True or false: During DNA isolation, ice cold 95% ethanol is used to digest the DNA into fragments that can be collected for running in an electrophoresis gel. O True O False

DNA Extraction Using household materials Wash the fruit. Place the fruit (e.g. banana) in a zip lock plastic bag and crush it with your fist. Add the one half teaspoon of salt and one tablespoon of dishwashing liquid to the mashed fruit mixture in the bag, zip it up and squeeze it in your hands for 1 minute. Place the strainer over the small glass. Pour the mixture into the strainer. Filter the mixture into the glass. Slowly pour ice cold rubbing alcohol into the glass, until it is about half full. Let it stand until the alcohol will form a separate layer on top of the fruit filtrate. Keep the glass still at eye level; do not shake it. Watch what happens. DNA will begin to appear where t 9. Carefully Scoop out the DNA with the straightened end of the paper clip. Touch the DNA. he alcohol and filtrate layer meet A. In step 4, isopropyl alcohol is used to precipitate out the DNA because it is not soluble. What does this mean?  B. What role did the alcohol, detergent and salt play?…

The table shows the presence/absence and size of DNA bands of known samples using gel electrophoresis. Explain conclusions you can draw from the data in the table.

. Below is a plasmid map of pBR328, describe its screening procedure. Ncol Mscl EcoRI BspEl Pvull Cm Pvul pBR328 4907 bps Amp Clal Hindill Pstl EcoRV BamHI Tet ColE1 ori Sall