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- True or false? SDS PAGE gel can be prebed with NBT/BCIP to perform an immunoblot. The direction of proteins through a matrix is due to most proteins in nature having a net negative charge. PBST is buffer that is used for blocking the membrane during immunoloblotting.Hybridoma technology allows one to generatemonoclonal antibodies to virtually any protein. Why isit, then, that genetically tagging proteins with epitopes issuch a commonly used technique, especially since an epi-tope tag has the potential to interfere with the function ofthe protein?pathogenic bacteria in gut lumen binds binds capture and transfer to A B submucosa inhibits binds produces produces epithelial cells of small intestine klocated below B-cll follicle in Peyer's patches reside on reside presents among antigens E F capture and transfer from lumen presents engulf antigens pathogenic bacteria in gut submucosa D -lyse-
- The head of the oncology divison of a large biotech company proposes to develop a therapeutic antibody to treat cancers with mutant B-Raf oncogene. Is this a good idea? Why or why not(limit 3-4 sentences)?n western blotting, secondary antibody is conjugated with color or fluorescence producing enzyme systems to generate signal. True FalseWhich statement is right the polyclonal antibody is expensive to produce the monoclonal antibody has relatively high specificity and sensitivity in western blot analysis C. monoclonal antibody rather than polyclone antibody can be used for therapy only hybridoma technology can produce monoclonal antibodies E. polyclonal antibodies are always welcome in molecular detection
- Labs that do a lot of Western blots often have hundreds of primary bodies but only two or three secondary antibodies. Why? I understand that secondary antibody aids in the detection, sorting or purification of target antigens by binding to the primary antibody which directly binds to the target antigen. However, I don't undrstand why there are only two or three secondary antibodies available in the laboratory. Glad if the expert would advise.Why do we use secondary antibodies in Western Blotting? Intensify signal from 1° antibody O Bind directly to an antigen O No answer text provided. O Remove unbound 1° antibodyRheumatoid Factor RF Latex Test RF is a rapid latex agglutination test kit for the detection of rheumatoid factor (RF) in human serum. RF is found in sera of patients with rheumatoid arthritis and is believed to be IgM antibodies directed against the patients own immunoglobulin G. Principle: The RF latex particles are coated with specially purified human gamma globulin. When the latex suspension is mixed with serum containing elevated RF levels on a slide, clear agglutination is seen within 2 minutes. Questions 1. Is Rheumatoid Arthritis an autoimmune disease? 2. Differentiate the different types of Immunoglobulins 3. Differentiate Rheumatoid Arthritis from Gouty Arthritis
- What is the major difference in antigen-processing between major histocompatibility complex class-I (MHC-I) and MHC-Il immune signaling? Edit View Insert Format Tools Table 12pt v Paragraph v BIUAYou are interested in performing indirect immunofluorescence light microscopy to observe the localization of the catalase enzyme in the cultured HeLa cells, obtained historically from the cervical tumor of Henrietta Lacks. You were going through the lab stock and found a few primary and secondary antibodies. Which of the following secondary antibody can you use in your experiments? O All of the mentioned antibodies can be used in the experiment Goat anti-human antibody conjugated to 10 nm gold Goat-anti-human catalase conjugated to 10 nm gold O Human anti-catalase antibody conjugated to fluorescent rhodamine Goat anti-human antibody conjugated to fluorescent rhodamineTrue or false? SDS PAGE gels can be probed with antibidies to perform an immuboblot. C.elegans is a free-living nematode. primary antibodies are always generated in rabbits.