Q: What are the interesting characteristics of DNA quantitation? What are the positive and negative…
A: DNA quantification is done to know it's concentration and purity of the sample. This can be done…
Q: Scientists have reportedly isolated short fragments of DNA from fossilized dinosaur bones hundreds…
A: The contamination of deoxyribonucleic acid (DNA) defined as the inadvertent addition of an…
Q: In the given below option which of the following are considered as the main tools for DNA…
A: DNA technology alters the phenotype of the organism through a genetically altered vector. It…
Q: In this activity you will extract DNA from living material. Before you begin, here are some helpful…
A: DNA (deoxyribonucleic acid) extraction is the process in which DNA of the given living organism or…
Q: Which of the following is NOT a characteristic of PCR primers? Short synthetic oligonucleotide…
A: Polymerase chain reaction is a procedure in which a strand of DNA is amplified by using short RNA…
Q: How many fragments would be produced if the DNA is cut by that enzyme? Number each fragment…
A: * Restriction enzymes recognizes specific palindrome sequence and cut DNA only at that specific…
Q: primer
A: PCR- it stands for the polymerase chain reaction. it is used to make many copies of a specific…
Q: What does the alcohol do in this experiment? Why does the DNA rise to the top after adding alcohol?…
A: Extraction of DNA from fruits.
Q: Today, it is easy to make transgenic plants and animals. What are some important safety and ethical…
A: Recombinant DNA technology Insertion of desired foreign DNA in an organism.
Q: Describe and contrast the common steps of DNA replication in vivo and the PCR reaction in vitro?
A:
Q: If you were to set up a PCR reaction (in vitro DNA synthesis) with a DNA template, primers,DNA…
A: PCR is an in vitro technique for the DNA synthesis. It is used to generate the multiple copies of…
Q: The number of DNA molecules produced after 30 cycles of PCR, starting with a single molecule, is…
A: Polymerase Chain Reaction (PCR) is a gene or DNA amplifying instrument widely used in biotechnology.…
Q: The original DNA template starts with T following the primer sequence if the smallest fragment…
A: Dideoxy nucleotides are similar to regular, or deoxy, nucleotides, but they lack a 3’ OH group.…
Q: Choose the basic chemical materials that are required to perform PCR. A. Two oligonucleotide…
A: Note :- As per bartleby guidelines experts are supposed to only answer one question at a time.…
Q: What animals are usually used by scientists when performing Recombinant DNA? Why do
A: Recombinant DNA Technology -- The technology of recombinant DNA was developed in 1973 by Boyer and…
Q: Which of the folllowing is the term used for ultra compact form of DNA coiling? a. supercoiling…
A: Compacting DNA is important for packaging it within the cells. As the length of a DNA molecule can…
Q: By seeing the picture, how are Recombinant DNA formed? What is the difference between genetic…
A: Introduction: Genetic engineering or genetic modification or genetic manipulation involves direct…
Q: ripe banana a better sample compared to an unripe banana for DNA extraction
A: Raw bananas are often preferred for DNA extraction because they have high number of cells per…
Q: suppose there three tubes containing DNA and RNA and protein sample in the laboratory. the three…
A: DNA(deoxyribonucleic acid) is defined as the building block of life because it carries all the…
Q: Which of the following most accurately describes the process of DNA cloning? set of laboratory…
A: Cloning requires DNA with desirable genes obtained using restriction enzymes which is then…
Q: w many DNA molecules would exist after 30 PCR cycles?
A: The PCR reaction is one of the breakthroughs in the field of biotechnology. The discovery of PCR has…
Q: Amplifying environmental DNA at low copy numbers is subject to contamination of DNA signatures from…
A: PCR is the process by which scientists can detect contamination DNA.
Q: Briefly explain the rationales of adding chemicals that can affect DNA stability in a polymerase…
A: Ans: Polymerase Chain Reaction (PCR): The in-vitro amplification of DNA by using primers, dNTPs, taq…
Q: Which ingredient will allow the DNA to form cloudy clumps which can be collected with tweezers salt…
A: Introduction :- A given DNA segment can be quickly multiplied (amplified) into millions or billions…
Q: The ingredients used in PCR will contain all of the following except: Group of answer choices A.…
A: The PCR or Polymerase chain reaction is a widely preferred laboratory technique which is used to…
Q: what are the difference of classical breeding and recombinant dna technology
A: Classical Breeding: Classical breeding, which entails selectively breeding the best-performing…
Q: If Susie collects her cheek cells, but accidentally dumps the cell pellet into the waste container…
A: A cell pellet is prepared from human primary cells and it is utilized in polymerase chain reaction.
Q: The Southern blot procedure was developed so that: DNA fragments that have been subjected to…
A: Southern blotting is the technique for the detection of specific DNA fragments. The detailed…
Q: double-stranded length of DNA is exposed to a restriction enzyme. The enzyme finds 3 recognition…
A: Restriction enzymes Restriction enzymes are the specific enzymes that cleave the DNA in very…
Q: For the isolation of DNA from buccal cells using saline solution, concentrated soap, and ice cold…
A: For isolating DNA from buccal cells. First, we need buccal cells which we are getting…
Q: What implication does eating DNA have on the commercial properties of DNA in your hair conditioner,…
A: As Humans have continuously consumed deoxyribonucleic acid from plants and animals.
