For your first experiment you make a membrane of pure myristic acid and measure the melting curve (shown below). Label the position on the plot that indicates the melting temperature (Tm). In the space below describe the physical basis for the observed temperature response of the membrane. Membrane Fluidity Also draw the melting curve for stearic acid membrane Temperature
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- Assume that a membrane permeable to Na+ but not to Cl- separates two solutions. The concentration of sodium chloride on side 1 is higher than on side 2. Which of the following ionic movements would occur? a. Na+ would move until its concentration gradient is dissipated (until the concentration of Na+ on side 2 is the same as the concentration of Na+ on side 1). b. Cl- would move down its concentration gradient from side 1 to side 2. c. A membrane potential, negative on side 1, would develop. d. A membrane potential, positive on side 1, would develop. e. None of the preceding is correct.A student put together the experimental setup up shown. The selectively permeable membrane is permeable to both types of solute molecules shown. A. Describe the movement of the C molecules on side A of the apparatus shown. What will happen to these molecules over time? B. Describe the movement of the D molecules on side A. What will happen to these molecules over time? C. Explain the following: Once equilibrium is reached in the apparatus shown, will the molecules continue to move? Explain your answer. Selectively permeable membrane Side A Side B 00 • Molecule C o Molecule DTo which in is the membrane most permeable? You should have learned several basic principles of electrophysiology. Describe the key principle that your answer is based on [Na+]=10mM [K+]=100mM [Na+]=100mM [K+]=10mM Vm= -60mV
- Calculate ΔGinward. Is energy required for transport to happen? The cell is at 25°C. Membrane potential = -60 mV. What is the ΔGinward for chloride? Use the chart.Consider a solute having a permeability coefficient of 10-6 m s-1 for the plasma membrane of a cylindrical Chara cell that is 100 mm long and 1 mm in diameter. Assume that its concentration remains essentially uniform within the cell. Untitled Title A. How much time would it take for 90% of the solute to diffuse out into a large external solution initially devoid of that substance?* B. How much time would it take if diffusion occurred only at the two ends of the cell?* C. How would the times calculated in A and B change for 99% of the solute to diffuse out? D. How would the times change if Pj were 10-8 m s-1?*For most neurons, the extracellular concentration of chloride ions (Cl-) is 108 mM, whilethe intracellular concentration of Cl- is 5 mM.If the plasma membrane becomes more permeable to Cl-, would there be Clinflux or Cl- efflux at an RMP of -70 mV? Why?
- Two to three drops of mouse blood samples were placed in three different vials containing 0.07 M NaCl,0.15 M NaCl, and 0.30 M NaCl. A drop from each of the three vials were obtained and put on a slide forobservation under HPO. The effects of the different osmotic concentrations on the cells are shown in figures in your worksheets. Label the cell membrane for each figure. Give a short description (size and cell shape) for each of the RBC samples on the space provided in your worksheet. Compare their appearances with RBCs in the blood smear. Use the following guide questions in providing descriptions for each item. Which preparation has cells that look similar as those in the blood smear? What does this indicate about the movement of water in the cells? In which solution do the cells appear differently from the normal RBCs? What part of the cell could have possibly controlled such movement of water? What is its property that allowed this movement?It is typically sufficient to rupture cells when the solute concentration is reduced from 0.15M to 0.001M. Calculate what transmembrane pressure this would result in. Use that to access if the red blood cells would break. Yes or No? Compare to the transmembrane pressure when cells are in normal saline solution (0.91%NaCl) -> 0.156M(change unit to osM) Basically Calculate the transmembrane pressure when the solute concentration is reduced from 0.15M to 0.001M Determine if that transmembrane pressure would result in the breakage of red blood cells Calculate the transmembrane pressure when cells are in a normal saline solution and compareThe distribution of water is determined by solute concentrations. A hypertonic solution would cause a body cell to shrink in size. Both statements are true. Both statements are false. The first statement is true and the second statement is false. The first statement is false and the second statement is true. Spaced practice question: Consider a membrane protein and its chemistry. Which of the following would describe a channel protein? It is an inorganic compound. It is an example of a globular (functional) protein. It is stable when exposed to an acidic environment. It is an example of a fibrous (structural) protein.
- Uniporters and ion channels support facilitated transport across cellular membranes. Although both are examples of facilitated transport, the rates of ion movement via an ion channel are roughly 104 - to 105 -fold faster than the rates of molecule movement via a uniporter. What key mechanisticdifference results in this large difference in transport rate?What contribution to free energy (ΔG) determines the direction of transport?if an object b has a plasma sodium concentration of 135mOsm/L and an intracellular concentration of 4mOsm/L. It also has a plasma concentration of potassium of 20mOsm/L and an intracellular concentration of 200mOsm/L. studies identify that the cells have a permeability to potassium that is 10 times greater than sodium. What is the resting membrane potentialSuppose that the concentration of CI outside the cell is 100 and inside the cell is 10 mmol/liter. The Nernst equation at 20°C is: Eton = 58 millivolts/z- [10810 (m)] [lonlin You set the membrane voltage at 0 millivolts using a voltage clamp, and measure membrane current. If Cl is the only ion crossing the membrane, you would expect to see: Onegative charges flowing into the cell negative charges flow out of the cell 0 current the membrane hyperpolarizes (becomes more negative)