Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Transcribed Image Text:**Ibuprofen and Indomethacin as Inhibitors of Prostaglandin H₂ Synthase-1: Experimental Analysis**
Ibuprofen and indomethacin are clinically important inhibitors of the enzyme prostaglandin H₂ synthase-1. In this study, cells expressing this enzyme were incubated under four different conditions. The activity level of the enzyme was subsequently measured by adding radiolabeled arachidonic acid and detecting newly-produced prostaglandin H₂. The incubation conditions were as follows:
1. 40 minutes without inhibitor (control).
2. 40 minutes with the inhibitor.
3. 40 minutes with the inhibitor, followed by resuspension in a medium without the inhibitor.
4. 40 minutes with the inhibitor, resuspension in a medium without the inhibitor, followed by an additional 30 minutes of incubation.
**Graphical Analysis:**
*Ibuprofen:*
- The bar graph depicts the results for ibuprofen across the four incubation conditions.
- Condition 1 (control) shows a high level of prostaglandin H₂ synthesis, set as 100% relative to the control.
- Condition 2 exhibits a significant drop in synthesis activity, indicating effective inhibition.
- In condition 3, the synthesis recovers to nearly the same level as the control after resuspension without the inhibitor.
- Condition 4 shows restored synthesis similar to or slightly higher than the control.
*Indomethacin:*
- Similarly, the bar graph for indomethacin reflects synthesis across the four conditions.
- Condition 1 maintains a high synthesis rate (100%), like the control.
- Condition 2 demonstrates a marked decrease in enzyme activity, confirming inhibition by indomethacin.
- Conditions 3 and 4 result in low activity, indicating less recovery of synthesis upon resuspension, compared to ibuprofen.
These graphs illustrate the varying efficacy and reversibility of inhibition by ibuprofen and indomethacin under different experimental conditions.
Transcribed Image Text:**Question:**
Which hypothesis best explains the results for these two inhibitors?
- ( ) Ibuprofen and indomethacin both covalently modify prostaglandin H₂ synthase-1.
- ( ) Ibuprofen dissociates slowly from prostaglandin H₂ synthase-1, whereas indomethacin dissociates rapidly.
- ( ) Ibuprofen dissociates rapidly from prostaglandin H₂ synthase-1, whereas indomethacin dissociates slowly.
- ( ) Ibuprofen and indomethacin both dissociate rapidly from prostaglandin H₂ synthase-1.
**Activity:**
How would these results look if aspirin were tested in a similar fashion? Complete the bar graph by dragging a bar to each column. Some bars may be used more than once and some may not be used at all.
**Graph Explanation:**
The bar graph is titled "Aspirin" and measures "Prostaglandin H₂ synthesized (relative to control)" on the vertical axis, with values labeled at intervals: 0, 20, 40, 60, 80, and 100. The horizontal axis features four columns numbered (1), (2), (3), and (4).
To the right, there is an "Answer Bank" consisting of four green bars of varying heights:
1. Tall bar reaching 100.
2. Medium bar reaching about 40.
3. Short bar reaching about 20.
4. Very short bar reaching about 10.
Participants are tasked with placing the bars from the "Answer Bank" into the corresponding numbered columns in a way that reflects potential results for aspirin under similar test conditions.
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