d)Halfway through the electrophoresis of your gel you realized that rather than using a buffer to make up your gel, you actually used distilled water. Do you think this mistake would alter the outcome of your gel electrophoresis? Explain. e) If you added your DNA to a well in a gel without first mixing it with gel loading solution, would this impact the final results? Explain. f) Only after the run you realize that you forgot to add ethidium bromide to the gel. What will happen to your sample? What could you do to the gel after the run?
A health provider contacts you regarding a specific patient. They ask you to sequence this patient’s DNA and sends you her blood sample. You split the patient’s blood sample into three equal parts. With each part, you perform three different protocols of DNA extraction and purification. You analyze the resulting DNA using the nanodrop.
| Protocol | A260/280 | ng/ul DNA | Total volume (ul) |
| 1 | 2.02 | 50 | 60 |
| 2 | 1.45 | 156 | 200 |
| 3 | 0.30 | 300 | 120 |
d)Halfway through the electrophoresis of your gel you realized that rather than using a buffer to make up your gel, you actually used distilled water. Do you think this mistake would alter the outcome of your gel electrophoresis? Explain.
e) If you added your DNA to a well in a gel without first mixing it with gel loading solution, would this impact the final results? Explain.
f) Only after the run you realize that you forgot to add ethidium bromide to the gel. What will happen to your sample? What could you do to the gel after the run?
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