1 H2176old cultureSmear tooBOTERCLosklike?+b. Failure to apply the decolorizer.+nec. Failure to apply the safranin.Determine whether bacteria are motle or non-motle by looking at the growth in semi-solid mediumWhat is the concentration of agar in (a) typical solid medium (b) semi sold motility medium?Arawer Q1 on Page 357.7. Orygen Requirement:+d. Reversal of crystal violet and safranin stains.A questions 1.2 and 3 o Pg 83-84Refer to the power pointQuestions1Predict the effect of the following "mistakes" made when performing a Gram stain. Your answer needs to addressthe predicted effect on both Gram-positive and Gram-negative cells. Consider each mistake independently.a. Failure to add the iodine.414gait to fer.2 Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram-positive andGram-negative cells. This being the case, explain how they stain different cell types in the Gram stain.why?a cell wall.patem in thioglycolate3If you saw large, eukaryotic cells in the preparation made from your gumline, they were most likely your ownepithelial cells. Are you Gram-positive or Gram-negative? What is it about your cell structure that would allowyou to predict this result?Pink, wedo not haveWhat isThe do trols have pink cells (one more than the other). What might you suggest she try and why?wrong4 One of your lab pOne of your lab partners has followed the recommended procdure of running Gram-positive and Gram-negativecontrol organisms on her Gram stain of an unknown species. Her choices of controls were Escherichia coli andBacillus subtilis. She tries several times and each time concludes she is decolorizing too long because both con-cell wallProtectsand otheris not.Explain.MICROBIOLOGY: LABORATORY THEORY AND APPLICATION, BRIEF1

In order to understand the mistakes made in Gram staining procedure we need to understand the whole…

