cloned fragment of DNA was sequenced by using the dideoxy method. A part of the autoradiogram of the sequencing gel is represented below. Deduce the nucleotide sequence of the DNA nucleotide chain synthesized from the primer. Label the 5’ and 3’ ends.

Q: 5) PCR primers have a melting temperature (Tm) at which they become dissociated from the template…

A: A primer is a short strand of nucleotide molecules that serves as an initial point for the synthesis…

Q: Using the same template strand, transcribe the DNA: GCA TAG CAA TGC

A: Answer: Transcription : It is the process of transcribing DNA to RNA using the template strand of…

Q: purpose(s) of DNA extraction

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Q: 5. Describe the process of DNA sequence of interest detection, using these terms appropriately,…

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Q: explain the rationales of adding chemicals that can affect DNA stability in a polymerase chain…

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Q: ing the gel picture of the DNA ladder, show where your digestion of the paper cutting model showing…

A: DNA is a double stranded biomolecule. The DNA is usually digested by endonucleases and exonucleases…

Q: 1) You would like to clone a gene by PCR. The sequence of the two ends of the coding strand for the…

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Q: Which of the following alternative steps cannot be employed in the DNA extraction because it would…

A: You have asked multiple questions.  I will answer 1st question,  as allowed by guidelines.  Asked :…

Q: 6. Describe the process of DNA sequence of interest detection, using these term appropriately,…

A: *DNA sequencing is a technique used to find the exact sequence of bases like Adenine, Guanine…

Q: The proofreading function of DNA polymerase involves the recognitionof a ________ and the removal of…

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Q: Give 2 advantages of SPACER DNA SEQUENCES as regions in the genome/chromosome to target for DNA…

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Q: 7. You are tasked with amplifying a fragment of interest from a sample of genomic DNA by using PCR.…

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Q: What happened to the DNA at the different temperatures? How does Polymerase Chain Reaction exploit…

A: Note: As per Bartleby Guidelines For Remaining Answers Please Repost The Question. Introduction: The…

Q: 5. Below is an image of DNA sequenced using the Sanger sequencing method, where different…

A: Gel electrophoresis is a molecular technique that aims to separate the DNA, RNA, and protein…

Q: 3. Give a schematic diagram of the different steps involved in PCR (Polymerised chain reaction) of a…

A:

Q: 1. Given the following restriction endonucleases and the sequences of their corresponding…

A: Prokaryotic cells are unicellular organisms that differ from multicellular organisms in composition…

Q: 2. The restriction enzymes Xhol and Sall cut their specific sequences as shown below: Xhol 5'-c…

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Q: Which is the odd one out ? For the rest, explain the concept/process/technique they are involved…

A: DNA Polymerase is the main enzyme of replication. It adds deoxyribonucleotides according to the…

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Q: 9. Estimate the sizes of the bands, in each of the lanes for the gel attached below. Then, for each…

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Q: The proofreading function of DNA polymerase involves therecognition of a ________ and the removal of…

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Q: The diagram shows an autoradiograph of a DNA sequencing gel. What is the 5' to 3' sequence of the…

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Q: If we extract DNA of a pool of cells, will that impact the DNA sequencing? In other words, having…

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Q: 4. A linear fragment of DNA is exposed to a digest containing the individual restriction enzymes…

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Q: 22. Which statement is correct about restriction enzyme? O They cut methylatedsite O They cut…

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Q: 8. Describe the possible outcome of a PCR experiment in which (a) one of the primers is…

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Q: 9. Five DNA samples are shown along with their recognition sites where a particular restriction…

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Q: 36. Which is the sequence of steps used to amplify DNA with PCR during a single cycle? 1) Primers…

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Q: 3. Look at the sequences below. If you add a restriction enzyme(that cuts at GAATTC) to the uncut…

A: as per our company guidelines we are supposed to answer only first 3 sub-parts. Kindly repost other…

Q: 5. A scientist was studying a fragment of eukaryotic DNA that she suspected was involved in a…

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Q: How is a randomized DNA library created?   MARK ALL THAT APPLY. the use of Mn2+ rather than Mg2+ by…

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Q: 7. You are preparing a library to sequence two samples, one from a WS and one from a FS, via…

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Q: 2) Why was there a greater A260 absorbance reading for your DNA sample that was incubated at higher…

A: 2. Why was there a greater A260 absorbance reading for your DNA sample that was incubated at higher…

Q: A cloned fragment of DNA was sequenced by using thedideoxy chain-termination method. A part of the…

A: The process is usually defined as the way that they are been determining the sequence of nucleotides…

Q: Explain the importance of Isolating DNA from the cells of organisms.

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Q: Make simple steps to extract isolating DNA from strawberries and yeast.

A: Answer

Q: 1. Transcribe the DNA strand provided then determine the sequence of amino acids of the gene…

A:

Q: The diagram illustrating the polymerase chain reaction (PCR) technique is provided below. How does…

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Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.