Explain how to prepare 250 ml liquid and 500 ml solid form separately from LB (Luria Bertani) medium whose components and concentrations are given below. Yeast extract 5 g/L Peptone 10 g/L NaCl 10 g/L Agar 1.5 g/L

Q: What is the result for the yellow tube of Methyl red test? What must the pH be in each tube? What is…

A: Methyl red test This test usually aims to differentiate the facultative anaerobes enteric bacteria.…

Q: The following is a picture of Eosin Methylene Blue Agar. What does the growth on this plate…

A: Eosin Methylene Blue (EMB) agar is a selective and differential culture medium which selectively…

Q: Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL…

A: Serial dilution is a progression of successive dilutions used to lessen a thick culture of cells to…

Q: My question is: Could you explain steps? (etc. why do we heat or add chemicals like nacl or ammonium…

A: Casein is the major protein component found in milk. It is generally a phosphoprotein which is…

Q: Write the materials,  reagents, Principle and  procedure for the Periodic acid Schiff technique

A: Answer: STAINING = It is the method to colorize the smear or transparent tissue or cells in order to…

Q: This is the growth curve for Clostirdium (incubated under optimal growth conditions). Use it to…

A: There are 5 phases to a logistic (sigmoidal) growth curve.  They are stationary phase, lag phase,…

Q: What are peptones, yeast extract, beef extract, thioglycollate, and agar? Why are they used in…

A: A suitable nutrient medium that provide appropriate biochemical environment and maintains all the…

Q: ium for the Cultivation of Saccharomyces cerevisid Component Value Unit g/L g/L lucose 5 east…

A: Algae are a diverse group of aquatic organisms with specific characteristics with the  ability to…

Q: 300 +0,005 mg/mL - 0,010 mg/mL - 0,015 mg/mL +0,020 mg/mL 0,025 mg/mL + 0,030 mg/mL -0,035 mg/mL 250…

A: Microbial Growth Curve: The number of living cells in a bacterial population over time is…

Q: Is the eosin methylene blue agar? (EMB) a complex or defined medium? Explain based on its…

A: Complex media consists of nutrients such as water soluble extracts of plant or animal tissue and…

Q: What nutrients do the following media components contain? • Peptone Yeast extract Beef extract •…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Choose the one answer that fits best. Which of the following statements regarding the proper…

A: Micropipettes are used in the laboratory to transfer very little quantity of liquid. Parts of Micro…

Q: Can you classify the following biochemical tests based on the following criteria and explain why you…

A: Different bacteria survives on different biochemical reactions. These biochemical reactions are used…

Q: Lactose fermentation

A: The dichotomous key is the tool for the identification of unknown organism among the group. Choice…

Q: Why it is possible/ applicable to use recrystallization and vacuum filtration with Luminol? Cite…

A: Luminol is a chemical used in many laboratory experiments, like in biotech research lab, forensic…

Q: The optimum growth pH for a microorganism is usually:  4,5,7 or 8

A: pH is the scale on which the acidity or alkalinity  is measured. It is the scale between 1 to 14.…

Q: As a public health engieer you have been tasked wiyh designing a plug flow reactor for the…

A: The Surface Water Treatment Rule, or SWTR for short, is an EPA regulation that establishes drinking…

Q: do methodologies preparation of the following 6 solutions (Broth LB, LB Agar, Potato-Dextrose Agar,…

A: A gel or liquid that contains nutrients and is used to cultivate bacteria or other microorganisms is…

Q: You are given reference temperature 121 °C, Z value 10 °C, F0 15 min for killing Clostridium…

A: Since the heat treatment of the food kills all microorganisms that might ruin the food and those…

Q: Questions 1 Predict the effect of the following "mistakes" made when performing a Gram stain. Your…

A: In order to understand the mistakes made in Gram staining procedure we need to understand the whole…

Q: Match inhibitors, selective ingredients, pH indicators and other components to correct medium phenol…

A: Cultured bacteria are any bacteria that grow in the lab by the microbiologist using the required or…

Q: in a clean, non-sterile 15 mL centrifuge tube, prepare a 2.0% yeast suspension by adding 0.06 g…

A: Introduction  Dilution is the process of reducing the concentration of a solution by adding a…

Q: Before starting the freeze-drying process, the weight of the test tube, Wi which is 15.33g and test…

A: Given, Weight of the test tube is 15.33g Test tube weight with microbial cell pellets is 34.94g…

Q: What is the EFFECT and RATIONAL of Molten agar mixed with bacterial suspension has temperature…

A: Bacteria can be cultured in either a liquid media or a solid media based on the purpose of culturing…

Q: All recipes are given per litre unless specified.   Lambda Broth (LB) Plates…

A: Give, The final volume (V2) = 5mL. The stock concentration of MgSO4 (C1)= 1M The desired…

Q: What nutrients do the following media components contain?   Peptone   Yeast extract   Beef…

A: “Since you have posted a question with multiple sub-parts, we will solve the first three sub-parts…

Q: You have 0.05 ml of kombucha that contains microbes at a concentration of 8.2 x 10^6 CFU/ml. You…

A: Creating proper dilutions and working out the colony-forming units or CFU/ ml is one integral part…

Q: What would you need to culture the following microorganisms? (Note the hints and/or choices after…

A: Thermophile - Organisms tolerant to high temperature  Mesophile - organisms tolerant to moderate…

Q: Sulfur Indole Motility (SIM) Medium H2S produced, color and +/-: ______________________________…

A: Sulphur indole motility medium used to test the ability of am organism to have properties of…

Q: State four reasons why barley is terminated in beer brewing process.

A: Beer brewing:         The process of production of beer by steeping a starch source in water,…

Q: What is differential medium and differential media? Explain the difference ?

A: Difference between differential medumium -   .- A differential medium support the expansion of any…

Q: Using pearson's square, how to attain 30o Brix syrup using  50oBrix and 20o Brix syrup solutions?

A: Here, the given two available syrups are 50°Brix and 20°Brix, that is one of the solutions is higher…

Q: How to calculate for FAME yield given the dried FAME extracted of 0.021 g and the volume of culture…

A: Fatty acid methyl esters or FAME are a type of fatty acid esters. This is formed by the…

Q: Identify the oxidase-negative, gram negative bacillus isolated from the blood cultures of a…

A: Gram-negative bacteria are those that do not retain the crystal violet stain after gram staining.…

Q: What kind of method of preparation was done? method of preparation (e.g. decoction, infusion,…

A: Given information The extracts are taken from the leaf and stem of M. quadrifolia and soaked in…

Q: What medium is this? What is the result in tube at left? In second tube? What does it tell you about…

A: One stab towards the bottom of the SIM tube is used to inoculate it. If the microorganism is motile,…

Q: chemical(s) best at inhibiting both Gram- negative and Gram-positive bacteria [ Choose ] chemical…

A: Bacteria are microscopic, unicellular, prokaryotic organisms found in diverse environments. They can…

Q: How could calculate this serial dilutions probelms for microbiology and how do can I know what tube…

A: Serial dilution is a method of step-wise dilution of bacterial culture to reduce the concentration…

Q: draw a schematic diagram on the Extraction of Flavonoids using the procedures stated below.

A: Extraction of flavonoids

Q: a. Let’s say for example that a milk sample has 10,000 bacteria per milliliters. If 1 mL of this…

A: A serial dilution is a process of sequential dilution in order to decrease the density of cell…

Q: Appearance Motility observed Staphylococcus epidermidis Escherichia coli of 1180 C e

A: Staphylococcus epidermidis is a gram-positive microorganism that is commonly found in the human…