Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN: 9781133893943
Author: ESTRIDGE
Publisher: Cengage
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- You are a forensic scientist in charge of DNA samples in a case where a man was murdered. You conduct gel electrophoresis on the samples to determine the DNA profile of the samples. The rows of bands have been marked to make it easier to reference the bands. The sample marked 'evidence' came from under the victim's fingernails, likely indicating a struggle. There are two suspects in the case, both of whom are brothers, they are marked suspect 1 and suspect 2, for brother 1 and brother 2, respectfully. Does the evidence collected match the DNA profiles of either brother? If yes, which one? If no, then would it be likely that the actual criminal is a family member of the brothers? If yes, what could the relation be (mom, dad, cousin, aunt, or uncle)? Note: there is a sister in their family, their parents are both still alive, and they have a robust extended family. The brothers are not twins.arrow_forwardSTR analysis was performed on blood from a crime scene as well as from three suspects. The crime scene DNA is marked Evidence. Which suspect’s DNA matches the evidence?arrow_forwardThe following gel shows the results of a crime scene investigation. Lane 1 shows a DNA sample that was obtained from the scene of a crime (there was evidence of the criminal cutting himself, so it's the criminal's blood sample that produced the DNA bands in lane 1). Lanes 2-7 shows a DNA sample from six potential suspects held in connection with the crime. Arrange the bands labeled A, B, C, D from largest to smallest. 1 2 3 4 5 6 7 OC, A, D, B A, B, C, D OB, D, A, C O D, A, C, Barrow_forward
- THe DNA profiles of five people potentially involved in a robbery are shown in DNA Profile #2. The profile on the left side was obtained from DNA belongíng the robber. The four other profiles were obtained from the suspects. Which of the potential suspects is most likely the robber?arrow_forwardHelparrow_forwardA. Use your pencil to find out if the DNA of the suspect agrees with the DNA from the evidence in Figure 5. Circle your answer. YES NO B. Use your pencil to find out if the DNA of the suspect matches the DNA of the evidence in Figure 6. Circle your answer. YES NO E Figure 5 s " E Figure 6 2. In Figures 7 and 8 you will see if there is HORIZONTAL agreement or matching between suspect and evidence. Use your pencil to make these matches. Underline your choice. A. Suspect DNA and Evidence DNA (match, don't match) in Figure 7. A. Suspect DNA and Evidence DNA (match, don't match) in Figure 8. S E Figure 7 1 #11111 11 ||||||| 1 E Figure 8 1271WS4Parrow_forward
- DNA Sample : Blood Stain collected from handle of knife blood Bob Sue John Lisa stain 1. What do you notice about the black lines? 2. How do we read the gel electrophoresis? 3. Which sample are we comparing Bob, Sue, John, and Lisa's DNA to? 4. How do they compare?arrow_forwardWhat does a guy need to do (e.g. how different does his DNA profile need to be?) to be excluded as a possible contributor to a DNA profile from an evidence sample?arrow_forwardThe scientists were able to collect DNA from the individual’s body. True Falsearrow_forward
- Questions: Answer questions 1 to 3 about this gel electrophoresis. 1. Which color represents the smallest DNA fragment? (explain) 2. If “A" is the crime scene and B to E are suspects. Do you have any match? (Why or Why not) 3. Is there a possibility that any of the suspect related to the person who committed the crime? Explain.arrow_forwardCan you please check my answer and make sure it is correct. Question: How can DNA evidence be used to convict or exonerate a defendant? Why is DNA evidence so powerful? Answer: DNA evidence can be used to perform DNA profiling to determine the genotype of the specific DNA sample. With just a small amount of DNA, PCR can produce billions of copies of that specific segment. The segments that are used are from non-coding regions that contain STR’s or short tandem repeats. These very short DNA sequences are repeated and are specific to individuals because we inherit them from our mother and father. Gel electrophoresis separates the PCR products based on their size and each band is compared to the allele ladder. This process helps to identify the alleles present in the original samples. DNA profiling is performed at many loci to be able to tell the genetic difference between different individuals with a lot of certainty. The DNA from the different suspects is compared to the allele…arrow_forwardGel Electrophoresis is used often in forensics. Look at the following gel to the left. From the evidence DNA, which individual matches the DNA evidence left at the crime scene?arrow_forward
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