1. List 4 methods general methods for experimentally inhibiting the action of specific genes.

Q: What is the default state of the lac operon? a. LacI repressor is sitting on the operator sequence…

A: Lacl repressor is sitting on a operator sequence and blocks RNAP from transcribing lacZYA

Q: Name and describe two types of constitutive mutants of the lactose operon.

A: Operon can be defined as an operating unit, which is nothing but gene clusters that are located…

Q: 1.  List four general methods for experimentally inhibiting the activity of a specific gene with…

A: Gene inhibition is the regulation of gene expression in a cell that deactivates or silences or…

Q: Pick six “partial diploid” lac operon problems from the lac operon practice sheet.  (Those are the…

A: i) The repressor (l-d) is defective and can't binds with the operator. So, the system remains always…

Q: Think about the structure and transmission of F factors and discusshow you think F factors may have…

A: F factors are the F plasmid which are found in gram-negative bacteria and accounts for fertility.…

Q: Describe the main features of repressible operons.

A: An operon is defined as a particular set of genes present on a DNA that operates under same…

Q: Transposase protein cana. bind to DNA.b. catalyze the excision of a transposable element froma donor…

A: Introduction: Transposons are a region or DNA segment that possess the ability to replicate on its…

Q: Describe the terms: Transformation Operon Recombination

A: Hello you have asked question with multiple subparts. We will be able to answer only first three.…

Q: 3. Briefly explain how the Sanger technique for sequencing works.

A: Sanger sequencing, also known as the “chain termination method,” was developed by the English…

Q: Which of the following statements correctly identifies whether the indicated mutation is a…

A: Mutations are the sudden heritable changes that can occur in an organism or gene pool. Often…

Q: How does induction control enzyme synthesis using lactose operon?  2. Describe how level of a key…

A: By controlling the transcription of the mRNA which is needed for the enzyme synthesis, the genetic…

Q: 1. Explain why catabolite repression is used in regulating the lac operon and describe how it…

A: Repression by glucose of beta-galactosidase synthesis is spontaneously reversible in all strains of…

Q: You have discovered a new bHLH gene and would like to use ChIP-Seq to learn more about it's role in…

A: A basic Helix-Loop-Helix Domain (bHLH) is a very common domain found in proteins that act as…

Q: Mutations that confer no apparent selective advantage or disadvantage in a particular environment…

A: Introduction:- Mutations are defined as the changes in the DNA sequence or number that leads to…

Q: The effects of transposons can includeA. disruption of gene expression.B. prevention of gene…

A: Transposons are the genetic elements that have the ability to change their location within the…

Q: Describe how Ames Test works to identify carcinogenic using back mutations (reversible)

A: The Ames test is a widely employed method that uses bacteria to test whether a given chemical can…

Q: How can Cas9 technology be used in gene therapy. A. Base editing can be used to repair DNA error…

A:

Q: 1. Lac and Trp Operon are two different processes of sugar and amino acid. Describe the differences…

A: Lac and Trp operon are found in E. coli and other bacteria.

Q: 1. What is mutation? Explain the random and site-directed mutagenesis methods .Which one is…

A: Q1 a mutation is change in the sequence of the DNA or something which alters the genetic sequence of…

Q: What are some advantages and disadvantages of using mathematical models to study biological…

A: An operon is defined as a group of genes that has a common promoter and regulator and also…

Q: A mutation in one gene that reverses the phenotypic effects of amutation in a different gene isa. an…

A: A mutation is a change that occurs in deoxyribonucleic acid (DNA) sequence during the process of…

Q: Explain the The Operon Model: Negative Control ?

