The following bacterial DNA genome is composed of three sequences: TGGCGGT / ACGTTAC / GGATAGG Using your knowledge of CRISPRs, identify which of these sequences is/are most likely derived from viral DNA infection.   a. TGGCGGT   b. ACGTTAC   c. GGATAGG   d. Both a and c

Design PCR primers for the DNA sequence given below and explain your choice. As part of your answer, draw a schematic of PCR primers binding to the DNA template. 5'-ATGGGGTCAGTCATGGTTA... .TTTATCGTGCATGTGATGTG-3'

For the following sequence of amino acids, serine-valine-lysine-leucine, which of the choices below is the correct order for the nucleotide base sequence in DNA? Group of answer choices a. UGUGCAAAGUUA b. AGACAATTCAAT c. TCTCGTTTGTTA d. TGTGCTTTCTTA

Please answer both parts, a. The enzyme that catalyzes the joining of fragments to form recombinant DNA is: Question 21 options: DNA polymerase DNA ligase helicase restriction endonuclease b. In the above diagram, ‘1’ represents the Question 3 options: sticky ends restriction sites primer restriction fragments

This individual's x-ray diffraction data helped to confirm that DNA was orientied in a helical structure with uniform diameter. O a. Rosalind Franklin b. James Watson O c. Francis Crick O d. Gregor Mendel e. Edwin Chargaff

Use the set of gene sequencing results below to answer the question that follows: A G C T -Wells I 14. Based on the sequencing results above, what is the sequence of nucleotides as they were added by DNA polymerase to the DNA template? a/ATC-GCA-GTA b. TAG-TGC-CAT c. TAC-TGC-GAT GC-GA TAG-CGT-CAT L A EI la la اد ان YI

Restriction endonucleases are bacterial enzymes that cleave duplex (double-stranded) DNA at specific nucleotide sequences. The mode of replication of the animal virus SV40 has been investigated by using restriction endonucleases that cleave SV40 DNA into a number of unique segments. Like most viruses, SV40 DNA is circular. The map positions of the 11 fragments produced by a pair of restriction endonucleases are shown on the next page. Immediately following a 5 or 10 minute pulse of radioactively labeled thymidine, labeled SV40 molecules that have completed replication during the pulse are isolated. These newly replicated DNA molecules are digested by the restriction endonucleases and the resulting fragments are analyzed for the relative amounts of pulse label they contain. The results are in the table below. Assume that at the time the label was added there was a random population of replicating SV40 DNA molecules in all possible stages of synthesis. From the information given below,…

Answer the following questions about CRISPR below: A.What is a PAM sequence? B.How does the Cas-9/RNA sequence target a piece of DNA for destruction? C.How is the DNA repaired after being cut by CRISPR/Cas-9?

72) In case of RNA dependent RNA polymerase in Newcastle disease virus (NDV), is a a. Its template is Single stranded DNA b. Its template is Single stranded RNA c. Its product is Double stranded DNA d. Its product is Double stranded RNA

A mutant DNA strand was transcribed then translated to proteins. a. What is the protein product of the mutant DNA strand? The sequence of the mutant strand is shown below: 5'-TGCCATAACTGTTCGTACTGGCAAATTGCC-3' 3'-ACGGTATTGACAAGCATGACCGTTTAACGG-5' b. The mutation altered the sequence of the wild type template DNA such that a degenerate codon for a basic amino acid in the wild type was converted to a non-degenerate codon resulting in the sequence for the mutant strand shown. What was the original amino acid? c. Compare the charges and pl of the mutant peptide and the normal (wild- type) peptide at physiological pH?