fa restriction endonuclease recognizes and cleaves a linear piece of DNA and Circular DNA at 8 distinet places, how many pieces will result? OA Nine and Eight fragments respectively OB. Both gerierate nine fragments O C. Ten and nine fragments respectively D. Both generate elght fragments

Identify the sites of lagging strand synthesis. Select all that are true. B A 5 O a. C O b. D O c. A O d. B

10. A picture of a replication bubble is shown below. でイトイOpイ TT 3 Primer 5'-CUU-3" is being used to replicate this picce of DNA. What strand this primer will anneal to: the upper or the lower? Write what product of RNA replication would be the first five nucleotides that had been added onto the primer by DNA pol. Mark the 5' and 3' ends. lin

Consider the following:  Restriction enzymes bind and cut between bases T and A.  Based on this, which of the following are not acceptable VNTR segments?  The DNA segment under consideration is  AGTTAC      GTTACA TCAATG       CAATGT

33. In genetic engineering, DNA pieces that are cleaved by restriction enzymes are connected through the use of a. polymerases Ob. ligases c. transferases Od. hydrolases

Match to their function from list.

4. A linear fragment of DNA is exposed to a digest containing the individual restriction enzymes HindIII and Smal and then with a combination of the two enzymes. According to the gel electrophoresis results, the fragments obtained are Linear DNA HindIII Smal cut with: DNA HindIII + Smal 5.0 fragments 2.5 (kb) 5.5 3.0 2.0 2.5 2.0 a. Draw a map of the restriction sites on this linear piece of DNA. b. The mixture of fragments produced by the combined enzymes was then digested with the enzyme EcoRI. The results are fragments that are 2.5 kb, 2.0 kb and 1.5 kb. Add the restriction site for EcoRI on the map you created above.

33.Choose the DNA nucleotide sequence that would be complementary to the following strand (note: pay attention to DIRECTION of replication): 5' GTAGTCA 3' a 3' UATUAGA 5' b. 5' TGACTAC 3' 3' ACGACTG 5' 5' UGACUAC 3'. 5.Using agarose gel electrophoresis to analyze DNA, short DNA travels the long DNA. a faster than slower than at the same speed as d. Question 6 Which type of bonds link the individual nucleotides together in DNA? Glycosidic bond Peptide bond Phosphodiester bond Electrostatic bond a e Hydrogen bond Question 7 A typical engineered plasmid vector often contains the following features except a A poly linker region Origin of replication A signal peptide An antibiotic resistant gene a. b. C. C. d.

Please explain each step in detail for the process shown in the picture attached.

B. 25mL of 1% Sodium Bicarbonate solution was created and the ph was taken. Whic value below represents the most accurate representation fo the pH of the solution? a. pH 8.5 b. pH 7.0 c. pH 6.5 8. Agarose gel electrophoresis was performed below. Lane 1 is a 100 base pair DNA ladder. 1 2 3 Divong 20dut arinat & bak A. Does Lane 2 or lane 3 show a larger DNA fragment? B. Given the Lane 1 DNA ladder sizes, what is the approximate size (in base pairs) of the amplicon in Lane 3?