Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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6. A control phospholipid membrane is isolated in which the phospholipid tails all have
an 18-C chain length and are comprised of a 50:50 mixture of saturated and
unsaturated tails. In addition, about 25% of the lipids are fluorescently labeled on
the head groups.
a) Draw a single plot of Fluidity vs. Temperature that contains the expected curves
for:
Control phospholipid (line- -)
Control supplemented with 18-C saturated phospholipids (dashed line -
Control supplemented with 16-C chain length (dotted line • • • • • • )
b) Draw a single plot of the Fluorescence Intensity vs. Time that contains the
expected curves of a Fluorescence Recovery After Photobleaching (FRAP) using
the same samples as in (a) above. The FRAP experiment is conducted at the
same temperature for each sample (which is the Tm of the of the control
phospholipid).
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Transcribed Image Text:6. A control phospholipid membrane is isolated in which the phospholipid tails all have an 18-C chain length and are comprised of a 50:50 mixture of saturated and unsaturated tails. In addition, about 25% of the lipids are fluorescently labeled on the head groups. a) Draw a single plot of Fluidity vs. Temperature that contains the expected curves for: Control phospholipid (line- -) Control supplemented with 18-C saturated phospholipids (dashed line - Control supplemented with 16-C chain length (dotted line • • • • • • ) b) Draw a single plot of the Fluorescence Intensity vs. Time that contains the expected curves of a Fluorescence Recovery After Photobleaching (FRAP) using the same samples as in (a) above. The FRAP experiment is conducted at the same temperature for each sample (which is the Tm of the of the control phospholipid).
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