To observe T. spathacea cells, a sharp blade was used to peel off a thin portion of the lower epidermisof the leaf and was placed on a clean glass slide. One to two drops of 0.01 M NaCl solution was added and a coverslip was placed. The procedure was repeated using 0.30 M NaCl. The leaves were examined under LPO and HPO. A portion with only a single layer of cells was observed. Cells under HPO are shown in your worksheet. Give a short description of the T. spathacea leaf cells in 0.01 M and 0.30 M NaCl solutions. In the figures, label the cell wall, vacuole and tonoplast. Be sure to show the differences between the two leaves and describe them. Notice if the anthocyanin moved out of the vacuole. ## 2.2. Responses of *Tradescantia spathacea* Leaf Cells to Solutions of Different Osmotic Concentrations### Figure 4.3a.*T. spathacea* leaf cells in 0.01 M NaCl (Magnification: 400X)**Description:** - The image shows *Tradescantia spathacea* leaf cells exposed to a low concentration of NaCl solution.- Cells appear turgid, maintaining their structure and filled with cytoplasm.- The cell walls are clearly defined, and there is little space between them.### Figure 4.3b.*T. spathacea* leaf cells in 0.30 M NaCl (Magnification: 400X)**Description:** - The image depicts *Tradescantia spathacea* leaf cells in a higher concentration of NaCl solution.- Cells appear plasmolyzed, with cell membranes pulled away from the cell walls.- There are noticeable gaps between the cell membrane and the cell wall, indicating water loss.- The structure of the cells is less rigid compared to the lower concentration solution.

Introduction Plant cells maintain the Turgor pressure in order to control the cell shape. Turgor…

Answered: ## 2.2. Responses of *Tradescantia…

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To observe T. spathacea cells, a sharp blade was used to peel off a thin portion of the lower epidermis
of the leaf and was placed on a clean glass slide. One to two drops of 0.01 M NaCl solution was added and a coverslip was placed. The procedure was repeated using 0.30 M NaCl. The leaves were examined under LPO and HPO. A portion with only a single layer of cells was observed. Cells under HPO are shown in your worksheet. Give a short description of the T. spathacea leaf cells in 0.01 M and 0.30 M NaCl solutions. In the figures, label the cell wall, vacuole and tonoplast. Be sure to show the differences between the two leaves and describe them. Notice if the anthocyanin moved out of the vacuole.

## 2.2. Responses of *Tradescantia spathacea* Leaf Cells to Solutions of Different Osmotic Concentrations

### Figure 4.3a.
*T. spathacea* leaf cells in 0.01 M NaCl
(Magnification: 400X)

**Description:**
- The image shows *Tradescantia spathacea* leaf cells exposed to a low concentration of NaCl solution.
- Cells appear turgid, maintaining their structure and filled with cytoplasm.
- The cell walls are clearly defined, and there is little space between them.

### Figure 4.3b.
*T. spathacea* leaf cells in 0.30 M NaCl
(Magnification: 400X)

**Description:**
- The image depicts *Tradescantia spathacea* leaf cells in a higher concentration of NaCl solution.
- Cells appear plasmolyzed, with cell membranes pulled away from the cell walls.
- There are noticeable gaps between the cell membrane and the cell wall, indicating water loss.
- The structure of the cells is less rigid compared to the lower concentration solution.

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