Below is a Beer's Law Plot for the concentration of a specific colored compound x. A solution of compound X is measured an optical density of 0.60. a) What is the concentration of compound X in this solution. Answers must contain proper units for full credit. b) The absorbance of this compound was measured at 540 nm. Why was this absorbance chosen? D.R O.R 0.7 0.6 805 orbance at 540
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- The standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.Guanosine (C10H13N5O5) in solution has a maximum absorbance at a wavelength of 275 nm. The molar extinction co-efficient at this wavelength is 84M−1cm−1and the path length is 24.7 cm. Through the use of a spectrophotometer, it is found that the that A275= 1.48. What is the concentration of the guanosine solution in grams/litre? Molecular weights (g/mol): C-12, H-1, N-14, O-16 Select one: A. 0.201869 g/L B. 0.435188 g/L C. 0.059919 g/L D. 0.294046 g/L E. 0.000713 g/LThis is a question regarding spectrophotometry. Scenario: 0.1 mL of the unknown and 0.9 mL of distilled water are mixed in a cuvette and the absorbance is measured at 280 nm. Given the absorbance, molecular mass and the extinction coefficient of the unknown are 0.253, 16950 g and 13940 M-1cm-1 respectively, and the path length is 1 cm, how can I find the concentration of the unknown in mg/mL by Beer-Lambert Law (NOT molarity M)?
- A chemist is performing a precipitation titration on a 1.00 L sample containing an unknown concentration of the cadmium ion, Cd2+. If it takes 24.52 mL of a 0.250 M solution of Na2CO3 to reach the end point, calculate the concentration of the cadmium ion?1.0 ml of serum albumin (BSA) solution was precisely diluted to 100 ml with a buffer solution, and the absorbance at 280 nm was measured from this buffer solution at a distance of 1 cm from the light. The result was A=1.0. BSA is 0.1%(=0.1g/l). The absorptivity is e(0.1%)=0.667, so 1g/l solution gives an absorbance of 0.667. What was the original protein content? Report in the result unit mg/ml with an accuracy of 0.1 mg.A drug has a measured diffusivity of 5*10^-6 cm^2/s^-1 at 37 degree Celsius. What is this drug’s Stokes-Einstein radius in nm?
- Four known amino acids (AA) produce a chromatograph with Rf values as follows: AA1 =0.25, AA2 =0.66, AA3=0.50,AA4=0.80. A mixture of three amino acids yields spots that are 2 cm,5.2cm, and 6.4 cm from the organ. The solvent front is 8.0 cm from the origin. Which three of the four amino acids are contained in the mixture?Calculate the pH of the resulting solution if 23.0 mL of 0.230 M HCl(aq) is added to 33.0 mL of 0.230 M NaOH(aq). pH = Calculate the pH of the resulting solution if 23.0 mL of 0.230 M HCl(aq) is added to 13.0 mL of 0.330 M NaOH(aq). pH=1) A solution is made by combining 128 mL of ethanol and 0.452 L of water. In this solution, what is the solvent? What is the solute?2) If a solution contains 128 mL of ethanol and water to make 0.452 L of solution, what is the (v/v)% concentration?3) How many grams of sodium carbonate are needed to make 350 mL of a 0.5% (w/v) sodium carbonate solution?4) What is the molarity of a magnesium chloride solution made by dissolving 8.7g of magnesium chloride in 2.45 L of water?5) An exercise in lab requires you to make 500 mL of a 2.3M beryllium sulfide solution. How many moles of beryllium sulfide will you need?6) How many grams of sodium chloride are needed to make 0.60L of a 2.3M sodium chloride solution?7) How hot must the air in a balloon be heated if initially it has a volume of .750 L at 20°C and the final volume be 1.00 L?8) Convert 455 mmHg to atm.9) A container of gas has an initial pressure of 0.8 atm. The pressure is increased to 1.1atm and the final volume of gas is 2.3 liters.…
- A chemist combined chloroform (CHCI,) and acetone (C, H,O) to create a solution where the mole fraction of chloroform, Xchloroform, is 0.187. The densities of chloroform and acetone are 1.48 g/mL and 0.791 g/mL, respectively. Calculate the molarity of the solution. Assume the volumes are additive. molarity: Calculate the molality of the solution. molality: mA glucosamine solution of unknown concentration was diluted as follows: 1 mL of the solution was diluted to 5.0 mL. A 0.3 mL aliquot of this solution was diluted with 0.7 mL of water; the absorbance of this was measured and found to be 0.440 (uncorrected, must subtract blank). What is the concentration of the original undiluted glucosamine hydrochloride solution in µmol/mL? Given: Corrected absorbance = 0.395 y= 1.165x+0.055 Standard equationIf you have an absorbance of 2.0 mL of stock dye, what kind of a dilution is needed to get it to 1.0? How do you go about this dilution and how do you calculate?