2. 500 ml of a 0.1 M sodium phosphate buffer with pKa = 7.21, is diluted a) How would you dilute this buffer 100 times to make a final volume of 50 ml? Show the ratios to be added together. b) Calculate the pH of the buffer when it is diluted with dH20 c) Calculate the pH of the buffer when it is diluted with 0.3 M KCI 3. What is the normality of a 5 M phosphoric acid (SG = 1.88 g/cm3; 85% solution has a Mr of 98 g/mole %3D
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- 1) Calculate the required gram of Tris-Hcl to prepare 1M, 100 ml Tris-HCl (Tris Stock) with mol and Molarity formula. 2)Calculate the required gram of EDTA to prepare O.5M, 100 ml EDTA (EDTA stock) mol and Molarity formula. 3.) Calculate the required volume of Tris Stock to prepare buffer A which contains 25 mM Tris-Hcl with final volume is 100 ml by using M1V1 =M2V2 formula 4) Calculate the required volume of EDTA stock to prepare buffer A which contains 10 mM EDTA with final volume is 100 ml by using M1V1 =M2V2 formula 5)Do the same for Buffer B calculationsWhat is the final concentration if 239mL of a 3.9M glucose solution is diluted to a volume of 842mL?Draw titration curve for compound X when NaOH equivalence(s) is/are added. Indicate the buffering region(s) with a box.
- 3 mL of a 45 mM stock solution of a substrate is added to 8 mL of water. Calculate the following values. The substrate molecular weight is 125 g/mol. Calculate the following values: Substrate Volume in mL Dilution Factor Substrate number of moles Substrate concentration for the diluted solution in mM Substrate concentration for the diluted solution in mole/L Substrate concentration for the diluted solution in mg/mLWhat mass of sodium glycolate (NaC2H3O3) should be added to 400.0 mL of 1.00 M glycolic acid to produce a buffer solution with a pH of 4.00? Ka = 1.47 x 10-4. Please indicate the full solutions.Consider a HCI solution with a pH of 4.70. a) Calculate the concentration of HCl. b) Identify the two underlying assumptions in part a). c) The original solution is diluted by 1,000 fold. Calculate the pH of the resulting solution. Make sure to indicate if the forementioned assumptions are still valid.
- At what pH does a lysine solution exhibit the highest buffering capacity? (Lysine pKas: pk1 = 2.2, pK2 = 8.95, pK3 = 10.5) %3D O рH 12.1 О рH 9.72 O pH 5.67 pH 8.95It takes 175ml of 0.75 M H2SO4 to completely neutralize 285 ml of NaOH. What is the concentration of the NaOH solution?You need to prepare an acetate buffer of pH 5.43 from a 0.621 M acetic acid solution and a 2.95 M KOH solution. If you have 730 mL of the acetic acid solution, how many milliliters of the KOH solution do you need to add to make a buffer of pH 5.43? The pKa of acetic acid is 4.76. Be sure to use appropriate significant figures.