Looking ahead4. IPTG is an analog of lactose which is used to release the repressor on the lac operonallowing our protein to be produced.a. Draw IPTG and lactose and indicate the portions that are the same for bothstructures.b. Propose a reason that IPTG is used instead of simply adding lactose.

It can be defined as any alteration in a particular gene which gives rise to a different expression…

Answered: 4. IPTG is an analog of lactose which…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

2. Assume you have identified a new operon in bacteria (which you call the suc operon) that encodes enzymes required for catabolism of the sugar sucrose. The structural genes that are required to breakdown sucrose are transcribed from a promoter (Psuc). One of these enzymes is encoded by the sucS gene. Expression of the operon is controlled by a regulator, called SucR, which binds to an operator (O). Using your knowledge of the lac operon, interpret the results of the following experiments (Table 1) to propose a model describing how the sucrose operon might be regulated. Cell genotype Production of SucS enzyme Without sucrose With sucrose R* O* P* s* R O* p* s* ++ R* O P* S* -- RO* p* s* / R* Oʻ p* 5 R° O* p* S ++ -- ++ ++ Table 1. Production of SucS enzyme in various strains. “-" in the cell genotype indicates a loss of function mutation, “+" indicates wild type function, two genotypes separated by a / indicate a merodiploid or partial diploid. R° is a mutant version of SucR that…
Operons A. Draw a schematic of the lac operon when glucose is low and lactose is high in the cell’s environment. B. Draw a schematic of the trp operon when tryptophan levels in the environment are low. C. What is the lux operon and how is affected by quorum sensing?
1. Describe which enzymes are required for lactose and tryptophan metabolism in bacteria when lactose and tryptophan, respectively, are (a) present and (b) absent. 2. Contrast positive versus negative regulation of gene expression. Describe the role of the repressor in an inducible system and in a repressible system.
1. (a) Lactose levels are high Lac Operon Genes are switched OFF Lac Operon Genes are switched ON Does NOT affect the gene expression (b) Enhancer and activator proteins are working together Gene is switched ON Gene is switched OFF Does NOT affect the gene expression
6. In the space below draw the lactose operon in an environment that LACKS lactose. On your image label the following items: a. DNA b. Lac promoter C. Lac Z gene d. Lac Y gene e. Lac repressor protein f. Lac I gene g. Lac operator h. RNA polymerase i. mRNA message (if made)
3 5. Suppose you performed a B-galactosidase assay on E. coli with a l*P+O°Z*Y operon. What results would you expect in the absence and presence of IPTG? 6. If you did not get the expected results for the previous question, but you knew the genotype of the operon was correct, name two ways your experiment could have gone wrong.
1. Suppose there was a mutation in the inducer of the lac operon, such that the inducer was unable to function. How would the lac operon be affected? Please explain your reasoning in 1-3 sentences.
1. Please tell me: What's a mutation in moleculer terms? 2. When a mutation deletes a base in genomic DNA, how does it affect the expression product production and reading frame? 3. How does induction control enzyme synthesis using lactose operon?
1. Compare and contrast repressible and inducible operons. 2. Are genes for enzymes involved in anabolic processes usually repressible or inducible , why?
28. Which statement does NOT explain why the lac operon would not be effectively regulated by an attenuation mechanism like the trp operon? 1.Lactose is a substrate for the lac operon protein products. 2.Products of lac operon enzymes are not used in protein synthesis. 3.Transcription and translation do not happen simultaneously for the lac operon. 4.Attenuation is a suitable regulatory mechanism for anabolic amino acid pathways. 5.All of these statements explain why regulation by attenuation would not be effective for the lac operon.
In the ara operon in E. coli cells, AraC will bind to its specific target DNA when_ O a. when there is no glucose in cells. O b. it does not bind arabinose O c. it binds arabinose O d. it binds allolactose.
Explain the steps in Lac Operon. Take note, important enzymes and chemicals are needed in your explanation.

SEE MORE QUESTIONS

Recommended textbooks for you

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781305961135

Author:

Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

Fundamentals of General, Organic, and Biological …

Biochemistry

ISBN:

9780134015187

Author:

John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson

Publisher:

PEARSON

SEE MORE TEXTBOOKS

4. IPTG is an analog of lactose which is used to release the repressor on the lac operon allowing our protein to be produced. a. Draw IPTG and lactose and indicate the portions that are the same for both structures. b. Propose a reason that IPTG is used instead of simply adding lactose.