Boll weevil is a serious pest of cotton crop.
Effective control involves applications of chemical insecticides,
increasing the cost of production and environmental pollution. The current genetically modified Bt crops have allowed great
benefits to farmers but show activity limited to lepidopteran pests. This work reports on procedures adopted for integration and expression of a cry transgene conferring resistance to boll weevil and fall armyworm by using molecular tools.
Four Brazilian cotton cultivars were microinjected with a minimal linear cassette generating 1248 putative lines.
Complete gene integration was found in only one line (TO-34) containing one copy of crylla detected by Southern blot. Protein was expressed in high concentration at 45 davs after emergence (dae), decreasing by approximately 50% at 90 dae. Toxicity of the cry protein was demonstrated in feeding bioassays revealing 56.7% mortality to boll weevil fed buds and 88.1% mortality to fall armyworm fed leaves. A binding of crylla antibody was found in the midgut of boll weevils fed on T0-34 buds in an immunodetection assay.
The gene introduced into plants confers resistance to boll weevil and fall armyworm.
Transmission of the transgene occurred normally to TI progeny. All plants showed phenotypically normal growth, with fertile flowers and abundant seeds.
Using this information annotate the diagram.
Transcribed Image Text:5F
CaMV35S CaMV35S
EcoRI
BamHI
1F
0.44Kb
3R
3.1 Kb
0.96Kb
13F
crylla
2.1 Kb
0.52Kb
Figure 1. Diagramatic scheme of the construction used for microinjection into cotton bolls via ovary drip.
2R
nos
Sall Hind!!!
Definition Definition Observable physical traits or characteristics of an organism, which are determined by its genetic makeup (genotype) as well as environmental factors. These traits can include anything from eye color or height to behavior or disease susceptibility. Understanding an organism's phenotype is important in fields such as genetics, medicine, and ecology.
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