7. Consider the following plasmid (size 6700 bp), with restriction sites at the positions indicated: BamHI + 1 6700 bp 2800 3500 EcoRI BamHI Probe c) This gel is transferred to a membrane in a Southern blot experiment, and hybridised to a radioactively labelled 100 bp probe, which anneals to the plasmid DNA at the position indicated on the diagram above. Draw the autoradiograph profile you would expect to observe for the membrane, indicate the sizes of the bands on the blot.

7. Consider the following plasmid (size 6700 bp), with restriction sites at the positions indicated: BamHI + 1 6700 bp 2800 3500 EcoRI BamHI Probe c) This gel is transferred to a membrane in a Southern blot experiment, and hybridised to a radioactively labelled 100 bp probe, which anneals to the plasmid DNA at the position indicated on the diagram above. Draw the autoradiograph profile you would expect to observe for the membrane, indicate the sizes of the bands on the blot.

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter15: Genes And Proteins

Section: Chapter Questions

Problem 27CTQ: Transcribe and translate the following DNA sequence (nontemplate strand): 5'- ATGGCCGGTTATTAAGCA-3'

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