True or false: During DNA isolation, ice cold 95% ethanol is used to digest the DNA into fragments that can be collected for running in an electrophoresis gel. O True O False

Q: In this activity you will extract DNA from living material. Before you begin, here are some helpful…

A: DNA (deoxyribonucleic acid) extraction is the process in which DNA of the given living organism or…

Q: What is the probability of finding a 6 base pair palindrome in a sample of DNA? Answers A-E A 1/4 B…

A: Introduction  A palindrome sequence is a sequence of nucleotides (DNA) or amino acids (proteins)…

Q: An AGE run was set at 100V for 30 min, where 3 ul of the ladder (100 ng/ul) was loaded into the gel,…

A: if 3ul of DNA ladder is added on the gel AGE which has a concentration of 100ng/ ul. Then it means :…

Q: Reagents Sterile ddH₂0 10X PCR Buffer (E) mM MgCl₂ 10mM dNTP (1) mM Forward Primer 50 mM Reverse…

A: Polymerase chain reaction (abbreviated PCR) is a laboratory technique for rapidly producing…

Q: DNa Mapping using Restriction enzymes lab: We will be aliquoting and delivering 5 μl of enzyme to…

A: A restriction enzyme, also known as restriction endonuclease is an enzyme that cleaves DNA into…

Q: A DNA fragment with 450 bp will be closer to the top (negative pole) of an electrophoresis gel than…

A: Friedrich Meischer discovered nucleic acids from the nuclei of pus cells and coined the term…

Q: Which of the following is the sequencing gold standard? Oion semiconductor sequencing O sequencing…

A: DNA sequencing is the process of determining the precise order of nucleotides (A, T, C, and G) in a…

Q: When gel electrophoresis is undertaken, one well always contains DNA-marker molecules. The purpose…

A: The objective of the question is to understand the purpose of DNA-marker molecules in a gel…

Q: TRUE OR FALSE: Mupirocin inhibits bacterial cell wall synthesis. Mupirocin is used as a negative…

A: we have to answer wether the given statement is true or false . Due to question answering policies…

Q: During gel electrophoresis, DNA fragments are separated such that the longest DNA samplas migrate…

A: It is false   The true statement is- during gel electrophoresis DNA fragment are separated such that…

Q: When choosing a DNA extraction protocol, which of the following should be first taken into account?…

A: DNA extraction is a process of isolating DNA from biological samples, such as cells, tissues, or…

Q: You are studying the DNA binding protein CLAMP and you want to determine its binding affinity for…

A: Introduction DNA acts as genetic material in our body. It is a double stranded molecule. mRNA is…

Q: Restriction enzymes bind to specific sequences of DNA to seal them together. True False

A: Restriction enzymes bind to speffic sequence of DNA seal them together. This is TRUE. Restriction…

Q: a.What is the importance of centrifugation in step 3?  b.

A: Introduction: For the development of diagnostics and medications as well as for research into the…

Q: What is the purpose of adding dishwashing liquid into the banana mixture? What reagent is used in…

A: DNA extraction is the method of isolation of genetic material ( DNA ) from plants , animals or any…

Q: PCR technique is based on DNA transcription.  True  False

A: Introduction : PCR (polymerase chain reaction), the reaction mixture includes the DNA extract…

Q: When the SEBI gene is inserted with a defective leading to phenotype. bacterial DNA, wrinkle…

A: Phenotype refers to the observable physical or biochemical characteristics of an organism resulting…

Q: 1. From one colony of E.coli, you have purified the DNA of a recombinant plasmid. You have…

A: The DNA is measured at absorbance 260 and 280nm, and the ratio of two readings will tells the purity…

Q: Each PCR cycle has three steps: DNA sample denaturation, primer annealing, and elongation/extension…

A: Polymerase chain reaction (PCR) is a simple technique to amplify a DNA template to produce specific…

Q: Aside from the sample and assuming proper AGE protocol, which of the following is most needed to…

A: Agarose gel  electrophoresis is an important technique used in molecular biology research. In case…

