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5. The diagram on the right illustrates experimental results taken from a publication by Peter Mitchell. Intact rat liver mitochondria were sus- pended in an anaerobic, isotonic KCI solution buffered to pH 7.2 with dilute glycylglycine. The pH of the suspension was monitored with a special, fast acting pH electrode, as illustrated on the right. An aliquot of 3-hydroxy-butyrate solution was added to a final concentration of 2 mM together with a pulse of O2. No other reagents were added prior to addition of the uncoupler FCCP indicated by the red arrow. (a) Under these experimental conditions in which mitochon- drial compartment is the proton concentration being monitored. Ex- plain. (b) When 3-hydroxybutyrate has been supplemented to the mitochondrial suspension, is the rate of respiration supported by substrate oxidation maximal or resting prior to addition of the un- coupler? Explain. (c) addition of the uncoupler? Explain. Does the pH of the medium increase or decrease upon 0/+H+ 10 Port for reagent addition 1 12 14 16 18 Time (sec.) pH sensitive surface suspension of mitochondria in 0.15 M KCI kept under gentle stirring. Mitochondrion, not in scale to electrode (d) Name the relevant enzymes, and, using structural formulas, write the two chemical reactions underlying the oxidation of 3-hydroxybutyrate that serves as the source of electrons fed into the electron transfer chain in this experiment.

5. The diagram on the right illustrates experimental results taken from a publication by Peter Mitchell. Intact rat liver mitochondria were sus- pended in an anaerobic, isotonic KCI solution buffered to pH 7.2 with dilute glycylglycine. The pH of the suspension was monitored with a special, fast acting pH electrode, as illustrated on the right. An aliquot of 3-hydroxy-butyrate solution was added to a final concentration of 2 mM together with a pulse of O2. No other reagents were added prior to addition of the uncoupler FCCP indicated by the red arrow. (a) Under these experimental conditions in which mitochon- drial compartment is the proton concentration being monitored. Ex- plain. (b) When 3-hydroxybutyrate has been supplemented to the mitochondrial suspension, is the rate of respiration supported by substrate oxidation maximal or resting prior to addition of the un- coupler? Explain. (c) addition of the uncoupler? Explain. Does the pH of the medium increase or decrease upon 0/+H+ 10 Port for reagent addition 1 12 14 16 18 Time (sec.) pH sensitive surface suspension of mitochondria in 0.15 M KCI kept under gentle stirring. Mitochondrion, not in scale to electrode (d) Name the relevant enzymes, and, using structural formulas, write the two chemical reactions underlying the oxidation of 3-hydroxybutyrate that serves as the source of electrons fed into the electron transfer chain in this experiment.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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5. The diagram on the right illustrates experimental results taken from a publication by Peter Mitchell. Intact rat liver mitochondria were sus- pended in an anaerobic, isotonic KCI solution buffered to pH 7.2 with dilute glycylglycine. The pH of the suspension was monitored with a special, fast acting pH electrode, as illustrated on the right. An aliquot of 3-hydroxy-butyrate solution was added to a final concentration of 2 mM together with a pulse of O2. No other reagents were added prior to addition of the uncoupler FCCP indicated by the red arrow. (a) Under these experimental conditions in which mitochon- drial compartment is the proton concentration being monitored. Ex- plain. (b) When 3-hydroxybutyrate has been supplemented to the mitochondrial suspension, is the rate of respiration supported by substrate oxidation maximal or resting prior to addition of the un- coupler? Explain. (c) Does the pH of the medium increase or decrease upon addition of the uncoupler? Explain. Mitochondrion, not in scale to electrode (d) Name the relevant enzymes, and, using structural formulas, write the two chemical reactions underlying the oxidation of 3-hydroxybutyrate that serves as the source of electrons fed into the electron transfer chain in this experiment. →H*/0 10 Port for reagent addition 12 14 16 18 Time (sec.) 20 22 pH sensitive surface suspension of mitochondria in 0.15 M KCI kept under gentle stirring.
Transcribed Image Text:5. The diagram on the right illustrates experimental results taken from a publication by Peter Mitchell. Intact rat liver mitochondria were sus- pended in an anaerobic, isotonic KCI solution buffered to pH 7.2 with dilute glycylglycine. The pH of the suspension was monitored with a special, fast acting pH electrode, as illustrated on the right. An aliquot of 3-hydroxy-butyrate solution was added to a final concentration of 2 mM together with a pulse of O2. No other reagents were added prior to addition of the uncoupler FCCP indicated by the red arrow. (a) Under these experimental conditions in which mitochon- drial compartment is the proton concentration being monitored. Ex- plain. (b) When 3-hydroxybutyrate has been supplemented to the mitochondrial suspension, is the rate of respiration supported by substrate oxidation maximal or resting prior to addition of the un- coupler? Explain. (c) Does the pH of the medium increase or decrease upon addition of the uncoupler? Explain. Mitochondrion, not in scale to electrode (d) Name the relevant enzymes, and, using structural formulas, write the two chemical reactions underlying the oxidation of 3-hydroxybutyrate that serves as the source of electrons fed into the electron transfer chain in this experiment. →H*/0 10 Port for reagent addition 12 14 16 18 Time (sec.) 20 22 pH sensitive surface suspension of mitochondria in 0.15 M KCI kept under gentle stirring.
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