C. Two mutations are known in the population, one shaded in yellow and one in blue, in the TEMPLATE DNA sequence above. Ursula has a mutation in the yellow-shaded underlined nucleotide: instead of an A in this position, she has a T. 5' AATCA TAACTCA TTG 3' What type of mutation does Ursula have? Missense Silent Nonsense Frameshift

A. Describe the importance of nucleotide metabolism for cells. B. Explain the main features of de novo pathway and salvage pathway of nucleotidemetabolism

2a. The DNA is mutated on the 4th base pair to the following: DNA:3'TAC GAT GAG GTC TGA ACT 5 5' ATG CTA CTC CAG ACT TGA 3 2b. Does this mutation change the amino acid sequence? (Yes or No) If yes – what is new amino acid sequence? Protein:

Q. Deletion of a single AT base pair from codon number 4 can cause a frameshift mutation in a protein-coding gene. Which of the following additional mutations will restore the reading frame back to “normal” such that the original stop codon will still function? (Note that the amino acid sequence will not necessarily be restored back to normal). Adding a base pair into each of the next two codons. Adding a GC base pair back in where the AT pair was deleted. Adding one base to the next codon and deleting one base from the one after that. Deleting a base pair from each of the next two codons.   A. 1,2 and 3 B. 1 and 3 C. 2 and 4 D. 4 only E. All of 1,2,3 and 4 are correct