You want to amplify a specific region of DNA during PCR. Your primers should

Think about the function of the primers in a PCR that is designed to amplify a specific sequence, and about the temperatures of the PCR cycle. After considering these two points, give two reasons why 4-nucleotide long primers are not appropriate for PCR and explain.

In a PCR assay, what's the purpose of each temperature step at: -heating the sample to 50 C -heating the sample to 72 C -heating the sample to 95 C options to match: - allows primers to anneal to the template DNA - promotes synthesis of new DNA - separates the DNA strands in the samples

fellow lab worker brings you DNA containing what might be a similar gene in Leopard Geckos (XG). She asks you to see if you can amplify it using the same primers you used in frogs and the Bearded Dragon. You run the PCR and then analyze the product by running the DNA on an agarose gel. As a control, you run out the PCR product you amplified in Bearded Dragons and a DNA “ladder” of DNA pieces of known sizes. The gel results are shown below. Marker = DNA ladder; Lane A = Bearded Dragon PCR product; Lane B = Leopard Gecko PCR results. In the box below, determine the sizes of the PCR products shown in Lanes A & B. Size of Bearded Dragon PCR product: ______________________________  Sizes of Leopard Gecko PCR products: ______________________ yes or No - based on the results shown in the gel above – would you say the frog PCR primers for gene X amplified the same gene in the Bearded Dragon?