2. Compare and Contrast the horizontal gene transfer mechanisms of Transformation, Generalized Transduction, and HFR Conjugation. Identify the basic steps of each process and identify each mechanism's unique features. A. Transformation: 1. Basic process 2. Unique features: B. Generalized Transduction: 1. Basic process 2. Unique features: C. HFR Conjugation: 1. Basic process es

2. Compare and Contrast the horizontal gene transfer mechanisms of Transformation, Generalized Transduction, and HFR Conjugation. Identify the basic steps of each process and identify each mechanism's unique features. A. Transformation: 1. Basic process 2. Unique features: B. Generalized Transduction: 1. Basic process 2. Unique features: C. HFR Conjugation: 1. Basic process es

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 4TYK

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Which of the following is not correct for bacterial conjugation? a. Both Hfr and F+ bacteria have the ability to code for a sex pilus. b. After an F- cell has conjugated with an F+, its plasmid holds the F+ factor. c. The recipient cell is Hfr following conjugation. d. In an HfrF- conjugation, DNA of the main chromosomemoves to a recipient cell. e. Genes on the F factor encode proteins of the sex pilus.
Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning vectors? a. The plasmid must have resistance to accept DNA inserts. b. They allow the detection of plasmids that contain an inserted DNA fragment. c. They ensure the presence of the ori site. d. They ensure that the plasmid can be cut by a restriction enzyme. e. They allow identification of bacteria that have taken up a plasmid.
7. Why recombinant DNA is very useful in improving our health conditions?A. Human insulin can be reproduced by bacteria?B. Vaccine can be reproduced by fungi or bacteriaC. Human growth hormone can be obtained from E. ColiD. All of the above 8. Which of the following is/are example of genetic engineering?A. A farmer choose a breed of cow for greater milk production.B. The use of bacteria in order to produce human insulinC. The insertion of clone genes to plant cellsD. Cross pollination of squash flowers. 9. Bacterial cells as host organisms may expose the gene to make protein products such as insulin and growth hormone . Which of the following processes are involved?A. replication and transcription. B. Transcription and replication C. Transcription and transformation. D. Replication and transformation
1- A genetic analysis of a bacterium reveals the presence of viral DNA. The most probable way this happened was through (a)mutation (b) conjugation (c)transduction (d)transposons (e)transformation 2- Which statemere is tobe regarding GEMC (a)typical cloning vectors involbe plasmids and viruses (b)eukaryotic genes may only be introduced and expressed in eukaryotic microbes such as yeasts (c)horizontal gene transfer methods may be manipulated to introduce new genes (d) expressions of introduced genes can be monitored through the use of marker genes. (e)it is possible for multiple genes may be added to microbes from other sources . plz give answer for both questions asap please
1. Write if the statement is true and modify the statement with the correct answer if its false a.White colonies represent those bacteria that do not express the genes for beta-galactosidase because restriction enzymes have cut the gene and ligase have not repaired the break b.Antibiotic resistance genes in bacterial transformation are used as marker genes because of ease of detection of transformants. c. Bacterial transformation simply means the insertion of a recombinant plasmid into a bacterial cell.
2- Which statement is false is regarding GEMC ? (a)typical cloning vectors involbe plasmids and viruses (b)eukaryotic genes may only be introduced and expressed in eukaryotic microbes such as yeasts (c)horizontal gene transfer methods may be manipulated to introduce new genes (d) expressions of introduced genes can be monitored through the use of marker genes. (e)it is possible for multiple genes may be added to microbes from other sources . asap please
1. Through observing the sequence of events (A → B → C → D) found below, describe how reproductive cloning is made possible. Your response should consist of 3—5 complete sentences. SENTENCE STARTER: The process of cloning may look quite tricky, but it can actually be broken down into 4 individual steps. In step A, the cells of the organism we want to be cloned are isolated and the… 2. What is an ADVANTAGE of engaging in cloning practices similar to the one highlighted above? What reason might a farmer, livestock owner, or someone else use as justification for cloning? 3. What is a DISADVANTAGE (or potential drawback) of cloning plants, livestock, or pets?
1. What are the considerations we need to consider in introducing recombinant DNA in plant calli and in animal cell lines? (Give at least three considerations) Explain and answer this comprehensively.
1. Write if the statement is true and modify the statement with the correct answer if its false a.Blue colonies in the white-blue screening provide sufficient information on the transfer of recombinant DNA in bacterial cells. b.In blue-white screening, it is only the blue colonies that have survived the effect of antibiotics in the media. c.Bacterial transformation simply means the insertion of a recombinant plasmid into a bacterial cell. d.Competent cells are those bacterial cells that can accept the entry or re-entry of plasmids.
2. Discuss the use of transposons as mutagens in bacteria.
9. Identify one specific example of how this process has been used 10. Using the example provided in question 9, explain the rationale for the creation of this transgenic organism
1. It is a double-stranded , self replicating, circular DNA molecule present in bacteria which is widely used as a gene cloning vector.A. Cosmid B. Plasmid. C. Phagemid. D. Genome 2. In plant genetic engineering, which of the following acts as vector?A. Agrobacterium lumefaciens. B. Gene of interest C Receipt plant cell. D. Ti-plasmid 3. Which of the following illustrates breeding?A. A farmer choose a breed of cow for greater milk productionB. The use of bacteria in order to produce human insulin.C. The insertion of cloned genes to plant cells.D. All of the above. 4. Being pest resistance is one of the traits that are being introduced to plants like corn and eggplant with the insertion of Bt-toxin gene to plant cell. What method is use when a gene is inserted to plants?A. Biolistic B. Electroporation C. Plasmid insertion by heat shock treatment D. All of these 5. A biotechnologist wants to enhance the size of duhat fruit because research shows that it can lower blood sugar but is very…