Calculate the pH of the following buffers containing; 2.95M HC2H3O2 and 1.5M NaC2H3O2 ka = 1.8X10 ka = 6.8X104 а. b. 3.0MHF and 2.5M NaF %3D
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- &. How many milldiners of a 2wv Lidocaine HCI stock selution in water should be used in preparing 30 ml of a 0.5% wiv Lidocaine HCI solution? . From the following formula for antificial tears, caleulate the quantity of each ingrodient roquired to prepare half donen of the 0ml containers. Poyviny alcohol 149 Povidone Chiorobutanol Serile sodum chioride soution a.%. s to 100 m How many milldiners of water should be added to 60 ml. of a 2% wiv agqueous solution to prepare a 1% wiv solution?Ib 20 n moles OL produch ane observed In 10 minutes and 50 nmoles 20 minutes obsenved is Vo? product in are then cwhatOn the right the Hill plot com- (b) pares the O2 binding properties of Hb Ya- kima with those of HbA in 0.1 M NaCl buff- Hb-Yakima ered to pH 7 with 0.01 M bis-Tris. Focus first on the line for "stripped Hb". This is the term for hemoglobin isolated from erythro- cytes with removal of all organic phosphate molecules that might bind to the protein in RBCS. You can see that 2,3-bisphospho- glycerate (BPG; labeled DPG according to old terminology) does not alter the O2 bind- ing affinity of Hb Yakima in contrast to HbA (although it was shown that BPG did bind to the deoxyHb Yakima molecule). Also, Hb Yakima is associated with markedly decreased allostery in the absence and presence of BPG, in comparison to HbA. IHP = inositol hexaphosphate, an artificial allosteric modifying ligand that binds more tightly than BPG. stripped Hb Hb-A •DPG +DPG n= 1.0 n = 2.3 n=2,5 +THP +IHP 0.5 0.5 1 5 10 50 po, ( mm Hg ) %3D On the right is a diagram copied from the lecture handout "Hemoglobin and Allo-…
- Effect of pH on Microbial Growth 12 10 8 Escherichia coli -Pseudomonas aureus -Staphylococcus aureus -Listeria monocytogenes 1 4 pH Minimum Optimum Maximum Escherichia coli 4.5 7 Pseudomonas aureus 4.5 7.5 9.5 Staphylococcus aureus 6.8 Listeria monocytogenes 4.5 7 9.6 Make a discussion on the experiment of the effect of pH on microbial growth. Growth Rate of Microbial 2.Beef extract 3.0 g 10.0 g Pancreatic digest of casein 5.0 g NaCl Agar (low percentage to allow for visualizing swimming motility) 4.0 g 1.0 L Water O Defined O Complex O Minimal Selective DifferentialO Ara (Alt+ Ğ) Yeni. - Gözden Geçir Görünüm Yardım Başvurular Posta Gönderileri 1) Discuss this graph in the meaning of substrate depletion. 100 80 [S] [P] 60 40 20 20 40 60 80 100 Time (s.) ere goz atın 7SUS 17 19 5 8. 9. Relative Concentration
- 5.15. The following data were obtained when glucose (C6H₁2O6) was added to a batch culture of microorganisms. Determine the reaction order for the disappearance of glucose. TIME. min 0 10 20 30 40 50 CONCENTRATION, g/m³ Glucose Cells 100 67 50 40 33 29 1500 1516 1525 1530 1534 1535Which of the following substances does not participate in the CalvinBenson cycle? a. ATP b. NADPH c. RuBP d. PGAL e. O2 f. CO210 If3 ml of culture is diluted by adding 9 ml of water and the absorbancy reading of the diluted culture is 0.082, what is the actual absorbancy?
- can you help me with this? 1.- calculate the concentrations of all the species present with a 0.10 M EDTA solution (considering all equilibria) 2.- Write down the amounts of reagents that you would use to prepare 760 ml of a carbon-based buffer with a pH of approximately 10.4 and a concentration of 0.125 MComposition of Cell Lysis Buffer Required Concentration of Reagent Molecular Weight Volume or Weight of Reagents Needed 40 mM Tris HCl 157.59 20 mM Acetic acid 60.05 1 mM EDTA 292.24 10 mM MgCl2 from 25mM stock 95.21 5% SDS 288.37 ddH2O 18.02Chitinase is a protein that breaks down chitin, a primary component of the cell wall in fungi, scales in fish and exoskeletons of arthropods. The activity of chitinase extracted from a plant was shown to be optimum at pH 5. You were tasked to prepare 300 mL of 150 mM buffer solution for further analysis of the extracted chitinase. REAGENTS Ka 2.5M Acetic acid Solid NaOAc•3H2O [136.08g/mol] 1.76 x 10-5 2.5M NH3 Solid NH4Cl [53.49g/mol] 5.6 x 10-10 2.5M Lactic acid Solid sodium lactate [112.06g/mol] 4.0 x 10-5 5 M HCl 5M NaOH Pls show sol'ns 1. Given the following reagents, give the moles of each component (acid & base).2. What are the mass/volume of the components needed to prepare the buffer? 3. What will the pH of the buffer be if 1mL of 5 M NaOH was added?
