6. A control phospholipid membrane is isolated in which the phospholipid tails all havean 18-C chain length and are comprised of a 50:50 mixture of saturated andunsaturated tails. In addition, about 25% of the lipids are fluorescently labeled onthe head groups.a) Draw a single plot of Fluidity vs. Temperature that contains the expected curvesfor:Control phospholipid (line- -)Control supplemented with 18-C saturated phospholipids (dashed line -Control supplemented with 16-C chain length (dotted line • • • • • • )b) Draw a single plot of the Fluorescence Intensity vs. Time that contains theexpected curves of a Fluorescence Recovery After Photobleaching (FRAP) usingthe same samples as in (a) above. The FRAP experiment is conducted at thesame temperature for each sample (which is the Tm of the of the controlphospholipid).

Phospholipids are major membrane lipids and present as a bilayer structure. It is consist of…

Answered: 6. A control phospholipid membrane is…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Consider a mixture comprised of the proteins attached: Protein that will most strongly bind to an anion exchange column? Protein that will elute first in gel filtration chromatography? Protein that will elute last in hydrophobic interaction chromatography? Protein that will elute last in carbohydrate containing column?
1. Complete the table below with information about the amino acids utilized in this pro- cedure. Remember that smaller amino acids will travel further and so will amino acids that are soluble in the solvent. Table 9-1. Amino Acids Procedure AMINO ACIDS Phenylalanine Aspartic Acid Leucine Proline matemps DRAW THE MOLECULAR STRUCTURE in POLARITY (IS IT POLAR OR NONPOLAR?) odme her basalu la MOLECULAR WEIGHT SIZE (RANK LARGEST = 4 AND SMALLEST = 1) SHOULD IT REACT WITH NINHYDRIN?
A solution containing two globular proteins each with a single subunit (one is "large" with a radius of 47 Å and the other is "small" with a radius of 16 A) is applied to a gel-filtration (i.e. size-exclusion) column. What is the most likely outcome? O a The outcome will depend on which protein has the most negative surface charge. O b. The small protein elutes before the large protein. O C. The large protein elutes before the small protein. O d. Both proteins elute together. O e. The outcome will depend on the pH of the solution.
It seems paradoxical that a lipid bilayer can be fluid yet asymmetrical. Explain.
How does NH4SO4 affect water structure? What does this have to do with protein solubility? I thought the answer was that NH4S04 attracts H2O so the protein doesn't bind to H2O allowing the protein to bind to each other. But the answer given is, orders water locally to become more ice-like. Can someone explain?
Supported lipid bilayers (SLBs) are widely used in biophysical research to investigate the effect of drugs on lipid membranes and may be prepared by different methods. a. State two methods for the formation of SLBs. b. Describe, with the aid of diagrams, one of the previously stated methods for preparing SLBs. Provide any experimental conditions that may affect or promote the formation of the SLB.
(c) The tube and cylinder diagram to the right illus- trates schematically the potassium channel protein from a bacterium. This channel protein consists of four identical polypeptide chains each comprised of 119 residues. The cyan ribbon illustrates only two of the polypeptide chains. The relative positions of 4 α-helices, one from each poly- peptide chain, are shown as cylinders. The red sphere in the central part of the diagram shows the position of a potas- sium ion identified by X-ray structure analysis. A cesium ion (green sphere) was also found to be stabilized in the same position when cesium chloride was introduced into the crys- tals. The cylinders each represent an α-helix running from Tyr62 to Thr74. Considering the position of the potassium ion, in- dicate on one of the cylinders at which end of the cylinder residue Tyr62 is found and at which end residue Thr74 is found. Explain the basis of your answer? Ala23 Thr119 (d) The N- and C-terminal residues of one of the…
. Two separate synthetic lipid membranes were prepared using palmitic acid and linoleic acid, singly in each. When both the membranes were tested for their transition temperatures, the one made of palmitic acid showed a higher value than the other. Analyse the situation (
2. | Calculate the overall charge (pH 7) on the following three polypeptides and answer the questions below. Assume the following pKa values: N-terminal -NH3®, 7.0; all -COOH groups, 4.0; Arg, 12.5; Cys, 8.4; His, 6.0; Lys, 10.0; Tyr, 10.0. (A) Ser-Tyr-Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Val-Gly-Lys-Lys-Arg-Arg-Pro-Val-Lys-Val-Tyr-Pro-Asp-Ala -Gly- Glu-Asp-Gln- Ser-Ala-Glu-Ala-Phe-Pro-Leu-Arg-Glu-Phe (B) Ser-Tyr-Ser-Met-Glu–His-Phe-Arg–Trp–Gly-Ala-Pro-Val-Gly-Glu-Glu-Cys-Asp-Pro-Val-Glu–Val-Tyr-Pro-Asp- Ala-Gly-Glu-Asp-Gln-Ser-Ala-Glu-Ala-Phe-Pro-Leu-Glu-Phe-Cys-Ser-Tyr-Ser-Met-Glu-His-Phe-Asp-Trp-Gly- Asp-Pro-Val-Gly-Pro-Asp-Ala-Gly-Asp-Gln-Pro-Val-Gly-Glu-Glu-Cys-Asp-Pro-Val-Glu-Val-Tyr-Pro-Asp-Ala | (C) Gly-Ser-Val-Arg-Asp-Pro-Val-Lys-Glu–Val-Tyr-Pro-Asp- Lys-Ala-Gly-Arg-Glu-Ser-Arg-Ala (a) Which of the three peptides would elute first from a gel filtration column? (b) Which of the three peptides would migrate the fastest on SDS-PAGE (c) Which of the three peptides could be…
Describe how you might extract membrane proteins from a mouse cell culture without using a commercial kit. Your proposal should take into account that the membrane protein sample extracted should be compatible with a subsequent purification step involving ion exchange chromatography. Justify your choices of extraction steps and reagents that you might use.
Draw a hydropathy plot and a 2D protein cartoon for a protein that passes through the lipid bilayer 6 times..
4. A protein solution is prepared by dissolving 350 µg of protein in 200 µL of water. A 150 μL sample of this solution is diluted to a total volume of 4.5 mL. How many mg of protein will be in a 2 mL sample of the diluted protein solution? Space to show your workings:

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