1a)A surface sterilisation protocol was carried out to sterilise leaf and stem explants for evaluating the efficacy of the protocol. All explants were washed thoroughly with distilled water before treating with 50% Clorox for 20

An Illustrated Guide To Vet Med Term

4th Edition

ISBN:9781305465763

Author:ROMICH

Publisher:ROMICH

Chapter15: Feed And Protect Me

Section: Chapter Questions

Problem 14MC

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Question

1a)A surface sterilisation protocol was carried out to sterilise leaf and stem
explants for evaluating the efficacy of the protocol. All explants were washed
thoroughly with distilled water before treating with 50% Clorox for 20
minutes. The explants were then rinsed with distilled water before the explants were cultured on plates containing Murashige and Skoog (MS) basal medium. A total of five plates were prepared for each type of explant and each plate consisted of 10 explants. The results recorded after a week are shown in Table. Discuss and conclude the results obtained.

Table 1: Results of surface sterilisation obtained for leaf and stem explants
Number of
Explant
Contaminated
Number of
Explant
Survived
Leaf
Stem
Leaf
Stem
Plate 1
Plate 2
Plate 3
2.
7
3
1.
8.
2.
2
6.
Plate 4
1.
Plate 5 1
8.
7
6.
6.
906

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