Q: g best describes the complete sequence of steps occurring during every cycle of PCR? I-The primers…
A: A laboratory method used to make many copies of a specific piece of DNA from a sample that contains…
Q: In which sequencing method, DNA is labelled and then chemically cleaved in a sequence-dependent…
A: DNA sequencing refers to decoding the base sequence of the DNA strand. It tells us the base…
Q: An individual’s unique set of_______ can be used in DNA profiling. a. DNA sequences c. SNPs b. short…
A: Introduction In the genome, there is usually two types of nucleic acid sequence present. One is…
Q: The following statements are true about common gene cloning procedures except: -DNA plasmids can…
A: Biotechnology is a branch of biology, including the use of living organisms to produce products.…
Q: Answer the correct options do not need expl
A: PCR is called as the polymerase chain reaction. The materials in the PCR reaction includes the…
Q: PCR is a powerful technique to screen and amplify segments of DNA for use in recombinant protein…
A: The polymerase chain reaction is abbreviated as PCR. It is a molecular biology technique for…
Q: Some recombinant DNA techniques depend on the specific hybridization (or annealing) between two…
A: The invention of recombinant DNA technology was carried out by Herbert Boyer & Stanley & N.…
Q: For DNA amplification using PCR to occur, which of the following are needed? A. DNA primers…
A: The term polymerization stands for the production of a large chain or network-like molecules, from a…
Q: The following individuals contributed to the discovery of DNA. Research. what their contribution(s)…
A: Deoxyribonucleic acid (DNA) stores the cell’s genetic information and is present in the nucleus of…
Q: Humans have very similar DNA sequences, with approximately 999/1000 letters being One possible way…
A: The human genome is a complete collection of nucleic acid sequences for humans, encoded as DNA in…
Q: Describe at least one important application of DNA technology in each of the following fields:…
A: These days, DNA-based technology is very common. Biotechnology is the process of manipulating living…
Q: can you explain these statement and why molecules of dna from two different species that are…
A: DNA is the molecular name for the molecule in all living things that convey genetic instructions.…
Q: What is the purpose of genetic engineering/DNA recombinant technology? How can this technique…
A: Genetic engineering is a technology that introduces foreign genes into plants, microbes, and animals…
Q: Diagram and describe the methods of PCR, DNA sequencing, Restriction Enzymology, & Hybridization…
A: A gene is the essential physical and functional unit of heredity. Genes are comprised of DNA. A…
Q: Describe the whole process and the principle behind DNA extraction.
A: Deoxyribonucleic acid (DNA) is a polymer made up of two polynucleotide chains that coil around each…
Q: What are the different methods of quantifying DNA? How would you determine if your
A: Quantitation of DNA ,methods used for quantifying DNA ---* Quantifying of DNA -- In various methods…
Explain some of the evidence that supports the identity of DNA as hereditary material. Make sure to include a couple of experiments.
Step by step
Solved in 2 steps
- Describe at least two safety issues associated with DNA technologyand explain how these issues are being addressed.1 2 Today's technology has made it easier to quickly and accurately generate DNA profiles. In this part of the activity, you will model the process yourself to solve a crime. Good luck, detective! Crime Report: A thief has stolen a priceless collection of jewels from the Museum of Precious Jewels. Forensic technicians obtained skin cells from a forehead print left on the glass enclosure of the jewel exhibit. DNA has been isolated and PCR amplified for some of the standard STR loci. A partial genetic profile generated from the collected DNA is shown in Figure 5. 10 50 DNA Profile from Forehead Print Number of base pairs 00 50 40 D58818 075830 I 16 MU DES1179 Shandand (10) 70 CSF1PO DITS820 80 100 Figure 5. The DNA profile of the forehead print from the scene of the crime. Each colored line shows the alleles for one of four of the core CODIS STR loci (D5S818, CSF1PO, D7S820, D8S1179). and data for the four STR loci that were included in the A suspect was identified in the case. Her DNA…Molecular biologists rely on many, often sophisticated, techniques to pursue their discipline. One may list ultracentrifugation, electron microscopy, X-ray diffraction, electrophoresis, and computer interfacing as fundamental tools. Model organisms provide the raw materials for study. List three "organisms" (or organismic groups) often used by recombinant DNA technologists and describe a major advantage of each group.
- A scientist made mistakes during DNA extraction. On his first attempt, he blended the sample too much for a long time. On his 2nd attempt, he used potassium acetate instead of sodium chloride. On his third attempt, he forgot to put C4H8O2.1. Which attempt would be the highest based on increasing DNA purity? what about by increasing DNA yield?2. Explain the effect of his mistakes in each of his attempts on the yield and purity of the DNA sample extracted.3. Which attempt would be the most successful? Why?The development of DNA technology is bringing profound changes to science, agriculture and healthcare. Provide one example of a DNA technology and provide at least one advantage and one example of a concern or problem associated with its use.Please answer these two questions regarding PCR: a) Why do you need to perform PCR on DNA obtained from a crime scene? b) Why so forensic labs analyze non-coding DNA rather than genes?
- Describe at least one important application of DNA technology in each of the following fields: medicine, DNA fingerprinting, and transgenic organisms.Explain the properties of DNA that lend it to manipulation in the laboratory.Answers must be precise and concise. You can use other sources of information related to the topic. QUESTION: How will knowledge of recombinant DNA technology be useful to resolve the current challenges and issues we are facing today? Cite an example.
- What is the application of DNA extraction in healthcare? Cite at least 3 specific examples.Which of the following is the correct order in extracting the DNA? I. Washing of DNA II. Tissue homogenization and cell lysis III. Drying of pellet and dissolution of dried pellet IV. Precipitation of DNA from the aqueous phase V. Denaturation and separation of other biomolecules from DNAThe last steps of DNA extraction include washing it using ethyl alcohol and drying the DNA pellet. Why do we need to wash the DNA using ice-cold ethyl alcohol? And why do we have to dry the DNA pellet?
