Below is a Beer's Law Plot for the concentration of a specific colored compound x. A solution of compound X is measured an optical density of 0.60. a) What is the concentration of compound X in this solution. Answers must contain proper units for full credit. b) The absorbance of this compound was measured at 540 nm. Why was this absorbance chosen? D.R O.R 0.7 0.6 805 orbance at 540

Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter19: Biomedical Laboratory Instrumentation
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Below is a Beer's Law Plot for the concentration of a specific colored compound x. A solution of compound X is measured with the spectrophotometer to have
an optical density of 0.60.
a) What is the concentration of compound X in this solution. Answers must contain proper units for full credit.
b) The absorbance of this compound was measured at 540 nm. Why was this absorbance chosen?
0.9
0.8
0.7
0.6
805
4
0.4
0.3
0.2
Absorbance at 540 m
Transcribed Image Text:Below is a Beer's Law Plot for the concentration of a specific colored compound x. A solution of compound X is measured with the spectrophotometer to have an optical density of 0.60. a) What is the concentration of compound X in this solution. Answers must contain proper units for full credit. b) The absorbance of this compound was measured at 540 nm. Why was this absorbance chosen? 0.9 0.8 0.7 0.6 805 4 0.4 0.3 0.2 Absorbance at 540 m
Absorbance at 540 m
09
O.R
0.7
80.5
0.4
0.3
0.2
0.1
0
0.5
1.5.
2
Concentration (micromoles/ml)
For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac).
1
2.5
3.5
Transcribed Image Text:Absorbance at 540 m 09 O.R 0.7 80.5 0.4 0.3 0.2 0.1 0 0.5 1.5. 2 Concentration (micromoles/ml) For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac). 1 2.5 3.5
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