Explain how to prepare 250 ml liquid and 500 ml solid form separately from LB (Luria Bertani) medium whose components and concentrations are given below. Yeast extract 5 g/L Peptone 10 g/L NaCl 10 g/L Agar 1.5 g/L

There are 5 TSIA media A-E beginning from the left. Describe the reactions in tubes B, C, D, E only.

A culture of S. cerevisea has an overnight OD of 2.3 (1.0 OD is approx 1.0x107 cells/ml) You will be plating 100µl onto agar and want the final count of colonies on the plate to be around 300 colonies.  How much of the 2.3 OD culture must you use to get a 500µl subdilution (with sterile water), so that you have diluted enough to get approx 300 colonies per 100ul

A lab interested in isolating Escherichia coli from food samples uses a lot of Eosin Methylene Blue (EMB) Agar. The recipe for this media is 36 g/L deionized water. How many grams of media would be required to prepare 350 plates assuming 20 mL per plate? 072 O 112 O 90 O 252

250 uL of 1M azide was added to a 25 mL yeast solution. What was the concentration of azide in the yeast culture? (use proper units)

Below is an image of 3 different bacteria, letter labeld, growing on MacConkey Agar. Note that bacteria such as E. coli use mixed acid fermentation, with very high acid production which will cause a colored precipitation around colonies on MacConkey agar. MacConkey Agar C

Calculate the GI50 value from the graph showing different concentrations of N3P. Show full working. Explain if cytotoxicity is caused by test agent.

Create a flow chart to perform the required tests to identify unknown microorganism: - Use at least 3 other biochemical tests from the following list: Mannitol salt agar, Blood agar, Starch agar, Tributyrin Agar, Gelatin, Casein agar, Indole Production, MR-VP, Citrate, Hydrogen Sulfide test, Urease test, Nitrate reduction test, Catalase test, and Oxidase test. The Gram positive organisms that could be present in your unknown mixture are: Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis and Lactococcus lactis.

Given the following media recipe, pick the terms that apply (pick as many as are applicable). 10.0 g Meat extract 10.0 g Peptone 5.0 g Sodium chloride 15.0 g 1.0 L Agar Water Once media is cool, add 6% sterile sheep's blood Defined Complex Minimal Selective Differential

A culture of S. cerevisea has an overnight OD of 4.5 (1.0 OD is approx 1.0x107 cells/ml) You will be plating 100µl onto agar and want the final count of colonies on the plate to be around 300 colonies.  How much of the 4.5 OD culture must you use to get a 500µl subdilution (with sterile water), so that you have diluted enough to get approx 300 colonies per 100ul