Match inhibitors, selective ingredients, pH indicators and other components to correct medium phenol red pH indicator [ Choose ] [Choose] mannitol Mac Sabouraud Agar 7.5 g NaCI/100 ml inhibitor Blood agar MSA low pH, high dextrose concentration [ Choose ] inhibitors neutral red pH indicator [ Choose ] crystal violet and bile salt inhibitors [ Choose ] no inhibitors [ Choose ] lactose as only sugar in medium [Choose]
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- What nutrients do the following media components contain? Peptone Yeast extract Beef extract Potato infusion Agar Why should agar media be completely dissolved before they are dispensed in tubes and plates? What are the bases for pegging the temperature at 1210C for 15-30 minutes during moist heat sterilization and 1800C for two (2) hours using dry heat sterilization? 1.Can you sterilize culture media using dry heat sterilization? Why is that so? You will notice in the videos shown, the cotton plug is not used. What is role of cotton plug in media prep, sterilization and culture of microorganisms? Instead of using cotton plug, plastic screw-cap is used, can you substitute this for the former? Is it technically acceptable in microbiology?Please include sourcedo methodologies preparation of the following 6 solutions (Broth LB, LB Agar, Potato-Dextrose Agar, LB-Dextrose Broth, Saline solution 0.85%, Glycerol 80%). Include photos or schematics.All recipes are given per litre unless specified. Lambda Broth (LB) Plates Top Agar 10 g tryptone 10 g Tryptone 2.5 g NaCl 2.5 g NaCl 15 g Agar 7 g Agar Make 5 mL aliquots, then autoclave. Lambda Broth (LB) Media Suspension Media 10 g tryptone 5.0 g NaCl 2.5 g NaCl 2 g MgSO4· 7H2O 50 ml 1 M Tris pH 7.5 In LB medium…
- Can you classify the following biochemical tests based on the following criteria and explain why you did so? Test based on the presence of extracellular enzymes Test based on the presence of intracellular enzymes Test ased on carbohydrate fermentation Test based on protein catabolism 1. Catalase Test 2. Oxidase test 3. MacConkey agar (MAC) Medium Test 4. Mannitol Salt Agar (MSA) Medium Test 5. Gelatin Hydrolysis 6. Indole Production 7. Motility-Indole-Ornithine Decarboxylation Test 8. Methyl Red / Voges-Proskauer (MR/VP) Test 9. Nitrate Reduction Test 10. Simmon’s Citrate Agar Slant 11. Triple Iron Sugar Test 12. Urease TestAll recipes are given per litre unless specified. Lambda Broth (LB) Plates Top Agar 10 g tryptone 10 g Tryptone 2.5 g NaCl 2.5 g NaCl 15 g Agar 7 g Agar Make 5 mL aliquots, then autoclave. Lambda Broth (LB) Media Suspension Media 10 g tryptone 5.0 g NaCl 2.5 g NaCl 2 g MgSO4· 7H2O 50 ml 1 M Tris pH 7.5 In LB medium…Sulfur Indole Motility (SIM) Medium H2S produced, color and +/-: ______________________________ Indole present/Tryptophan hydrolysis, color and +/-: ___________________________ Motile or non-motile: _____________________________
- Time point (min) Absorbance of culture at 660nm Approximate cell concentration Approximate # cells in 1mL extract 0 0.298 1.49 x 108 cells/mL 1.49 x 108 cells 10 0.316 1.58 x 108 cells/mL 1.58 x 108 cells 20 0.374 1.87 x 108 cells/mL 1.87 x 108 cells 30 0.429 2.145 x 108 cells/mL 2.145 x 108 cells 40 0.512 2.56 x 108 cells/mL 2.56 x 108 cells 50 0.544 2.72 x 108 cells/mL 2.72 x 108 cells 60 0.607 3.035 x 108 cells/mL 3.035 x 108 cells a. Using these data, prepare a growth curve of this strain ofEscherichia coli (E. coli).b. Estimate the doubling time for this strain of E. Coli. Clearly showhow you estimated this value from the empirical data presented.Media - Possible Results Туре of Medium Original Color Any Chemicals Added to Visualize Reaction? Negative Result Positive Result Phenol Red Glucose Phenol Red Lactose Sulfur: Sulfur: SIM Indole: Indole: Motility: Motility: MR VP Citrate Slant Nitrate Broth 6.5% NaCi TSB Bile Esculin For the Fluid Thioglycollate Medium, draw the five possible outcomes in the sections below and label accordingly. (obligate aerobe, obligate anaerobe, facultative anaerobe, aerotolerant anaerobe, microaerophile) Fluid Thioglycollate Medium Novobiocin Sensitivity Test : Use the Difco Manual for the following and describe the possible outcomes: EMB: Mannitol Salt Agar:Example of a Protein Purification Scheme: Purification of the Enzyme Xanthine Dehydrogenase from a Fungus Volume Total Total Specific Percent Fraction (mL) Protein (mg) Activity Activity Recovery 1. Crude extract 2. Salt precipitate 3. Ion-exchange chromatography |4. Molecular-sieve chromatography 5. Immunoaffinity chromatography 3,800 22,800 2,460 0.108 100 165 2,800 1,190 0.425 48 65 100 720 7.2 29 40 14.5 23 1.8 275 152.108 11 Calculate the specific activity of step#4. Note that percent recovery=% Yield.
- Triple Sugar Iron slant for Staphylococcus Epidermidis , tell me the results I would get if I did this test?50 mg/50 ml kinetin stock is available You need 2.5 mg/l kn for Ms media calculate amount of kn is required? Explain the role of surface sterilising chemicals in aseptic culture initiation and function of sucrose in MS medium?Date Sheet Extraction ODD EVEN ODD EVEN GROUP Single Extraction 1 Single Extraction 2 Multiple Extraction 1 Multiple Extraction 2 Weight of tea leaves 10.002 (g) 10.123 (g) 10.013 (g) 10.007 (g) Weight of evaporating dish + caffeine 123.689 (g) 124.334 (g) 121.815 (g) 126.523 (g) Weight of empty evaporating dish 123.513 (g) 124.147 (g) 121.614 (g) 126.246 (g) Weight of caffeine Post Lab Questions Explain the principles involved in extraction. What other organic compounds can be extracted from tea bags? Draw their structures and briefly mention their biological function/s. Why do you need to add sodium carbonate in the extraction process?