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- The statement DNA replicates by a semiconservative mechanism means that (a) only one DNA strand is copied (b) first one DNA strand is copied and then the other strand is copied (c) the two strands of a double helix have identical base sequences (d) some portions of a single DNA strand are old and other portions are newly synthesized (e) each double helix consists of one old and one newly synthesized strandThe mismatch DNA repair mechanism is based on the fact that: O a. O b. O C. O d. The parental DNA strand is recognized because it is structurally distorted. The parental DNA strand is methylated while daughter strand is not. The daughter DNA strand is recognized because it is structurally distorted. The daughter DNA strand is methylated while parental strand is not.10. What type of DNA damage would you expect from UV-B irradiation (at 260 nm) of DNA that includes the sequence GCGATGCTTAGA, and how would it be detected and repaired?
- 21. highly methylated DNA is active while less methylated DNA is inactive true or falseReciprocal strand exchange is associated with ... O DNA photolyase site-specific recombination general recombination O DNA excision/repair1. As DNA engages in semi-conservative replication, explain what the following enzymes roles are in this process. ✓✓✓✓ DNA Polymerase II: DNA Helicase: DNA Ligase: Primase:
- a. Pfu Polymerase b.dNTPs c.Buffer Match each component above to the correct function(s) listed below. Write your selection(s) for each component. You may have more than one answer for each. 1. unwinds DNA 2. synthesizes new DNA strands 3. enzymatically catalyzes Quikchange 4. nucleotide source for new DNA strands 5. Energy source for reaction(s) 6. Repairs errors in base pair matching 7. Maintains pH and salt levels 8. Creates polymer chains1. A. Fill in the complementary bases for the blank DNA strand given below: ATGAAGG ATAAAA GAACAGTGG CGTGA1) What is Dna mutaion and its types
- 10. V(D)J recombination contributes to the diversity of immunoglobulins, and occurs by:A. Random single-strand nicks in the DNAB. Programmed double-strand breaks in the DNAC. Site-specific recombinationD. Homologous recombinationE. Retrotransposition 11. Zsuzanna Izsvak and coworkers set out to make a functional copy of a nonfunctional transposase in a fish that is estimated to have been nonfunctional for over 14 million years. The functional transposase became known as ____________________.A. Sleeping BeautyB. RumpelstiltskinC. PandoraD. Rip van WinkleE. None of the above 12. Which of the following Tn3 proteins catalyzes recombination to separate the donor and acceptor DNA molecules?A. transposaseB. DNA polymeraseC. RecAD. ResolvaseE. β-lactamase6. The following diagram shows a replication bubble within a double stranded DNA molecule. The red arrow shows the direction of the primer. AAAGIACGTIGAC ATTGGCC 3' TAACCGG 5' 5' GTCCGAC 3' CAGGCAG C. 5 CAT&CAACTO a. What is the sequence of the red RNA primer? b. If the replication fork moves to the left (left side opens more), will this primer be used to create the leading strand or lagging strand? Please explain your answer. If you answered the leading strand, explain why replication is continuous. However, if you answered lagging strand explain why replication is discontinuous.3c. there are three enzymes that go by the name of DNA polymerase 1 2 and 3. please describe the main functions of each in a cell