7. How could genetic engineering be of use to humans? Describe at least 2 different uses.

DNA Restriction, Electrophoresis 1. What are restriction enzymes? 2. How do restriction enzymes function? 3. Why are restriction enzymes important in biochemistry labs? 4. What is DNA Gel Electrophoresis? 5. How does DNA Gel Electrophoresis function? 6. Why is DNA Gel Electrophoresis important in biochemistry labs?

1. Describe how the plasmid is cut open by genetic engineers?2. How is the insulin gene removed from the strand of human DNA?3. Explain why the sticky gene fits into the opening of the plasmid.4. How does genetic engineering help those who are insulin dependent diabetics? Explain thoroughly

1.  What are the reaction components, and what equipment do you need for PCR?2. What can be possible source of DNA for PCR?3. What are some uses for PCR?

How would you go about cloning this amplified DNA into pL4440？Using your knowledge of cloning describe 5 important aspects of the method in details

56. In gel electrophoresis, DNA is made visible with 5o. Use the following information to answer the next question. autoradiographic blue. a. Na - K pump situated in the neural membrane b. RFLP dye. C. agarose. d. polyacrylamide gel. Outside Inside ----K* diffusion e. ethidium bromide. Na carier 57. Gene therapy alters a patient's genome by Na* diffusion - adding bacteria to the genome. excising nucleotides. adding a corrected gene. engineering transgenic tissue. a. b. c. Which of the following correctly describes the ion flow into or out of a neural fibre during repolarization? d. e. bioremediation. K* flow out K* flow in a. b. 58. Use the following information to answer the next question. Na' flow out Na' flow in c. d. Neuron none of these e. Use the diagram given below to answer the next question. 61. Human Brain Myelinated neurons conduct impulses faster than unmyelinated neurons because in myelinated fibers, sodium gates open only a dendrite labeled 1 dendrite labeled 4 a. 2- b.…

7.  Electroporation is a physical method of transferring genetic material into the cells.   True   False 8.  You have completed an in vitro mutagenesis experiment to create an G to T mutation in the coding sequence of the heat shock protein gene.  This gene is cloned in a plasmid and you want to confirm that the mutation was correctly introduced.  Which sequencing technology be most appropriate to use practically and economically:     Microarray   Sanger sequencing   RNA sequencing   Exon sequencing