Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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Composition of Cell Lysis Buffer
Required Concentration of Reagent | Molecular Weight | Volume or Weight of Reagents Needed |
40 mM Tris HCl | 157.59 | |
20 mM Acetic acid | 60.05 | |
1 mM EDTA | 292.24 | |
10 mM MgCl2 from 25mM stock | 95.21 | |
5% SDS | 288.37 | |
ddH2O | 18.02 |
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