Answered: Questions 1 Predict the effect of the…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Answer the following questions briefly and concisely 1.How do bacteria in a chemostat and those in a batch culture vary from one another? 2. What happens in a chemostat if the dilution rate is higher than the organism's maximum specific growth rate? 3.Does a chemostat require the use of pure cultures? 4. Why would a complicated culture media for Leuconostoc mesenteroides be simpler to make than one with a fixed chemical composition?
use these OD numbers to plot growth curve for E. coli K12- and estimate generation time for this culture. Table 1. Absorbance (O.D.600) measured for growing culture of E. coli K12 (time course) Incubation time O.D.600 Incubation time O.D.600 O min 3 hrs 3 hrs 30 min 4 hrs 4 hrs 30 min 5 hrs 5 hrs 30 min 0.11 1.75 30 min 0.20 1.94 1 hr 1 hr 30 min 2 hrs 2 hrs 30 min 0.27 2.24 0.51 2.48 2.31 2.19 0.65 1.30
a.Describe what makes thioglycollate medium suitable for culturing anaerobes. What would the growth patterns of Clostridium sporogenes and Micrococcus luteus be in this medium? b. In the Kligler test, why do we inoculate the surface of the agar slope and then stab into the butt of the slope? What does a pink coloured colony indicate when using MAC (MacConkey Agar)?
procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (2) agar slant culture to agar slant
A 1.5-year-old child developed vomiting, diarrhea, and fever. Stool sample were inoculated into the Endo media. After 18 hours on the surface of the medium grew medium-sized, round, slightly convex red colonies with a metallic luster. The doctor suspected Escherichia coli. 1. Name the composition of the Endo agar media. 2. Describe the properties of bacterial colonies on the Endo media. 3. What purpose differential diagnostic Endo media used for?
4. Which ranges of microbial colony count is ideal standard for a good 1, plate in Pour Plate technique? Check all that apply. * O TNTC (too numerous to count) O 25-250 colonies O 10-1000 colonies O 30-300 colonies 5. How many colony-forming units (CFUS) of bacteria are there in a gram of soil if 120 colonies grow in a nutrient agar (NA) plate with 1 ml inoculum from 1:10,000 dilution? * 120 O 1,200 120.000 1,200,000 6. How much volume of microbial inoculum will be dispensed into a sterile nutrient agar plate in Spread Plate technique of bacterial isolation? 0.1 ml 1 ml 10 ml O 100 ml 7. Which materials are to be prepared when using Spread Plate technique for bacterial isolation from water sample of oligotrophic Lake Lanao? Check all that apply. * O lake water sample O sterile nutrient agar plate O sterilizable glass rod O flame source
CITRATE TESTThis test determines the ability of bacteria to use citrate as source of carbon andinorganic ammonium salt as a source of nitrogen. The following are the stepwise procedurein conducting citrate test:1. Infuse the Simmons citrate agar on the slant by touching the tip of sterilized needleto a colony.2. Incubate at 37 degree Celsius for 24 to 48 hours.A positive result is indicated by the pH indicator, bromothymol blue which turns the growthgreen to blue. Task: In a tabulated form, determine if the Pseudomonas aeruginosa, are positive or negative withcitrate test. Interpret your answer and include the reaction of organism on this test.
CITRATE TESTThis test determines the ability of bacteria to use citrate as source of carbon andinorganic ammonium salt as a source of nitrogen. The following are the stepwise procedurein conducting citrate test:1. Infuse the Simmons citrate agar on the slant by touching the tip of sterilized needleto a colony.2. Incubate at 37 degree Celsius for 24 to 48 hours.A positive result is indicated by the pH indicator, bromothymol blue which turns the growthgreen to blue. Task: Answer the following In a tabulated form, determine if the Salmonella typhi, are positive or negative withcitrate test. Interpret your answer and Include the reaction of organism on this test.
CITRATE TESTThis test determines the ability of bacteria to use citrate as source of carbon andinorganic ammonium salt as a source of nitrogen. The following are the stepwise procedurein conducting citrate test:1. Infuse the Simmons citrate agar on the slant by touching the tip of sterilized needleto a colony.2. Incubate at 37 degree Celsius for 24 to 48 hours.A positive result is indicated by the pH indicator, bromothymol blue which turns the growthgreen to blue. Task: In a tabulated form, determine if Salmonella typhi the are positive or negative withcitrate test. Interpret your answer and include the reaction of organism on this test.
Match the description to the correct medium This medium is enrichment for fastidious [Choose ] bacterial pathogens and differential based on hemolysis [Choose ] This medium is selective based on Tryptic Soy Agar, TSA salt/sodium chloride tolerance at 7.5 % Sabouraud Dextrose Agar, "Sab" agar weight/volume and differential based on mannitol fermentation Mannitol Salt Agar, "MSA" MacConkey Agar, "Mac" agar This medium is selective based on the ability to grow in bile salts and crystal violet and is EMB Eosin Methylene Blue Agar differetial based on lactose fermentation Blood Agar, "BA" this medium is all purpose and can grow a [Choose ] wide range of bacteria and fungi
If 5% w/v concentration of a bactericide of dilution coefficient of 3 takes 25 minutes to effect killing, what will be the time of kill when it is diluted 8 times A sanitizer with dilution coefficient of 0.5 takes 3 minutes to effect killing. If it takes 18 minutes to kill when it is diluted 5times, what is the initial concentration? An unknown concentration of ‘LD’ disinfectant killed 106 viable cells of Sporotrichum schenkii within a certain time. When the concentration was increased 2-fold the extinction time decreased a 103-fold. Determine the concentration exponent of this disinfectant
1. What is the purpose of adding mineral oil to an O/F Media tube and why do we only put mineral oil in only one? 2. Which sugars are typically tested in the phenol red broth? Can the phenol red test be also used to determine if a certain bacterium can metabolize various carbohydrates? 3. Explain how conducting the MR/VP test too soon in a culture's growth cycle can lead to false negatives? 4. Generally, the color change we see in these tests is a result of what change in the media? Explain. 5. How does the production of urease acid aid Helicabactor. pylori ability to live in the stomach?

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Questions 1 Predict the effect of the following "mistakes" made when performing a Gram stain. Your answer needs to address the predicted effect on both Gram-positive and Gram-negative cells. Consider each mistake independently. a. Failure to add the iodine. b. Failure to apply the decolorizer.