2. *REQUIRED 1 A somatic cell in an organism develops a DNA mutation. This mutation is a beneficial mutation that causes the production of a more efficient enzyme for digestion. Where will the mutation be found over time? In the organism's immediate offspring only In the organism only In the organism, its offspring, and all of its direct descendants In the organism and its immediate offspring only

216 Inquiry into Life Laboratory Manual, 16th Edition ©2020 Mader Experimental Procedure: Wing Length and Body Color in Drosophila If your instructor has frozen flies available, cross out the numbers in Table 22.4 and use the stereomicroscope or a hand lens to count the flies of each type given in Table 22.4. Otherwise simply use the data supplied for you in Table 22.4. Table 22.4 Wing Length and Body Color in Drosophila* Phenotypes Long Gray Long Ebony Short Short Phenotypic Gray Ebony Ratio Number of offspring 28 32 28 30 Class data 128 120 120 120 *Wings and body are understood in this table. Conclusions: Wing Length and Body Color in Drosophila • Calculate the actual phenotypic ratio based on the data and record in Table 22.4. Do the results differ from the expected ratio per individual data?. Per class data? Explain your answer. Two-Trait Genetics Problems 1. In tomatoes, tall is dominant and short is recessive. Red fruit is dominant and yellow fruit is recessive. Choose a key for…

. While perusing the E. coli K12 genome sequence,you come across a gene with no known function. Theamino acid sequence of the gene’s protein productshows weak similarities with known porins, proteinsthat cross a cellular membrane to let molecules suchas amino acid or sugar nutrients (or drugs like penicillin) pass through. Some porins are nonspecific and letany solute up to a certain size transit into the cell.Other porins are specific and allow the transit ofcertain sugars but not others. What genetic experiments could you do to try to determine whether thisnew gene has a specific function in allowing bacterialcells to scavenge the sugar maltose from the environment? Describe scenarios that might complicate yourexperimental approach.

14. DNA methyltransferase, an enzyme that methylates DNA, - ---- a. is expressed in a higher than normal amount in a tumor cell; its location is nuclear b.. is expressed in a higher than normal amount in a tumor cell; its location is cytoplasmic c. is expressed in a lower than normal amount in a tumor cell; its location is nuclear d. . is expressed in a lower than normal amount in a tumor cell; its location is cytoplasmic 15. In human cancers, a. Rb promoter is hypermethylated b. expression of CDK inhibitors is inhibited c. there is high level of cyclin D-CDK4/6 kinase activity, phosphorylating pRb d. a and b e. a, b and c 16. Cancer causing agents bring about their effect mainly in G1 phase of the cell cycle because a. the cell has just emerged from the quiescent G0 phase b. Gl phase is the longest c. All enzymes and cyclins are active in the G1 phase d. Cells respond to their extracellular growth signals during the Gl phase

Human Biology- MILESTONE 6 X P genetics-and-biotechnology-glo O https://app.sophia.org/spcc/human-biology-2-mile -> Sophia Pathways M Gmail Purdue Global - Sig... Welcome - Login.gov UNIT 6 – MILESTONE 6 Which of the following is a gene abnormality in which two nonhomologous parts rearrange and fuse together? O Translocation O Deletion None of these, O Duplication SAVE & CONTINUE Report an issue with this question Type here to search

10. Relative to one of your liver cells, one of your skin cells has, the same function the same genome and a different pattern of gene expression a different pattern of gene expression a different pattern of gene expression and the same function the same genome (i.e., the same genetic material)

True or False

1. Determine the effect of the following mutations on the DNA sequence. In each case, the mutation is described after the sequence (REFER TO THE SUPPLEMENTAL DOCUMENT FOR GUIDANCE TO THIS QUESTION). Adenine nucleotide (A shown in red below) was inserted into the DNA sequence at the position indicated by the arrow). Write out the sequence of the mutated DNA and the protein made from it. What is the effect of this mutation on the protein? (For example, how will the mutation affect the length and sequence of the protein? What about the function of the protein?) A 3' TACATGG'TTGTGCTAATT 5'

1. You are interested in the eukaryotic protein/enzyme Thiolase, which is 200 amino acids in length. Because Thiolase is only active in the peroxisome (an organelle) you know there must be a peroxisomal localization signal sequence in the protein. Sub-cellular Location Construct of GFP protein In order to be able to visualize the Thiolase protein in a cell, you create a fusion Cytosol construct with the gene for Thiolase and the GFP gene for Green Fluorescent Protein (GFP). 200 Thiolase Peroxisome GFP You also make a series of deletions in the 50 200 Peroxisome gene for Thiolase, all fused with the GFP GFP 100 200 gene. Numbers on top of the constructs GFP Peroxisome represent amino acid positions. 125 200 Cytosol GFP You express each construct in a mammalian 150 200 cell and use a microscope to visualize where the Thiolase-GFP protein gets trafficked. cytosol GEP 150 Peroxisome GFP Using the data provided, where must the peroxisomal localization signal sequence be in the Thiolase…

5a. In Activity 1, you may have noticed that we mutagenized a haploid strain. Haploid strains are easier than diploid strains when screening for recessive loss-of-function mutations. Why?