CGATTGCAGGTTATAGCG. Which of the following primer pair can be used to amplify this template with PCR? a. GCTAA and TAGCG b. GCTAA and CGCTA c. CGATT and TAGCG d: CGATT and CGCTA

a. What is the purpose of molecular cloning?b. What purpose do selectable markers serve in vectors?c. What is the purpose of the origin of replication in aplasmid vector?d. Why do cloning vectors have polylinkers?

What specifically will happen if DNA polymerase is inaccurate during DNA synthesis? Explain how this inaccuracy might affect the organism.

You are performing a PCR reaction but unbeknownst to you, there is a significant pool of dUTP in the nucleotide mix (along with dCTP, dTTP, dATP, and dGTP). How might this affect your PCR product? a. If the PCR product was ligated into a plasmid and put into a cell, a totally different mRNA would be made from the insert compared to an insert made with T's.  b. If the pool of dTTP ran out before the pool of dUTP, DNA replication could no longer occur.  c. During the reaction, uracils incorporated into the product would cause the PCR product to degrade as it is being made.  d. Uracil would be incorporated into the product and would lessen the affinity of any DNA binding proteins that might bind to the product in subsequent experiments. e. Nothing would happen since polymerases can't use dUTP to make DNA.