Please match the type of media with its corresponding example to test your understanding of liquid, solid, and semi-solid media.

Please label the figure to demonstrate your understanding of selective and differential media. General-purpose Differential Complex medium Selective medium Simple medium medium medium

Answer the ff with not more than two sentences: Why should Erlenmeyer flasks hold not more than 60% of their capacity?  When can gelatin be used in culture media preparation?  How does cotton plug prevent contamination of the medium?  Why should caps of glassware be loosened first before sterilization?

Explain how to prepare 250 ml liquid and 500 ml solid form separately from LB (Luria Bertani) medium whose components and concentrations are given below. Yeast extract 5 g/L Peptone 10 g/L NaCl 10 g/L Agar 1.5 g/L

Describe the main purposes of media, and compare the three categories based on physical state, chemical composition, and usage.

Identify the qualities of enriched, selective, and differential media;use examples to explain their content and purposes.

EMB agar inhibits the growth of Gram-positive bacteria while encouraging the growth of Gram-negative bacteria. It also can determine if some bacteria use lactose. It is particularly effective at determining if E. coli is present in a specimen. Define what type(s) of media EMB agar is, using as many terms in the answer list as are applicable.

Name at least one reason that we might want to use a Winogradsky column rather than a laboratory medium such as nutrient agar or beef heart agar. Describe why metabolism in the presence of oxygen might cause problems for microorganisms.

In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down. For "autoclaving" For Dry heat oven Can be sterilized only sterilization with either Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-ml graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-ml beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropper

You are asked to determine the settleability of biomass in a suspended growth activated sludge sample that was brought to the laboratory. Describe the protocol you follow to achieve the objective. (If you prefer it, you can use your own numerical example to illustrate the process). Use drawings if you like to illustrate your point(s).