Explain how to prepare 250 ml liquid and 500 ml solid form separately from LB (Luria Bertani) medium whose components and concentrations are given below. Yeast extract 5 g/L Peptone 10 g/L NaCl 10 g/L Agar 1.5 g/L

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Q: Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL…

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Q: Why does motility media contain a lower amount of agar than ordinary agar?

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Q: Explain why a green metallic sheen is formed when E. coli is grown on Eosin Methylene Blue Agar.

A: Escherichia coli is the gram negative bacteria.

Q: What nutrients do the following media components contain? • Peptone Yeast extract Beef extract •…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Determine the amount of dehydrated medium needed to prepare 50 nutrient agar plates. Include amount…

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Q: Explain why litmus milk is considered a good differential medium.

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Q: Why would the following medium not be considereda chemically defined medium: glucose, 5 grams…

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A: In microbiology, pure culture, a laboratory culture which contains one organism species. A pure crop…

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Q: State four reasons why barley is terminated in beer brewing process.

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Q: Provide comparison and illustration of expected growth curves from a pure culture with the…

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Q: Why is PBS buffer preferred when preparing a monolayer cell culture?

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Q: Compare and contrast use dilution and disk diffusion methods of evaluating disinfectants. Please…

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Q: give one reason why is it necessary to keep stirring the agar medium mixture in a beaker when…

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Q: During media preparation, you observed that the agar medium did not solidify after sterilization…

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Q: Graph of Absorbance vs Time(mins) 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 10 20 30 40 50 60 70 80 Time…

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Q: What is differential medium

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Q: What is the purpose of adding sodium bicarbonate to a culture medium?

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Q: A culture consisting of 100 litres of nutrient medium containing 12 g l-1 of growth limiting…

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Q: Which stationary phase is better in separation of the components of moringa extract for column…

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Q: Why is a GasPak anaerobic jar necessary for the culture of anaerobes on Brewer’s anaerobic agar…

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Q: OCULAR ОBJECTIVE TOTAL MAGNIFICATION 10x 1. 100x 10х 2. 400x 10х 100x 3. 10x 5x 4.

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Q: There are 5 TSIA media A-E beginning from the left. Describe the reactions in tubes B, C, D, E only.

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Q: In determination of microbial motility, what is the value of a hanging-drop perparation? what is the…

A: Hanging drop is a motility testing which helps microbiologist to view motility of bacterium.

Q: Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL…

A: Serial Dilution -- Serial dilution are used to calculate the concentration of microorganism .It…

Q: A culture of S. cerevisea has an overnight OD of 2.3 (1.0 OD is approx 1.0x107 cells/ml) You will be…

A: Dilution is the process of reducing the concentration of a solute in a solution. This is typically…

Q: (b) A Food material containing Bacillus stearothermophilus PS1518 as an indicator organism ts…

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