expression of nitrous oxide reductase (nosZ) in a Pseudomonas sample treated under various conditions. Using the 2-delta deltact method, about how much higher (or lower) is the expression of nitrous oxide reductase (nosZ) in the experimentally treated sample verses the untreated sample? nosZ Ct values for untreated sample (control conditions) Internal housekeeping nosz Ct values for Internal housekeeping gene Ct values for untreated sample (control conditions) gene Ct values for treatment sample experimental treatment sample experimental Replicate 1 21.3 20.0 19.8 20.9 Replicate 2 21.5 20.1 19.7 21.0 Replicate 3 21.4 20.3 19.5 20.7 Replicate 4 21.6 20.2 19.4 21.1 O A. -2 times higher B. -100 times higher C. about 20% as much as the control O D.-3.5 times higher O E. -12 times higher O F. -6 times higher than the control

question 4

Question 4 Temoporfin (Foscan) is a photosensitizer used in photodynamic therapy for the treatment of squamous cell carcinoma of the head and neck. The prodrug is not being activated by internal enzymes but activated by light as an external stimulus. What is the benefit of this design? O To achieve spatiotemporal control of the killing of cancer cells. O More bioorthogonal as it does not introduce addition chemicals into the body. O To only target superficial cancer cells within the penetration depth of light. O More convenient for patients as they can activate foscan by staying in the sun.

Question: 1.  Atrial flutter For this disease pathology, please provide the following information: What causes this disorder (pathology)? Are there any threats to life risks associated with this disease that you should watch out for, and why? (Not all terms will have life-threatening conditions associated with them) What types of lab work or diagnostic testing (ECG, X-ray, ultrasound, MRI, etc.) would you typically see ordered with this disease? What would you expect to see in the lab or diagnostic results? What are the most common treatments? Which treatments would be the most important or take priority?

QUESTION 10 Six types of mammalian skulls were found throughout the owl pellet dissection lab. The data below is based on two years of collection. The data in a table and the graph, with missing labels, are shown below. Looking at the graph, identify what A through E should be labelled. Use the categories in the table to assist you with your labelling. Number of Skulls Collected Per Year Skull Type 2019 2020 Voles 80 98 Rats 16 20 Mice 130 157 Pocket Gophers 28 34 Shrews 25 18 Moles 55 45 D: 180 160 140 120 100 80 60 40 20 0. Voles Rats Mice Pocket Shrews Moles Gophers E: B C For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac).

Question number 2 & 3