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GQ12
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- 8. Explain the role of post-translational modifications of protein molecules: the formation of disulfide bridges, hydrolysis of peptide bonds under the action of specific proteases, glycosylation, phosphorylation / dephosphorylation, hydroxylation, acylation of the terminal amino transformation of the terminal carboxyl group into an amide one group and6. Please describe the events that may result in a mature protein not having methionine asthe N-terminal amino acid.9. Which of the following mutations would be least detrimental to the function of a protein and why? 1) Silent; 2) Frameshift; 3) Deletion of two nucleotides; 4) Nonsense; 5) Missense. dreenn ет
- 9. H. State if/how Aureliano's mutation changes the amino acid sequence and describe the effect that change has, if any, on the protein.4. D. State if/how Ursula's mutation changes the amino acid sequence and describe the effect that change has, if any, on the protein.Please ASAP. Thank you. How does the mutation change/affect the structure of the Hb heterotetramer (ie how is quaternary protein structure affected)?
- 18. Chaperones are frequently associated with polypeptides as they are being synthesized from the amnio to carboxy terminus. Provide TWO reasons why it would be advantageous for chaperones to associate with a protein while it is being made?3. List the amino acid sequence of the protein coded for. CCUGCAGUAUGAAACGCCUGGUAGAAGGUGGGAAGUGGUGCGCCC . . .1. Considering the following nucleotide sequence in an mRNA molecule: 5’ AUG UUA CGU AAU GCU GUC GAA UCU AUU UGC UUU ACA UAA 3' d) Write the amino acid sequence of the peptide synthesized from the given mRNA nucleotide sequence. e) Draw the structure of the pertide fragment made up of the first five (5) amino acids in the given polypeptide.
- 1. Determine the effect of the following mutations on the DNA sequence. In each case, the mutation is described after the sequence (REFER TO THE SUPPLEMENTAL DOCUMENT FOR GUIDANCE TO THIS QUESTION). Adenine nucleotide (A shown in red below) was inserted into the DNA sequence at the position indicated by the arrow). Write out the sequence of the mutated DNA and the protein made from it. What is the effect of this mutation on the protein? (For example, how will the mutation affect the length and sequence of the protein? What about the function of the protein?) A 3' TACATGG'TTGTGCTAATT 5'1. Use the info of this molecule as well as the attached addendum to demonstrate the flow of genetic information to protein sequence as described by the so-called “Central Dogma” . Clearly indicate the direction of your polynucleotide strands and peptide/protein. ATG GCA TGC AAT AGC TCA TGC 2. What would happen to the amino acid sequence if the underlined nucleotide (C) would change to an A?1. Show the Dehydration Synthesis reaction that occurs to form a dipeptide containing the following amino acids: i) cysteine and serine leucine and lysine