Sulfur Indole Motility (SIM) Medium H2S produced, color and +/-: ______________________________ Indole present/Tryptophan hydrolysis, color and +/-: ___________________________ Motile or non-motile: _____________________________

Q: Name of Organism: Escherichia coli Туре of Medium Positivel Color Before Incubation Color After…

A: Escherichia Coli is a Gram-negative anaerobic, rod-shaped bacteria, found abundant in the lower…

Q: TUBE NO. 1. 3 ml of RESULT and OBSERVATION TREATMENT | 2 mL of NAOH solution egg albumin solution…

A: Proteins are macromolecules that are made up of Amino acids joined by peptide bonds. Linear chain of…

Q: Briefly explain this statement "Antineoplastic natural products- Taxol"

A: Taxol is an enemy of malignancy ("antineoplastic" or"cytotoxic") chemotherapy drug. Taxol is…

Q: Why do cells need to be kept in the freezing devices first then transferred to liquid nitrogen…

A: Rapid freezing of cells can cause ice crystals large crystals can be formed in which cells are…

Q: Why does motility media contain a lower amount of agar than ordinary agar?

A: Agar is a phyco-colloid polymer of galactose monomers obtained from red algae (Gelidium and…

Q: Explain why a green metallic sheen is formed when E. coli is grown on Eosin Methylene Blue Agar.

A: Escherichia coli is the gram negative bacteria.

Q: 300 +0,005 mg/mL - 0,010 mg/mL - 0,015 mg/mL +0,020 mg/mL 0,025 mg/mL + 0,030 mg/mL -0,035 mg/mL 250…

A: Microbial Growth Curve: The number of living cells in a bacterial population over time is…

Q: Sydney Brenner isolated Salmonella typhimurium mutants that were implicated in the biosynthesis of…

A: Beadle and Tatum gave the concept of one gene one enzyme hypothesis according to which a particular…

Q: FOOD MICROSCOPY: STARCH IDENTIFICATION  The following figures are the photomicrographs of the…

A: Starch is mainly found in a plant source, as starch is a storage material.

Q: Select one: a. continuous, batch, fermenter b. batch, continuous, chemostat c. batch, continuous,…

A: Microbial fermentation can be carried out as either a once off ----------------- culture or…

Q: Why would the following medium not be considereda chemically defined medium: glucose, 5 grams…

A: A chemically defined media is a medium that has all the ingredients of known quantities.…

Q: how do microorganisms such as bacteria eliminate harmful reactive oxygen species?

A: Reactive oxygen species (ROS) are emerging as important elements in the bacterial response to lethal…

Q: A mixture of dipeptides consisting of Lys-Gly, Thr-His, Ser-Leu, Glu-Gln was applied to column…

A: Ion-exchange chromatography is a type of protein purification technique in which proteins are…

Q: where 5 nutrient agar pates per female student and 3 agar slants per males student are needed to…

A: It is given that 5 nutrient agar plate prepared by per female so Seven female will prepare=7*5=35…

Q: Type of Medium Original Color Any Chemicals Added to Visualize Reaction? Negative Result Positive…

A: Phenol red Glucose original colour will be yellow.  Negative results indicates that there is drop in…

Q: Lysine decarboxylase media is yellow after incubating an organism in it at 37 degrees for 24 hours.…

A: Lysine decarboxylase is an enzyme that is used in decarboxylation test. It is used to distinguish…

Q: Lysozyme and benzonase are included in the lysis buffer, given that these are enzymes, one might not…

A: Lysozyme and Benzonase are enzyme and Endonuclease respectively.

Q: Nitrate reduction to nitrite is indicated by the formation of a pink to red colour after addition of…

A: Sulfanilic acid reacts with nitrous acid to form diazotized sulfanilic acid if introduced. This…

Q: A plant isolate was subjected to qualitative tests to determine the presence of macromolecules.…

A: Plant isolates are obtained from different tissue homogenate samples of plant parts. Usually, a…

Q: Proteus vulgaris is positive for the production of the enzyme phenylalanine whereas Escherichia coli…

A: INTRODUCTION Proteus vulgaris is a Gram-negative, rod-shaped, nitrate-reducing, indole-positive, and…

Q: Nutrient Agar (NA) is a general purpose medium used for the cultivation of a wide variety of non-…

A: Nutrients are the compounds found in food that give us energy, allowing us to heal and develop,…

Q: EXPERIMENT : REMOVAL OF WATER FROM MICROBIAL CELLS USING FREEZE  DRYING METHOD Objective To learn…

A: Freeze drying is a method that involves placing a completely frozen sample under a vacuum to remove…

Q: If a component is heat sensitive, how might you conveniently achieve sterility of that medium? a.…

A: Option A i.e. filtration

Q: Medium used for cellulase production contained (g/L): NaNO3, 3; MgSO4 7H2O, 0.5; K2HPO4, 1; KCl, 0.5…