Q: The movement of sucrose molecules across the plasma membrane takes place through Select one: a.…

A: A transport protein (variously observed as a transmembrane pump, transporter, escort macromolecule,…

Q: Assume the samples in this diagram of gel electrophoresis are DNA molecules. Why do the samples…

A: Gel electrophoresis is used to separate molecules of different sizes. It uses charged molecules that…

Q: What is the size of the DNA sample based on the gel profile below? Make sure to indicate the units.…

A: Electrophoresis is the technique to separate biomolecules depending on their…

Q: Loading dye stains the DNA molecules and thus help to visualize it under UV light apparatus. O True…

A: DNA Loading dye is used to monitor the migration of DNA into the gel during electrophoresis.…

Q: (Final) for optimization Volume (uL) for 1x Volume (uL) for 1(7)x Components Initial Sterile 8…

A: Polymerase Chain Reaction ( PCR ) -- Polymerase Chain Reaction or PCR is a technique to make many…

Q: Compared to a 3% agarose gel, how far should the same size DNA travel in a 1% agarose gel? not as…

A: The DNA has a uniform mass proportion, DNA particles are isolated by size inside an agarose gel in…

Q: Which of the following are true about ddNTPs. Select all that apply. They cause DNA synthesis to…

A: Dideoxynucleotides are nucleotides that lack a 3'-OH group, which helps in the termination of DNA…

Q: sample containing at least one molecule of DNA serves as the starting material for PCR.  True…

A: PCR or polymerase chain reaction is routinely used in molecular biology laboratories that ultimately…

Q: In Polymerase Chain Reaction (PCR). Primers are short segments of DNA that. Obalance the pH of a…

A: Polymerase Chain Reaction or PCR is a molecular technique allowing the synthesis of multiple copies…

Q: The solid matrix that is usually used to attach the target DNA in southern blotting technique is…

A: Blotting is utilized in atomic science for the distinguishing proof of proteins and nucleic acids…

Q: write true if the statement if correct and change the " " word/phrase to make it correct   the DNA…

A: Answer :- The above statement is correct

Q: Evaluate the gel provided below. Use the notes to help you read the gel: DNA is loaded in the wells…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Answer choices : 0.3% gel OR 1.0% gel Explanation Choices : Agarose makes smaller pore sizes which…

A: Electrophoresis is an analytical technique that separates the biomolecule on the gel matrix based on…

Q: Recombinant DNA techniques that require gel electrophoresis

A: Following are the applications of gel electrophoresis in DNA recombinant technology.

Q: Samples came to the laboratory for analysis. You are asked to identify the differences between these…

A: Spectrophotometer is used to determine the concentration of any unknown sample. DNA is a…

Q: DNA Extraction and Characterization from Shrimp Describe the resulting gel, assess the purity of…

A: Here, given is a picture of sample gel. The distance of migration of bands in the ladder is given in…

Q: 1 2 3 4 M 1000 b 900 bp 800 bp 700 bp 600 bp 500 bp 400 bp 300 bp 200 bp

A: The diagram is showing the separation of DNA fragments using gel electrophoresis.  In-gel…

Q: Look over the protocol for the PTC lab. If the tube containing the DNA and HaeIII were NOT incubated…

A: HaeIII is a type II restriction endonuclease enzyme that recognizes and cleaves DNA at a specific…

Q: Gel electrophoresis is a laboratory technique that separates molecules by size using an electric…

A: The laboratory method used to separate the mixtures of DNA, RNA or proteins according to their…

Q: The sample furthest to the left contains the DNA size standards. What is the purpose of this sample?…

A: Gel electrophoresis is an analytical technique in which DNA fragments in a sample are separated…

Q: Which of the following is not true about microarrays? Group of answer choices A microarray is a…

A: Introduction :- DNA microarray ( also known as gene chip , DNA chip, bioarray or gene array ) is an…

Q: You start running your gel and after a few minutes notice that the colored bands from the loading…

A: Agarose gel electrophoresis is a form of electrophoresis which is used for isolating DNA fragments…