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Identify five (5) errors in the figure below. Draw a corrected version below it and encircle all the corrections that you made.
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- A health provider contacts you regarding a specific patient. They ask you to sequence this patient’s DNA and sends you her blood sample. You split the patient’s blood sample into three equal parts. With each part, you perform three different protocols of DNA extraction and purification. You analyze the resulting DNA using the nanodrop. Protocol A260/280 ng/ul DNA Total volume (ul) 1 2.02 50 60 2 1.45 156 200 3 0.30 300 120 d)Halfway through the electrophoresis of your gel you realized that rather than using a buffer to make up your gel, you actually used distilled water. Do you think this mistake would alter the outcome of your gel electrophoresis? Explain. e) If you added your DNA to a well in a gel without first mixing it with gel loading solution, would this impact the final results? Explain. f) Only after the run you realize that you forgot to add ethidium bromide to the gel. What will happen to your sample? What could you do to the gel after the run?Please help me to write the complementary strands of the following: 1. A strand of DNA has the base sequence ATGATC. Write the base sequence for the complementary strand. 2. A strand of DNA has the base sequence GATTCA. Write the base sequence for the complementary strand. Thank you very much for your help.Consider the four extraction/purification methods . a. Which extraction method would you use for a touch DNA sample? Explain your reasoning.b. Which extraction method would you use for a sample containing a mixture of primarily non-human and some human DNA? INFO: the 4 extraction methods are QIAamp manual extraction, QIAcube semi-automated extraction, DNA IQ extraction, or FTA punch purification
- Listed below are 4 of the 13 genome sites used to create a standard DNA profile. Each site consists of a number of short tandem repeats: sets of 4 nucleotides repeated in a row within the genome. For each site, the number of repeats found at that site for this individual are listed: Imagine you perform a PCR procedure to create a DNA profile for this individual. Which of the following four gels correctly represents the DNA profile of this person?I need help with the following question I dont understand a) and b)!!!!! Dr. Sharon Martin and her company have spent years working to identify how the gene for albinism works. The mutation in this gene causes no pigment to be produced in the hair, skin or eyes. Identifying the gene would open the door to curing the condition. Finally, her team succeeds. But, the years of research have been expensive. One way to make back the money is to patent the gene that the team members have identified. Then, anyone who wanted to develop either treatments or tests would have to pay a fee to use the gene. a) Do you think the government should allow this gene to be patented? Explain. b) If Dr. Martin’s company develops a test for this gene, should they be allowed to patent the test? Explain.The Evidence shows which suspect was at the crime scene? ( image of murder investigation) A) suspect A B) suspect B C) suspect C D) none were at the crime scene Which of the following choices most likely lists the sizes of the four DNA fragments in lane 6 ( second image) of this agarose gel stained with ethidium bromide? ( Note: the well that the DNA was loaded into is at the top of the gel image). A) 100 bp, 300 bp, 450 bp, 600 bp. B) 300 bp, 400 bp, 100 bp, 600 bp C) 600 bp, 450 bp, 300 bp, 100 bp D) 400 bp, 300 bp, 600 bp, 100 bp E) DNA fragment size can't be estimated using an agarose gel.
- DNA EXTRACTION Materials: knife 1 cup of fruit Table salt clean piece of cloth or strainer for filtering resealable bag dishwashing liquid 70% rubbing alcohol (chilled) shot glass (or any transparent and narrow container resembling a test tube) How to do the extraction: Before you begin, make sure you have chilled your alcohol in the freezer for at least 3 hours. Room temperature alcohol will not work nearly as well as cold alcohol. Place about 50 mL of alcohol in the freezer to chill it and take it out only when you’re going to need it. (The alcohol will not actually freeze.) 1. The first thing you will need is a sample. Since DNA is found in all living…Please answer the following questions using numbers and not text. For example, if you think the answer is 24, write 24 and not twenty-four. If you think the answer is 24 ATP, write only the number 24. As a microbiology technician, your job is to characterize a novel bacterial strain isolated from insects. Phenotypically, you have discovered that the new bacterial strain can grow in the presence or absence of oxygen. After genome sequencing and biochemical characterization, you know that your new organism is capable of making all of the enzymes necessary for glycolysis and the TCA cycle, and that it has an electron transport chain. Answer the following questions based on the growth of your organism on minimal media plates containing only acetate as a carbon and energy source. Acetate is transported across the membrane at the cost of 3 ATP per molecule. Each question should be answered based on the metabolism of one molecule of acetate. If you come up with a negative value, indicate an…Given the electrophoresis profile of a Sanger sequencing result, what was the sequence of the original DNA sample used for sequencing? ddATP ddGTP ddCTP ddTTP - GGTTACC B CCATTGG CCAATGG GGTAACC | | | |
- What is the purpose of the following substances in the dna extraction procedure: Detergent Pineapple juice/Contact lens solution SaltThe following statements are either accurate as written or contain some errors. You need to rewrite each as an accurate statement. Make sure that you do not change the material the sentence is about. Highlight or underline any changes that you make to statements. Thank you! 1. Primers cleave DNA at recognition sequences. They can produce either matching ends, which are fragments with overhangs, or it can produce blunt ends, which are fragments without overhanging ends. 2. Codons are three nonoverlapping (such that each ribonucleotide is only in one codon) ribonucleotides that specify a particular amino acid. They are "read" in a linear fashion by the ribosome to be translated into a polypeptide. 3. The diagram below is an illustration of an important molecule for transcription. It depicts a ribosome that has two important regions. Region A that attaches to an amino acid and Region B that contains a codon.Given the electrophoresis profile of a Sanger sequencing result, what was the sequence of the original DNA sample used for sequencing? GGTAACC CCAATGG GGTTACC CCATTGG