A researcher sequenced a portion of a bacterial gene and obtainedthe following sequence, beginning with the start codon, which isunderlined:ATG CCG GAT TAC CCG GTC CCA AAC AAA ATG ATCGGC CGC CGA ATC TAT CCCThe bacterial strain that contained this gene has been maintainedin the laboratory and grown serially for many generations. Recently,another person working in the laboratory isolated DNA from thebacterial strain and sequenced the same region. The followingresults were obtained:ATG CCG GAT TAT CCG GTC CCA AAT AAA ATG ATCGGC CGC CGA ATC TAC CCCExplain why the differences in the sequences may have occurred.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
A researcher sequenced a portion of a bacterial gene and obtained
the following sequence, beginning with the start codon, which is
underlined:
ATG CCG GAT TAC CCG GTC CCA AAC AAA ATG ATC
GGC CGC CGA ATC TAT CCC
The bacterial strain that contained this gene has been maintained
in the laboratory and grown serially for many generations. Recently,
another person working in the laboratory isolated DNA from the
bacterial strain and sequenced the same region. The following
results were obtained:
ATG CCG GAT TAT CCG GTC CCA AAT AAA ATG ATC
GGC CGC CGA ATC TAC CCC
Explain why the differences in the sequences may have occurred.
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