A: The lac operon of E. coli contains genes involved in lactose metabolism. It is expressed only when…

Q: 1) The UR operon which is composed of sequences A, B, C, and D (which may or may not be genes of…

A: An operon may be considered as a group of certain genes which function together so as to regulate…

Q: 5a. The table below summarizes expression of B-galactosidase (lacZ) and permease (lacY) from the lac…

A: 5a) Lac operon is an inducible operon that is expressed in the presence of a substance termed…

Q: The first person to receive gene therapy was a young girl with adenosine desminase(ADA) deficiency.…

A: Gene treatment endeavors to treat cancer or boost the body's capacity to combat illness by…

Q: A)Explain the mechanism by which the lac operon is sensitive to glucose levels (presence and absence…

A: “Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: 5f. Complete the diagrams below to reflect the proteins and any relevant cofactors (e.g. -…

A:

Q: 4. A mutant strain of E. coli produces B-galactosidase in the presence and the absence of lactose.…

A: An operon is defined as a set of structural genes regulated by a common promoter in bacteria.

Q: 1. Compare and contrast gene regulation of a Lac operon when there is little glucose in the…

A: The cellular environment has an impact on cellular action which is achieved by controlling the…

Q: Explain in detail the AMES test which is used to test for the mutagenic potential of different…

A: "Microbiology" is the study of microorganisms and associated topics. Microbiology has gone a long…

Q: Briefly explain the principle behind the following techniques: (a) ion-exchange chromatography (b)…

A: Since you have posted a question with multiple sub-parts, we will solve the first three subparts for…

Q: Explain how site directed mutagenesis can be accomplished using M13 bacteriophage. Using this method…

A: Deoxyribonucleic acid (DNA) is a hereditary molecule that passes genetic information from one…

Q: Outline the operon theory using the lac operon as anexample

A: Operon consists of genes placed in sequential order. Lac operon has following genes- Regulator gene…

Q: The ab initio approach finds genes by looking for a. common sequences found in most genes. b.…

A: Gene prediction is a computational biology process in which the encoding regions of a gene are…

Q: 23 Environmental factors typically activate genes in a cell by causing the cell to

A: The gene expression of a cell is controlled by transcription and translation which helps in the…

Q: The purpose of a ChIP-chip assay is to determinea. the expression levels of particular genes in the…

A: Introduction Genes are the particular sequence of nucleotides which gets expressed and give rise to…

Q: You are studying celiacs disease and have identified a potential gene for the disease using linkage…

A: Genetics is the study of the heredity, expression of genes and control of phenotypes. There are…

Q: Describe the advantages and disadvantages for organisms that arrange genes in operons.

A: Operons are regions of DNA that contain clusters of related genes and are made up of promoter…

Q: Unusually high levels of Mdr1 provide stem cells with a. Nutrients needed b. Protection of their…

A: Stem cells are cells that has the ability to differentiate into different cells found in the body.

Q: the application of publishing the epigenome roadmap results? 2. List global maps established by…

A:

Q: 6 of 16 Which gene would most likely be under inducible expression control?

A: There are two types of expression systems of genes they are constitutive expression system and…

Q: Describe a biotechnology technique(s) that you could use to study how active (how much transcription…

A: Introduction: Transcription is a process in which DNA is transcribed into mRNA. It is a four-step…

Q: Why would a scientist wish to induce mutations?   a. to increase the number of mutated phenotypes…

A: Induced mutations are alterations in the gene after it has come in contact with mutagens and…

Q: How can the construct with Cas9 and gRNA sequence be removed from the plant in case it was inserted…

A: Introduction CRISPR-Cas9 is a genome editing tool that's creating a buzz in the science world. It's…

Q: 3 5. Suppose you perfe would you expect 6. If you did not get the operon was c

A: B -Galactosidase -- B - galactosidase belongs to class 3 ,hydrolases .B gal belongs to subclass of…

Q: Highlight the main points of lac operon operation.

A: In prokaryotes, gene regulation is most prominent during the start of transcription. As a result,…

Q: Compare and contrast repressible and inducible operons. 2. Are genes for enzymes involved in…

A: An operon is a group of genes coding for proteins that perform related functions, that are…

Q: b) Organic matters may interfere with heat treatment of bacterial growth control. ( c)…

A: Please follow step 2 for detailed explanation.

Q: Which of the following methods is used to detect a specific RNAwithin a mixture of many different…

A: Blotting is a hybridization technique, which helps in identifying different biomolecules such as…

Q: What are the effects of the following conditions on Lac operon of bacteria

A: Lac Operon is a set of three structural genes regulated by a common promoter and maintain lactose…