F 400 800 pCi (μmol mol ¹) 1200 A. The function of phosphoglycerate kinase was studied in response to different CO2 levels (pCi). Pi5 and Pi3 indicate plants for which phosphoglycerate kinase was knocked out and WT has a fully functional phosphoglycerate kinase. Data are presented as the means ± SD (n = 3). * denotes significant differences in comparison with the values in wild-type plants versus the knockout (Pi5/Pi3) plants. Given this information, what is the role of CO2 and phosphoglycerate kinase in photosynthesis, as measured by rate of CO2 assimilation? B. If electron transport in the chloroplast was negatively altered, what would be the impact of that alteration on phosphoglycerate kinase? C. The delta G for the reaction that converts 3-phosphoglycerate to 1,3- bisphosphoglycerate is 0.09 kJ/mol. Please talk about the thermodynamics of this reaction and the role of phosphoglycerate kinase in the reaction.

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Ra
400
800
pCi (μmol mol ¹)
1200
A. The function of phosphoglycerate kinase was studied in response to different CO2
levels (pCi). Pi5 and Pi3 indicate plants for which phosphoglycerate kinase was
knocked out and WT has a fully functional phosphoglycerate kinase. Data are
presented as the means ± SD (n = 3). * denotes significant differences in
comparison with the values in wild-type plants versus the knockout (Pi5/Pi3)
plants. Given this information, what is the role of CO2 and phosphoglycerate
kinase in photosynthesis, as measured by rate of CO2 assimilation?
B. If electron transport in the chloroplast was negatively altered, what would be the
impact of that alteration on phosphoglycerate kinase?
C. The delta G for the reaction that converts 3-phosphoglycerate to 1,3-
bisphosphoglycerate is 0.09 kJ/mol. Please talk about the thermodynamics of this
reaction and the role of phosphoglycerate kinase in the reaction.
Transcribed Image Text:Ra 400 800 pCi (μmol mol ¹) 1200 A. The function of phosphoglycerate kinase was studied in response to different CO2 levels (pCi). Pi5 and Pi3 indicate plants for which phosphoglycerate kinase was knocked out and WT has a fully functional phosphoglycerate kinase. Data are presented as the means ± SD (n = 3). * denotes significant differences in comparison with the values in wild-type plants versus the knockout (Pi5/Pi3) plants. Given this information, what is the role of CO2 and phosphoglycerate kinase in photosynthesis, as measured by rate of CO2 assimilation? B. If electron transport in the chloroplast was negatively altered, what would be the impact of that alteration on phosphoglycerate kinase? C. The delta G for the reaction that converts 3-phosphoglycerate to 1,3- bisphosphoglycerate is 0.09 kJ/mol. Please talk about the thermodynamics of this reaction and the role of phosphoglycerate kinase in the reaction.
2. Sugar is made in the chloroplast through CO2 assimilation by the Calvin cycle. This
cycle involves 11 enzymes and produces carbohydrates from atmospheric CO2 using
NADPH and ATP supplied by the light reactions. Chloroplast phosphoglycerate kinase
(cpPGK) catalyzes the conversion of 3-phosphoglycerate to 1,3-bisphosphoglycerate
using ATP (step #2).
CHI-O-PO
C=0
Rate of CO₂ assimilation
(μmol CO₂ m² s¯¹)
H-C-OH
H-C-OH
6 ADP+6P, Transketolase
CH,-O-PO
6 Ribulose-1,5-bisphosphate
6 ATP
40
H
C=O
H-C-OH
CH,-O-PO
10 Glyceraldehyde-3-phosphate
30
20
6C0₂
10
Ribose phosphate isomerase
Phosphoribulokinase
To cytoplasm
C=O
H-C-OH
CH,-o-PO,
2 Glyceraldehyde-3-phosphate
CH,-O-PO2
HO-C-COO
C=0
H-C-OH
CH,-O-PO
6 Carboxylase intermediate
Ribulose
bisphosphate
carboxylase
1
CALVIN
CYCLE
H
C=O
H-C-OH
CH,-O-PO 2
12 Glyceraldehyde-3-phosphate
Sugars
Glyceraldehyde-3-phosphate
dehydrogenase
(3)
04 *
12 P
PEA
400
800
pCi (μmol mol ¹)
9
C=0
H-C-OH
Phosphoglycerate
kinase
2
CH,-O-PO
12 3-Phosphoglycerate
C-O-PO,²
H-C-OH
CH,-O-PO
12 1,3-Bisphosphoglycerate
12 NADPH
12 NADP+
WT
Pi5
Pi3
12 ATP
+12 ADP + 12 P
04 *
1200
Transcribed Image Text:2. Sugar is made in the chloroplast through CO2 assimilation by the Calvin cycle. This cycle involves 11 enzymes and produces carbohydrates from atmospheric CO2 using NADPH and ATP supplied by the light reactions. Chloroplast phosphoglycerate kinase (cpPGK) catalyzes the conversion of 3-phosphoglycerate to 1,3-bisphosphoglycerate using ATP (step #2). CHI-O-PO C=0 Rate of CO₂ assimilation (μmol CO₂ m² s¯¹) H-C-OH H-C-OH 6 ADP+6P, Transketolase CH,-O-PO 6 Ribulose-1,5-bisphosphate 6 ATP 40 H C=O H-C-OH CH,-O-PO 10 Glyceraldehyde-3-phosphate 30 20 6C0₂ 10 Ribose phosphate isomerase Phosphoribulokinase To cytoplasm C=O H-C-OH CH,-o-PO, 2 Glyceraldehyde-3-phosphate CH,-O-PO2 HO-C-COO C=0 H-C-OH CH,-O-PO 6 Carboxylase intermediate Ribulose bisphosphate carboxylase 1 CALVIN CYCLE H C=O H-C-OH CH,-O-PO 2 12 Glyceraldehyde-3-phosphate Sugars Glyceraldehyde-3-phosphate dehydrogenase (3) 04 * 12 P PEA 400 800 pCi (μmol mol ¹) 9 C=0 H-C-OH Phosphoglycerate kinase 2 CH,-O-PO 12 3-Phosphoglycerate C-O-PO,² H-C-OH CH,-O-PO 12 1,3-Bisphosphoglycerate 12 NADPH 12 NADP+ WT Pi5 Pi3 12 ATP +12 ADP + 12 P 04 * 1200
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