When y

What type of matrix was used to separate DNA in gel electrophoresis? Agarose 1X TBE Glycerol Polyacrylamide Ο 1X TAE

How many microliters of pGLO plasmid will you need for a PCR reaction is you need 200 nanograms and the plasmid concentration is 0.05 micrograms/microliter?

After plasmid isolation, you take 0.5 uL of the sample and mix it with 1 uL of TE buffer. The absorbance of this sample at 260 nm is 0.8 and the average extinction coefficient of double-stranded DNA at 260 nm is 0.020 (μg/ml)−1 cm−1. What is the concentration of the undiluted sample?     120 μg/mL     80 μg/mL     40 μg/mL     12 μg/mL

You start running your gel and after a few minutes notice that the colored bands from the loading dye are moving in the wrong direction. This is happening because you accidentally: O Forgot to add loading dye to your DNA samples O Forgot to add ethidium bromide to your DNA samples O Attached the negative electrode to the gel box on the end where samples were loaded Attached the positive electrode to the gel box on the end where samples were loaded

You digest 4 uL of plasmid DNA that is 50 ng/uL concetration in a total volume of 20 uL. You run 10 uL of the digest on teh gel. You then do a DNA purification protocol with a Zippy prep on the remaining digested DNA. You elute the DNA in a 25 uL. A 2 uL ssample has the concentration of 2 ng/uL. What is the DNA yield?

A health provider contacts you regarding a specific patient. They ask you to sequence this patient’s DNA and sends you her blood sample. You split the patient’s blood sample into three equal parts. With each part, you perform three different protocols of DNA extraction and purification. You analyze the resulting DNA using the nanodrop. Protocol A260/280 ng/ul DNA Total volume (ul) 1 2.02 50 60 2 1.45 156 200 3 0.30 300 120 d)Halfway through the electrophoresis of your gel you realized that rather than using a buffer to make up your gel, you actually used distilled water. Do you think this mistake would alter the outcome of your gel electrophoresis? Explain. e) If you added your DNA to a well in a gel without first mixing it with gel loading solution, would this impact the final results? Explain. f) Only after the run you realize that you forgot to add ethidium bromide to the gel. What will happen to your sample? What could you do to the gel after the run?

Which of the following gels is the preferred choice for separation of DNA fragments? Agarose Polyacrylamide SDS PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis) Agar

DNA is visualized during agarose gel electrophoresis by ______________ . the fact that DNA fluoresces when illuminated with UV light the binding of a fluorescent dye that is easily detectable using radioactive antibodies that specifically bind to DNA the fact that DNA is blue and can be seen when millions of copies are present in a band

A student is trying to add 9.0 pmol of primer mix to a 20.0 µL PCR.  The primer mix is at a concentration of 50.0 µM, and the student determines that a serial dilution is required because directly adding the primer mix would require a volume less than 1.00 µL.  The student takes 1.40 µL of the primer mix stock and mixes it with 25.0 µL of ultrapure H2O.  Calculate the volume (in µL) of the intermediate solution required to add the correct amount of primer mix to the PCR.