A mutated form of the a subunit of the heterotrimeric G protein has been identified. It is found to readily exchange GDP for GTP even the absence of an activated receptor. a. What would be the effect on a signaling pathway containing the mutated protein? And in light of your answer would this protein more likely to function as an oncogene or as a tumor suppressor. Briefly explain your answer. b. A paper finds an interesting study where an analog of GTP (GTPYS, guanosine 5'-0-[gamma-thio]triphosphate) is used to alter G-protein to activity. The authors claim that the data below suggest that it could perhaps be used as a treatment for patients with the mutated a subunit as described above. What do you think? [PJ GTPYS Rate of Hydrolysis Hill GTP III GTPYS Time

Biochemistry
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Chapter1: Biochemistry: An Evolving Science
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**Transcription for Educational Website**

A mutated form of the α subunit of the heterotrimeric G protein has been identified. It is found to readily exchange GDP for GTP even in the absence of an activated receptor.

a. What would be the effect on a signaling pathway containing the mutated protein? And in light of your answer would this protein more likely to function as an oncogene or as a tumor suppressor. Briefly explain your answer.

b. A paper finds an interesting study where an analog of GTP (GTPγS, guanosine 5'-O-[gamma-thio]triphosphate) is used to alter G-protein activity. The authors claim that the data below suggest that it could perhaps be used as a treatment for patients with the mutated α subunit as described above. What do you think?

**Diagram Explanation**

The diagram includes a chemical structure of GTPγS to the upper right. Below the structure is a graph illustrating the "Rate of Hydrolysis" over "Time." The graph features two curves: 

- The top curve represents GTP and shows a higher rate of hydrolysis, with increasing concentration [Pi] over time.
- The bottom curve represents GTPγS, which shows a lower rate of hydrolysis with a relatively stable concentration [Pi] over the same time period.

Error bars on the points indicate variability in the data. This suggests that GTPγS may alter G-protein activity differently than GTP.
Transcribed Image Text:**Transcription for Educational Website** A mutated form of the α subunit of the heterotrimeric G protein has been identified. It is found to readily exchange GDP for GTP even in the absence of an activated receptor. a. What would be the effect on a signaling pathway containing the mutated protein? And in light of your answer would this protein more likely to function as an oncogene or as a tumor suppressor. Briefly explain your answer. b. A paper finds an interesting study where an analog of GTP (GTPγS, guanosine 5'-O-[gamma-thio]triphosphate) is used to alter G-protein activity. The authors claim that the data below suggest that it could perhaps be used as a treatment for patients with the mutated α subunit as described above. What do you think? **Diagram Explanation** The diagram includes a chemical structure of GTPγS to the upper right. Below the structure is a graph illustrating the "Rate of Hydrolysis" over "Time." The graph features two curves: - The top curve represents GTP and shows a higher rate of hydrolysis, with increasing concentration [Pi] over time. - The bottom curve represents GTPγS, which shows a lower rate of hydrolysis with a relatively stable concentration [Pi] over the same time period. Error bars on the points indicate variability in the data. This suggests that GTPγS may alter G-protein activity differently than GTP.
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