4. 1 71 141 211 281 351 421 491 561 631 701 771 841 911 981 Below is the full sequence of the 16S ribosomal DNA (1457 bp) for Coxiella burnettii, as well as a section of the flanking DNA sequence (lower case letters): ATTGAAGAGTTTGATTCTGGCTCAGATTGAACGCTAGCGGCATGCTTAACACATGCAAGTCGAACGGCAG CGCGGGGAGCTTGCTCCCTGGCGGCGAGTGGCGGACGGGTGAGTAATGCGTAGGAATCTACCTTGTAGTG 1121 GGGGATAACCTGGGGAAACTCGGGCTAATACCGCATAATCTCTTTGGAGCAAAGCGGGGGATCTTCGGAC CTCGTGCTATAAGATGAGCCTACGTCGGATTAGCTTGTTGGTGGGGTAATGGCCTACCAAGGCGACGATC CGTAGCTGGTCTGAGAGGACGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAG CAGTGGGGAATATTGGACAATGGGGGAAACCCTGATCCAGCAATGCCGCGTGTGTGAAGAAGGCCTTCGG 70 140 210 280 350 420 490 560 630 GTTGTAAAGCACTTTCGGTGGGGAAGAAATTCTCAAGGGTAATATCCTTGGGCGTTGACGTTACCCACAG AAGAAGCACTGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGAGTGCAAGCGTTAATCGGAATCAC TGGGCGTAAAGCGCGCGTAGGTGGATATTTAAGTCGGATGTGAAAGCCCTGGGCTTAACCTGGGAATTGC ACCCGATACTGGGTATCTTGAGTATGGTAGAGGGAAGTGGAATTTCCGGTGTAGCGGTGAAATGCGTAGA 700 TATCGGAAAGAACACCAGTGGCGAAGGCGACTTCCTGGACCAATACTGACACTGAGGCGCGAAAGCGTGG 770 GGAGCAAACAGGATTAGAGACCCTGGTAGTCCACGCCGTCAACGATGAGAACTAGCTGTTGGGAAGTTCA 840 CTTCTTAGTAGCGAAGCTAACGCGTTAAGTTCTCCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAA GAAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAAAACCTTACC TACCCTTGACATCCTCGGAACTTGTCAGAGATGATTTGGTGCCTTCGGGAACCGAGTGACAGGTGCTGCA 910 980 1050 1120 1051 TGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACCCTCGTCCTTAGT TGCCAGCGAGTCAAGTCGGGAACTCTAAGGAGACTGCCGGTGATAAACCGGAGGAAGGTGGGGATGATGT 1190 CAAGTCATCATGGCCCTTACGGGTAGGGCTACACACGTGCTACAATGGGCAGTACAAAGGGTTGCCAAGC 1191 1260 1261 CGCGAGGTGGAGCTAATCCCAGAAAACTGCTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAG 1330 1331 TTGGAATCGCTAGTAATCGCGAATCAGCATGTCGCGGTGAATACGTTCTCGGGCCTTGTACACACCGCCC 1400 1401 GTCACACCATGGGAGTGAATTGTACCAGAAGCGGGTAGGCTAACCTTCGGGAGGCCGctcaccacggtat 1470 1471 gatccatgactggggtgaagtcgtaacaaggtagccgtaggggaacctgcggctggatcacctccttact 1540 1541 tttagtgatccaatacttgtaccggtatccacacaaactgctttcttaatttttaaaggatccaggatct 1610 Identify the position of the four primers from Wilson et al. (1990) listed below on the Coxiella rDNA sequence. Highlight the recognition sequence in colour, and also indicate with an arrow the direction in which each primer will amplify the fragment during PCR. (NB: READ the footnote to the table! Make sure you know the difference between a forward and a reverse primer!) Primer Pomod P3mod PC3mod Sequence and arrow colour BLUE GREEN PURPLE PC5 RED