Imagine you are conducting an Ames test experiment. Based off your results (shown in pictures), answer these questions: 1. Generate a fully labeled graph/figure depicting the data generated in lab (minimally you should include the data from control, 2-NF, and two different test compounds.) 2. Make a bulleted list of 3 distinct and specific observations/patterns seen in your graph/figure - for each observation, indicate specifically where you should look to find the observation on your figure.

Essentials Health Info Management Principles/Practices
4th Edition
ISBN:9780357191651
Author:Bowie
Publisher:Bowie
Chapter7: Numbering & Filing Systems And Record Storage & Circulation
Section7.2: Filing Systems
Problem 2E
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Imagine you are conducting an Ames test experiment. Based off your results (shown in pictures), answer these questions: 1. Generate a fully labeled graph/figure depicting the data generated in lab (minimally you should include the data from control, 2-NF, and two different test compounds.) 2. Make a bulleted list of 3 distinct and specific observations/patterns seen in your graph/figure - for each observation, indicate specifically where you should look to find the observation on your figure.
Test Material
No bacteria
2-NF (300 ug/mL) 21/3
16/8
Splenda* (100
mg/mL)
Pickle Juice
Windex
Equal*
Grill surface
Negative control
(P/Y)
16/8
Evape liquid
DMSO Extracted
8/16
18/6
16/8
18/6
(DMSO extracted
from kimwiped
surface)
White Sugar*
10/14
Rust (flaked off of 24/0
a nail)
(DMSO extracted)
Listerine
15/9
20/4
Tobacco from a 23/1
cigarette
(DMSO extract)
*= assume equal molarity
1:10 Test (P/Y) 1:2 Test (P/Y) Test - no dilution (P/Y)
21/3
19/5
0/24
8/16
16/8
20/4
12/12
23/1
24/0
24/0
17/6
23/1
22/2
17/7
16/8
10/14
13/11
13/11
8/16
20/4
11/13
24/0
19/5
10/14
21/3
9/15
12/12
2/22
11/13
12/12
2/22
21/3
13/11
24/0
24/0
7/18
Transcribed Image Text:Test Material No bacteria 2-NF (300 ug/mL) 21/3 16/8 Splenda* (100 mg/mL) Pickle Juice Windex Equal* Grill surface Negative control (P/Y) 16/8 Evape liquid DMSO Extracted 8/16 18/6 16/8 18/6 (DMSO extracted from kimwiped surface) White Sugar* 10/14 Rust (flaked off of 24/0 a nail) (DMSO extracted) Listerine 15/9 20/4 Tobacco from a 23/1 cigarette (DMSO extract) *= assume equal molarity 1:10 Test (P/Y) 1:2 Test (P/Y) Test - no dilution (P/Y) 21/3 19/5 0/24 8/16 16/8 20/4 12/12 23/1 24/0 24/0 17/6 23/1 22/2 17/7 16/8 10/14 13/11 13/11 8/16 20/4 11/13 24/0 19/5 10/14 21/3 9/15 12/12 2/22 11/13 12/12 2/22 21/3 13/11 24/0 24/0 7/18
Control
0
1
2
3
4
Sample
Positive Roots
0.01
0.05
3
5
7
8
DH2345
10
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19 23
20
21
22
23
24
11
12
13
14
15
16
17
18
19
20
21
21
222124
23
5
8
9
11
12
13
15
16
17
18
19
19
20
21
22
22
23
24
24
24
0.001
9
11
12
14
15
16
17
18
19
20
21
22
22
23
24
24
Use these numbers to evaluate mutagenicity in 24-well
plate Ames test format. The numbers listed are the
number of vellow wells in an given quadrant. The
leftmost numbers are the number of yellow wells you
see in your negative control (i.e., no mutagen added)
quadrant.
The other numbers are the number of vellow wells vou
would need to see in your sample wells if your added
chemical was mutagenic. For example, if you see 2
yellow wells in your negative control quadrant, you
would need to see 7 or more yellow wells in a test
quadrant to convince yourself (with a p-value of less
than 0.05) that mutation rate was above background in
your test sample.
Transcribed Image Text:Control 0 1 2 3 4 Sample Positive Roots 0.01 0.05 3 5 7 8 DH2345 10 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 23 20 21 22 23 24 11 12 13 14 15 16 17 18 19 20 21 21 222124 23 5 8 9 11 12 13 15 16 17 18 19 19 20 21 22 22 23 24 24 24 0.001 9 11 12 14 15 16 17 18 19 20 21 22 22 23 24 24 Use these numbers to evaluate mutagenicity in 24-well plate Ames test format. The numbers listed are the number of vellow wells in an given quadrant. The leftmost numbers are the number of yellow wells you see in your negative control (i.e., no mutagen added) quadrant. The other numbers are the number of vellow wells vou would need to see in your sample wells if your added chemical was mutagenic. For example, if you see 2 yellow wells in your negative control quadrant, you would need to see 7 or more yellow wells in a test quadrant to convince yourself (with a p-value of less than 0.05) that mutation rate was above background in your test sample.
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