Describe the experiments done by Hershey and Chase That Verified That DNA was the genetic material. Make sure you also include their conclusions.
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3. Describe the experiments done by Hershey and Chase That Verified That DNA was the genetic material. Make sure you also include their conclusions.
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- 2. Who are the scientists that proved DNA is the transferrable genetic material and Discuss how they proved based on their experiment?4. Explain why Maurice Wilkins and Rosalin Franklin needed to use X-rays (instead of visible light) when "taking pictures" of crystalized DNA.1. List down at least 5 things that doesn’t have DNA and why? 2. In doing a Laboratory experiment do you think there are tools and components that are more important than the other? Why? Answer question 1 and 2.
- 3. DNA fragmentation is the separation or breaking of DNA strands into pieces. Why is it important to identify different DNA fragments in DNA profiling? 4. You and your team have taken a magnifying glass to look at the evidence so that you can compare it to the DNA of the 4 suspects. Which suspect do you think committed the crime based on the results from the gel electrophoresis? Use evidence from the diagram and what you know about the process to justify your response. 5. How is the evidence from earlier years vs. evidence used now changed? Evidence DNA Lepadsns Suspect #2 Victim Officer5. Watch the video: https://www.youtube.com/watch?v=ml0Fo9kaWqo&t=38s. Based on the information in this video and material covered in class answer the following questions: A. In a Sanger sequencing-based PCR reaction, how many primers are added? B. In a Sanger sequencing-based PCR reaction, which molecules are more common: DNTPS or fluorescent terminators? C. What is a typical length of sequence that can be acquired using traditional (Sanger) sequencing? D. The results from sequencing one stretch of DNA is called a "read". How many reads can be obtained from a run on a 384-well plate Sanger sequencing instrument vs. a NovaSeg instrument? E. How many gjgabases of sequences can be obtained on the Sanger vs. NovaSea instruments? Regarding F. and G., the adaptor sequences have two parts: primer binding sites and capture sequences. F. Once the (denatured) DNA has been loaded into the flow cell, which adaptor sequence part is hybridized to other single-stranded DNA first? G. How many copies…2. Can you develop a molecular biology assay to help forensic scientist to identify the criminal from the victim? Try to explain the concept behind the assay.
- 3. Match the following biotech tools with their applications. Some of those tools may have more than one applications. • A. Gene cloning and Recombinant DNA • B. Restriction enzyme • C. Radioactive nucleic acid probe • D. Reverse transcriptase • E. Ti plasmid. • F. Gene therapy • G. PCR • H. Gel electrophoresis • I. single nucleotide polymorphism (SNP) and Restriction fragment length polymorphism (RFLP) • J. Genomic 1. Crime scenes and paternity 2. Making copies of DNA sequence 3. GMO 4. introduce new genes into plant cells 5. alteration of an individual's DNA to treat disease. 6. Cutting DNA at target sequence 7. Detect the presence of specific gene 8. produce a DNA strand from MRNA. 9. separate DNA molecules based on size 10. methods to get the DNA fingerprint of different individual6. Describe the process of DNA sequence of interest detection, using these terms appropriately, below. YOU MUST UNDERLINE EACH TERM AS YOU USE IT IN YOUR DESCRIPTION. a. Double-stranded DNA b. Probe c. Radioactive tag d. Gel electrophoresis e. Filter paper f. Denaturation g. Hybridization h. Single-stranded DNA i. Known sequence j. Complementary9) The diagram to the right represents one technique used in biotechnology. The organic compound used to cut the bacterial DNA so that the human DNA could be inserted a(n)
- 1. Electrophoresis is a method of (highlight the correct answer) (1) separating DNA fragments (2) changing the genetic code of an organism (3) indicating the presence of starch (4) separating colored compounds on a strip of paper1. What is the purpose of adding of (a) warm salt water, (b) detergent, and (c) alcohol on the experiment of the extraction of DNA from a banana?5. What would have happened if the gel were placed backwards in the electrophoresis chamber with the wells containing the DNA near the positive end of the gel? smiT 3 vea deJ ba ovitsgo- in Suspect #1's sample? ellow- 6b. How many DNA fragments were in Suspect #2°s sample? 6a. How many NA fragments 7. Suspect #1 and Suspect #2 have different numbers of fragments. Explain why DNA samples from different people can have different numbers of fragments. bn ovitieo+ 8. Explain the difference between tracking dye and DNA stain.