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- 4. Explain how and why a researcher might use a DNA probe.3. Match the following biotech tools with their applications. Some of those tools may have more than one applications. • A. Gene cloning and Recombinant DNA • B. Restriction enzyme • C. Radioactive nucleic acid probe • D. Reverse transcriptase • E. Ti plasmid. • F. Gene therapy • G. PCR • H. Gel electrophoresis • I. single nucleotide polymorphism (SNP) and Restriction fragment length polymorphism (RFLP) • J. Genomic 1. Crime scenes and paternity 2. Making copies of DNA sequence 3. GMO 4. introduce new genes into plant cells 5. alteration of an individual's DNA to treat disease. 6. Cutting DNA at target sequence 7. Detect the presence of specific gene 8. produce a DNA strand from MRNA. 9. separate DNA molecules based on size 10. methods to get the DNA fingerprint of different individual1. Three basic steps of Genetic engineering * 2. Recombinant DNA technology has provided a broad range of tools to help physicians in the diagnosis of diseases. Probes are now used for identification of infectious agents such as
- Gene therapy can be used to introduce foreign DNA into cells. for molecular cloning by PCR of tissues to cure inheritable disease all of the above4. When and how were fingerprints first used in the United States? 5. Describe the difference between classic fingerprinting and DNA fingerprinting.4. Explain the mechanisms of SYBR green and TaqMan in real-time PCR. 5. Explain what Ct mean and what it tells us about our samples in real-time PCR. 6. Explain why a positive test for COVID19 would appear sooner than a negative result when using real- time PCR to test if someone is infected.
- 1. What is the purpose of recombinant DNA technology? 2. Why do scientists need the insulin gene in order to create medicine for humans? 3. Describe the process of recombinant DNA. Do you think this process is ethical why or why not?1.Explain the purpose and overall technique for the following biotechnology applications: a. Isolation of Nucleic Acids b. Gel electrophoresis c. Polymerase Chain Reaction (PCR)2. What components do you need to perform PCR? 3. What is in the master mix and why do you need each component? 4. Why do you need to perform PCR on DNA evidence from a crime scene? 5. What steps make up a PCR cycle, and what happens at each step? 176 Introduction to PCR-Based Forensics
- 1. How may recombinant DNA molecules be introduced into human cells? a. by splicing the needed genes into a mammalian chromosome using restriction enzymes. b. by adding plasmids to the mammalian cells. c. by using engineered viruses as vectors. d. by using a gene gun. 2. If someone is accused of a crime, investigators can collect his or her DNA to compare the DNA of the cells found at the crime scene. To collect human DNA, investigators often swab the inside of person’s cheek. Just a few human cheek cells contain enough material to perform PCR. In a cell, the nucleus and mitochondria contain DNA that is the starting material for PCR. Identify the 4 components needed to start a PCR reaction (equipment not included)1. What is the difference between an antigen test and a PCR test? a. What are the benefits of using each and the drawbacks to each?18. CRISPR can change DNA in animals. What did scientists look at in the nature to help them create CRISPR? Question 18 options: Bacteria fighting off viruses Sheep cloning antibiotic resistance cell survival 19. Identify the disease: •It affects mainly males. •Affected males are usually born to unaffected parents; the mother is normally an asymptomatic carrier but may have affected male relatives. •Females may be affected if the father is affected and the mother is a carrier. Question 19 options: X-linked recessive X-linked dominant Y-linked Autosomal recessive
