In regards to using PCR: Explain why a plasmid is often engineered with tetR and lacZ. What purpose do they serve?

Identify mutant genes by plasmid library transformation?

Using the Figure below briefly describe how the amplification can be used in the cloning process of DNA.

A plasmid contains genes for ampicillin resistance and chloramphenicol resistance and has single sites for Hind III and Eco RI. When genes are inserted into the Eco RI site, transformants are resistant only to ampicillin. In which region is the site for EcoRI  located? Group of answer choices within the chloramphenicol gene between the ampicillin and chloramphenicol genes can't be determined within the ampicillin gene

The modifiedplasmid is reintroduced back into Rhizobium(step 4) and the genetically transformed bacteria are then selected based on the amp and lacZgenes present within the plasmid. The plasmid may or may not integrate the BBW resistancegene. The treated bacteria may or may not take up the modified plasmid.   a) Complete the table below with a yes or no in each space stating whether you would expect these bacteria to grow or not. Type of treated bacteria culture plate(no amp) Culture plate treated with ampicillin   Plasmid not taken up     Plasmid taken up (WithoutBBW resistancegene)     Plasmid taken up (WithBBW resistancegene)     B) Outline how the genetically transformed bacteria containing the BBW resistance gene can beselected based on the amp and lacZgenes present within the plasmid.

A plasmid has been modified such that it carries the gene for beta-galactosidase (lacZ) under the control of the lac promoter. Between the lac promoter and the lacZ gene is an empty multiple cloning site (MSC). This plasmid also carries ampicillin resistance. E. coli are transformed with this plasmid, and grown on nutrient agar containing ampicillin, IPTG, and the lactose analogue Xgal for 18 hours. Following incubation, the plates are examined. What will be the likely colour of the bacteria colonies?

What are plasmids? What is the importance of plasmids for the recombinant DNA technology?