Final State Initial State Ab-Ag Ab Ab Varying Ag Amounts of Ab-Ag Ab Ab Antigen Ag Ag Ag-Ab-Ag Ab Ab Ab-Ag Ag Ab Ab Ab Ag Membrane permeable to Ag only
Final State Initial State Ab-Ag Ab Ab Varying Ag Amounts of Ab-Ag Ab Ab Antigen Ag Ag Ag-Ab-Ag Ab Ab Ab-Ag Ag Ab Ab Ab Ag Membrane permeable to Ag only
Human Physiology: From Cells to Systems (MindTap Course List)
9th Edition
ISBN:9781285866932
Author:Lauralee Sherwood
Publisher:Lauralee Sherwood
Chapter3: The Plasma Membrane And Membrane Potential
Section: Chapter Questions
Problem 5UC
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Question
Biomedical Engineering:
An equilibrium dialysis experiment was performed to characterize the binding affinity of a mouse IgG or human IgG antibody for an antigen, GAD65. (The association constant for the monoclonal mouse IgG is 4.75 x 10^8 M-1 and for a human IgG is 1.3 x 10^10 M-1)
a- calculate the dissociation constant for each antibody and explain which antibody has a higher binding affinity for GAD65?
b-calculate the fraction of free Ab sites for each antibody for a For a GAD65 concentration of 0.7 nM
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