1. You take a sample with a concentration of 1,000,000 pfu/mL of bacteriophage andmake a dilution by taking 1mL from the tube and add it to 9mL of fresh broth andmix. You and repeat this 4 more times, Then you plate 1mL of the sample with a fewdrops of E.coli on a TSA plate. How many plaques do you expect to see when youexamine the plate the next day?m^^^?Tube Aa) 1,000,000 plaquesb) 1000 plaquesc) 10 plaquesd) 1 plaquee) No plaquesE. coliTSA

Here, we are doing a dilution series to estimate bacteriophage concentration. One such technique is…

Answered: 1. You take a sample with a…

Microbiology for Surgical Technologists (MindTap Course List)

2nd Edition

ISBN:9781111306663

Author:Margaret Rodriguez, Paul Price

Publisher:Margaret Rodriguez, Paul Price

Chapter6: Microbial Viability And Growth

Section: Chapter Questions

Problem 4UTM

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Average plaques for bacteriophage A，B，C are 137，36，25. PFU/ml is average plaques multiply by the volume dilution multiply by the dilution factor. Show your working for each bacteriophage.
1. The colonies on a negative MSA plate would appear _____________. 2. "E. Coli and S. epidermidis were chosen to represent Gram-negative and Gram-positive bacteria, respectively. For a given antibiotic, is there a difference in susceptibility between the Gram-positive and Gram-negative bacteria?" "No, they are usually similar" "yes, drugs that target the cell membrane are more effective on gram negatives" "yes, drugs that target the cell wall are more effective on gram negatives" "yes, drugs that target the cell wall are more effective on gram positives" how can I tell the difference
29. Correctly label each image below with the ELISA method it is describing (3pts) Sand Ag Substrate ghta feel 30SICA S Primary Antibody Conjugate Substrate Secondary Antibody Conjugate You Substrate Capture Antibody Seeslo aidi onirif gnil 30. Andrew is a doctor in a hospital. He is treating a patient with a bacterial respiratory infection. He wants to know what the best antibiotic to prescribe for this infection is, so isolates the microorganism and does a Kirby-Bauer test. His results are pictured below. (3pts)
Your lazy labmate needs your help! They set up a 10-ml culture of the slow-growing Myxococcus xanthus yesterday, but then they decided to stay up late playing videogames and won't be in until next Monday to recover. Whoops! They call you in lab that Friday and ask you to take an OD reading for them. You do this favor for them, and helpfully do the conversion into cells/mL, finding that their culture is currently sitting at about 3.0 x108 cells per mL. Your labmate is horrified: they won't be back in time before the cells hit stationary phase, since M. xanthus doubles every 5 hours. They beg you to make a new culture using these cells and set it up to reach the same cell density Monday morning. That's 59 hours away. If the cells usually lag for three hours upon dilution into fresh medium, what cell density should you dilute the culture to? About 1.3 x 107 cells/ml About 1.3 x 105 cells/ml About 1.3 x 106 cells/ml About 1.3 x 104 cells/ml
Bacteriophage A average = 136 plaques Bacteriophage B average = 38 plaques Bacteriophage C average = 27 plaques Please help find the PFU/ml and answer the question on the best bacteriophage. Thanks
In lab we learned a technique that helped us to visulize individual colonies of bacter 1. Describe this technique. 2. What do you expect the resutls to look like? Be specific. 3. How can this technique help you to determine if your culture is contaminated? For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac). BIUS Paragraph I +] F H Ix X ABC † ( O K₂ KN Q V Arial sè "Ω Θ A 4 10pt EE 88 A Click Save and Submit to save and submit. Click Save All Answers to save all answers. 描く前 X² X₂ 3 由用目
6. Answer the following questions about colony forming units. a. b. You take a culture of bacteria and dilute it 5 times. Each dilution uses 1 ml of culture and 9 ml of saline solution. You take .1ml from the most dilute tube and plate it on a TSA plate. The next day you count 61 colonies. What was the original concentration a) 6.1 X 105 CFU/ml b) 6.1 X 105 CFU/ml c) 6.1 X 108 CFU/ml d) 6.1 X 107 CFU/ml You are doing a series of serial dilutions to find the concentration of bacteria in CFU/ml in a culture. You plate several of the dilutions tubes and count the colonies the next day. On plate A you find over 1000 colonies, plate B you find 157 colonies and plate C you find 7 colonies. Which plate(s) can be used to determine the concentration in the original tube? a) Plate A Ob) None of the plates can be used c) Plate C d) Plate B e) Plates A, B and C
1. You have a culture of yeast that is at a density of 8x107 cells/mL 2. A lab technician took a fecal sample and carried out the following dilutions: 1. Transferred 0.1 mL from the fecal sample 0.1 ml I ml 0.01 ml to 9.9 mL saline in tube A 2. Transferred 1 mL from A to 9 mL saline in tube B 3. Transferred 0.01 mL from B to 9.99 mL 0.1 ml A C saline in tube C 4. Transferred 0.1 mL from tube C to an agar plate 5. Counted 65 colonies on plate D 9.9 ml 9 ml 9.99 ml saline saline saline D 5. What is the concentration of cells in tube C? 6. What is the concentration of cells in the fecal sample? 1
What happens if you dont' add maltose in the molten top agar when performing an plaque assay? What would be the expected results?
1. You have a culture of yeast that is at a density of 8x107 cells/mL 2. A lab technician took a fecal sample and carried out the following dilutions: 1. Transferred0.1 mL from the fecal sample 0.1 ml 1 ml 0.01 ml to 9.9 mL saline in tube A 2. Transferred 1 mL from A to 9 mL saline in tube B 3. Transferred 0.01 mL from B to 9.99 mL saline in tube C 4. Transferred 0.1 mL from tube C to an agar plate 5. Counted 65 colonies on plate D 0.1 ml B 9 ml saline 9.9 ml 9.99 ml D saline saline Questions: 1. What is the serial dilution in A? 2. What is the serial dilution in B? 3. What is the serial dilution in C? 4. What is the total dilution in C? 5. What is the concentration of cells in tube C? 6. What is the concentration of cells in the fecal sample? 1
1. You wish to determine viable counts on a culture of Bacillus subtilis. You begin by pipetting 1 ml of culture into 99 ml of sterile water. After mixing the dilution well you make a series of 4 further dilutions of 10-1 each. From the three most dilute samples, you prepare three pour plates using 1 ml in each. After incubation you find the plate counts of the plates are 16, 245 and 890 respectively. a) Show the dilution scheme. b) What is the estimated viable count (cells/ml) in the original culture? 2. Scientific Notation. Fill in the missing information. a) 4.5 x 109 = _______ b) 50 x 107 = _______ c) 2300 x 1010 = _______ d) 0.54 x 108 = _______
Write the letter of your choice at the start of the sentence then support it with a short explanation. Plaque assay and Kirby Bauer assay are similar in terms of A. they both use a bacterial lawn B. zone of inhibition is measured C. antibiotics are used D. agar overlay is added on solidified agar

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