Consider the table below. What is the TDT for E. coli at 70 C? Please include the unit.

Using a schematic diagram, summarize the following steps in preparing competent cells for transformation: Inoculate a single colony of E. coli into 5 ml LB broth and incubate overnight at 37°C with moderate shaking (250 rpm). Add 200 μl of the culture into 50 ml LB broth and incubate overnight at 37°C with moderate shaking (250 rpm) to an OD600 = 1.3 to 1.5. Aliquot culture into five 15-ml pre-chilled, conical tubes. Leave tube on ice 5 to 10 min. Centrifuge cells 7 min at 1,600 × g (3,000 rpm), 4°C. Pour off supernatant and resuspend each pellet in 10 ml ice-cold CaCl2 solution (50 mM CaCl2), perform resuspension very gently, and keep on ice. Centrifuge cells 5 min at 1,100 × g (2,500 rpm), 4°C. Discard supernatant and resuspend each pellet in 10 ml ice-cold CaCl2 solution. Keep resuspended on ice for 30 min. Centrifuge cells 5 min at 1,100 × g, 4°C. Discard supernatant and resuspend each pellet in 10 ml ice-cold CaCl2 solution. Dispense cells (250 μl) into pre-chilled, sterile…

Compare and contrast the following protein characterization techniques in terms of the principles governing their functions.    electrosrapy ionization mass spectrometry vs polyacrylamide gel electrophoresis

complete the table below

The Bradford reagent gives a linear response only from 0.1 mg/mL to 1.4 mg/mL of protein concentration. Would we be able to use an "absorbance vs. concentration" line graph for samples outside of the range indicated? Justify your response.

What is optical density (OD) and how is it measured? How is it related to the concentration of the analyte? And what is a colorimetric assay?

Explain the basis for identification using biochemical testing. – Again, discuss enzymatic pathways, how do we make enzymes in a cell, what is the blueprint for an enzyme? Please keep this answer to 2-3 sentences

Please answer the following questions?

Write down a detailed description about the formation of polyacrylamide gel, how it is used for protein separations and the process of electrophoresis in these gels.

Calculate the doubling time of a culture of E. coli that is started with 3x105 CFU/mL and grows to 4.6x108 CFU/mL in 3 hours. Be sure to use units in your answer.