In studies trying to understand the regulation of hormone-sensitive adenylate cyclase, it was discovered that non-hydrolyzable GTP analogs and cholera toxin (that inhibits the GTPase activity of Gs ) were able to activate the enzyme in a sustained (irreversible) profile, therefore indicating that GTP hydrolysis represents a deactivation (turn-off) regulatory step. a) Will these analogs or toxin have any effect on adenylate cyclase activity in the absence of any added hormone? b) If you add an factor that increases the rate of GTPase hydrolysis of the Gs protein, will this activate or inhibit the observed downstream effect upon addition of the hormone (this is in the absence of GTP analogs or toxin). Explain your answer.
In studies trying to understand the regulation of hormone-sensitive adenylate cyclase, it was discovered that non-hydrolyzable GTP analogs and cholera toxin (that inhibits the GTPase activity of Gs ) were able to activate the enzyme in a sustained (irreversible) profile, therefore indicating that GTP hydrolysis represents a deactivation (turn-off) regulatory step. a) Will these analogs or toxin have any effect on adenylate cyclase activity in the absence of any added hormone? b) If you add an factor that increases the rate of GTPase hydrolysis of the Gs protein, will this activate or inhibit the observed downstream effect upon addition of the hormone (this is in the absence of GTP analogs or toxin). Explain your answer.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
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In studies trying to understand the regulation of hormone-sensitive adenylate
cyclase, it was discovered that non-hydrolyzable GTP analogs and cholera toxin (that inhibits the
GTPase activity of Gs ) were able to activate the enzyme in a sustained (irreversible) profile, therefore
indicating that GTP hydrolysis represents a deactivation (turn-off) regulatory step.
a) Will these analogs or toxin have any effect on adenylate cyclase activity in the absence
of any added hormone?
b) If you add an factor that increases the rate of GTPase hydrolysis of the Gs protein, will
this activate or inhibit the observed downstream effect upon addition of the hormone (this is in the
absence of GTP analogs or toxin). Explain your answer.
cyclase, it was discovered that non-hydrolyzable GTP analogs and cholera toxin (that inhibits the
GTPase activity of Gs ) were able to activate the enzyme in a sustained (irreversible) profile, therefore
indicating that GTP hydrolysis represents a deactivation (turn-off) regulatory step.
a) Will these analogs or toxin have any effect on adenylate cyclase activity in the absence
of any added hormone?
b) If you add an factor that increases the rate of GTPase hydrolysis of the Gs protein, will
this activate or inhibit the observed downstream effect upon addition of the hormone (this is in the
absence of GTP analogs or toxin). Explain your answer.
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