A: Culture media is proper nutrient for the growth of the microorganisms, which help to grow a variety…

Q: If a sample is composed of a mixture of maltose, maltodextrin, and glucose, which of the following…

A: Sugars capable of reducing ferric or cupric ions are called reducing sugars. All monosaccharides…

Q: A certain medium has the following composition: Glucose                                   15 g…

A: Culture media are used to provide the mandatory nutrients to the microorganisms for their growth and…

Q: If you have a 15 mg/100 ml stock solution of GA3 and you need a 1 mg GA3 in 25 ml, how much stock…

A: Given: volume of stock solution is 15 mg/100 ml Needed amount is 1 mg kinetin in 25 ml Volume of…

Q: What is true about 'cracking' of emulsion? Select one: O a. The primary emulsion does not become…

A: Emulsion can be defined as the mixing of two or more liquids which are not immiscible (nonmixable or…

Q: Media - Possible Results Туре of Medium Any Chemicals Added to Visualize Reaction? Original Color…

A: Disclaimer: According to the Bartleby guidelines the first table is completed. The rest of the parts…

Q: Why is a GasPak anaerobic jar necessary for the culture of anaerobes on Brewer’s anaerobic agar…

A: Brewer’s anaerobic agar and fluid thioglycollate medium (FTM) are used to grow bacteria. Both of…

Q: Which of the following samples are expected to cause a decrease in absorbance over time when…

A: Ensuring adequate light reaching a detector by using water colloidal solutions in light or X-ray…

Q: A photo-sensitive organic reagent is exposed to 50 milliWatt ruby red laser light (694

A:

Q: If the volume of a Staphylococcus aureus cell is estimated at 0.5 μm3, how many cells could be…

A: Staphylococcus aureus is a gram-positive bacteria, the cell wall is made up of a dense peptidoglycan…

Q: A certain medium has the following composition: Glucose                15 g Yeast extract      5 g…

A: Since you have asked as question with multiple subparts, we will answer only first three subparts…

Q: Please help me answer how much volume of cell suspension needed (for 5*10^4 cells) in microliters.…

A: For some experiments, it is essential to use the specified number of cells of microbes (or…

Q: If you mix heated pepsu) enzyme to abont 39 C with allbunin, then add biuret reagent, the expected…

A: Biuret test is a colorimetric method used to carryout in laboratory to determine the protein…

Q: Why is it necessary to store the molten agar in a water bath at 50 degrees Celsius?

A: Introduction:-  Agar is made up of two polysaccharides: agarose and agaropectin, with agarose…

Q: ph Minimum Optimum Maximum Escherichia coli 4.5 7 9 Pseudomonas aureus 4.5 7.5 9.5 Staphylococcus…

A: The change in the number of live cells in the population of bacteria over the period is…

Q: A strawberry juice containing 5x10 bacteria cells/ml is pasteurized at 80°C for 30s. The average…

A: Established industry practiceThere is an established practice among fruit juice manufacturers and…

Q: • Analysis that compares the results obtained from the growth of S. thermophilus under varying…

A: Streptococcus thermophilus is a gram positive bacterium that is found in fermented milk. It plays a…

Q: Which step is catalyzed by the enzyme responsible for the Met 2 mutant? (picture attached) A)…

A: Synthesis of the amino acid methionine involves four genes in Neurospora. Methionine mutants cannot…

Q: above are correct Except: All of the following are correct regarding the disk diffusion test photos…

A: Bacteria are microscopic, single-celled organisms that exist in every environment. They are present…

Q: Name at least one reason that we might want to use a Winogradsky column rather than a laboratory…

A: The Winogradsky column can be used to study various levels of growth that is aerobic or anaerobic…

Q: Why are plated media stored in an inverted position?

A: Plated media enables the isolation of microorganisms from samples or specimens.

Q: You are cultivating Escherichia coli in a chemostat culture. The maximum specific growth rate (µmax)…

A: A chemostat is a bioreactor containing culture where the fresh medium is continuously introduced at…

Q: Why is sulfate (SO42-) added to a Winogradsky column?

A: Winogradsky developed the columns as a means of enriching sediments and soils for microbes.…

Q: C. Chemical Nature of Polyphenoloxidase Enzyme Activity (Color Intensity) Light pink (Moderate…

A: Biochemical reactions that occur in the living cells are catalyzed by enzymes.

Q: How is fluorescence spectroscopy beneficial for pharmacological analysis ? Please shortly answer at…

A: Fluorescence spectroscopy uses a beam of light that excites the electrons that are present in the…

Q: When you did Wright-Giemsa staining, you added the blue dye in the well containing C2C12 cells. The…

A: Lyophilization is a process of freeze-drying the sample such as cells and tissues under a vacuum…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.